Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,375,828	IS186 (+) +4 bp	coding (132‑135/963 nt)	menC ←	o‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2375828 =	1 (0.040)	23 (0.840)	23/480	0.3	95.8%	coding (135/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	= 2515617	NA (NA)					noncoding (1345/1345 nt)	IS186	repeat region
*	?	NC_000913	= 2375831	0 (0.000)	24 (0.880)	24/480	0.3	100%	coding (132/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region

CCACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2376062‑2375828 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGG  <  NC_000913/2515617‑2515389                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    CCACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAAACCGCGAC‑GGGGTGTATGTTTGCCTGCGTGAAGGCGAGCGCGGAGGGGGGGGGGAGATCTCCCATAAGCGCTAAC                                                                                                                                                                                                                        >  1:274953/1‑251      ATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAG                                                                                                                                                                                                                   <  2:157002/251‑1       TGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAACCGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGGTAAAAACCCGCGGC‑GGGGTGTTTGTTTGCCTGCGGGGAGGCGGGCGCGACGGGGGGGGGGGGATCTCCCATAAGGGCTAACTTCAGG                                                                                                                                                                                                                  >  2:164581/1‑251                  agGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTTTATGTTTGCCTTCGTGAAGGGGGGCGCTAATGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATC                                                                                                                                                                                                       <  1:43344/249‑1                                              ACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAAACCCCGAC‑GGGGTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGGGGGGGGAGATCTTCCCTAAACGGTAACTTAAGGGGTGAAACATCTGGAGGATGCGACGCCTCGGGTCCTCGT                                                                                                                                                                           >  1:40944/1‑251                                               CGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTT                                                                                                                                                                          <  1:56099/251‑1                                                     CAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAAGGCGTGGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACG                                                                                                                                                                    <  2:128594/251‑1                                                               CGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAAACCGCGACCGGGC‑GTATGTTTGCCCGCGTGCAGGCGAGCGCGAAGGGTGGGGGGGGATCTCCCATCAGCGCTAAATTAAGGGGTGAACCATCTGAAGAATGCGACGCCTCCGTGCCTCGGTAAGACGATGCCTCccg                                                                                                                                                          >  2:197926/1‑248                                                                    GCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTT                                                                                                                                                     <  2:123873/251‑1                                                                                  AATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGT                                                                                                                                      <  1:249677/251‑1                                                                                           GGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATGGC‑GTTTTGTAGGCTGTCA                                                                                                                             <  2:57663/251‑1                                                                                                    AATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGGTGTATGTTTGGCTGCGTGGAGGCGAGCGCGGAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGAC                                                                                                                                                                                          >  2:27181/1‑182                                                                                                    AATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTG                                                                                                                    <  1:23183/251‑1                                                                                                    AATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGAC                                                                                                                                                                                          <  1:27181/182‑1                                                                                                                                CAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGGGAAGGCGAGCGCGAAGGGGGGGGGGGGATCTCCCATAAGCGCTAACTTAAGGGGTGAACCCTCTGAAGAATGCGACGCCTCCGTGCCTCGTTAAGACGATGCCTCGCGGTTTTCAATTGC‑GTTTTTTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCc                                                                                        >  1:194039/1‑250                                                                                                                                         ATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGGGGTCCCCCATAAGCGCTAACTTAAGGGGTGAACCATCTGGAGAATGCGACGCCCCCGTGCCTCGTTAAGACGATGCCCCGCGGTCTTCCATTGCCGTTTT‑TAGGGTGTCCGGGATACTGTCCCACGAAAGGCCACCCTTAAGCTCCCGATGACCAT                                                                               >  2:83900/1‑251                                                                                                                                                        GTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCA                                                                <  2:27585/251‑1                                                                                                                                                             GCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACA                                                              <  1:237310/248‑1                                                                                                                                                                                  CCGCGAC‑GGGCTGTATGGTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGAT                                      <  2:254123/251‑1                                                                                                                                                                                          GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGTTGGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAATACGATGGCTCGCGTTCTGCAATTGC‑GTGTTGTAGGCTGGCAGGGATGCTGTCCCGCGAAGGGCCACCTGTAAGCTCCAGATGACCGTTTTTGTTAGTCTCCACAACGAGTTAGTTCTGCTTTTCGGATCCGGCAC                               <  1:14162/251‑1                                                                                                                                                                                               GTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTGTCGGATCCGGCACTTCT                           <  1:204007/250‑1                                                                                                                                                                                                        CCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAA                  >  1:6994/1‑250                                                                                                                                                                                                                       GCGCGAAGGGTGGGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGG  <  1:104062/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    CCACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGAC‑GGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCtccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2376062‑2375828 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCCATAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGC‑GTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTTTTGTTATTCTCCACAACGAGTTAGTTCTTCTTTTCGGATCCGGCACTTCTGGGGGGGAAATCCAGCGATGGCTGG  <  NC_000913/2515617‑2515389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.