Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,631,612	IS5 (+) +4 bp	coding (976‑979/1623 nt)	yhiJ ←	DUF4049 domain‑containing protein YhiJ

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	27 (0.970)	26/488	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3631612 =	0 (0.000)					coding (979/1623 nt)	yhiJ	DUF4049 domain‑containing protein YhiJ
*	?	NC_000913	= 2290113	NA (NA)	33 (1.190)	33/488	0.1	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3631615	0 (0.000)					coding (976/1623 nt)	yhiJ	DUF4049 domain‑containing protein YhiJ

TGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289156‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGC  >  NC_000913/3631612‑3631856                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     TGAAAAAAGATAAATAACTGGAGCTGTTATTAACGGTAGCCCACCTGTTTCGGACGGACAAACTGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGCTTCAAGGCATTTACGGGAGAAAAAATCGGCTCCAACATGAAGAAATGACATGCCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAATACATGAAC                                                                                                                                                                                                                                          >  2:119293/1‑251   AAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGTGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACAT                                                                                                                                                                                                                                        >  2:204119/1‑251              CCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATA                                                                                                                                                                                                                             <  2:114141/251‑1                    GGCGATGTTATTCAATCTGACTAACCTGTTTCGGGCGGACAAAATGATACGTCAGTGGGCGAGATCTCACTAAAAACTGGGGCTAACGCCTTAAAAGGCTAAGAAACGGTCTAAATAGGCTGATACAAGGCATTTACGGGAGAAAAAATCGGCTCAAACCTGAATAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACATTCCCTAATACATGAACATTTTCATTGATACTCATT                                                                                                                                                                                                                       >  2:253563/1‑251                         TGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCG                                                                                                                                                                                                                  >  1:247117/1‑251                                                  TCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCAGTATGCGTTTTAACAAATCGATGATAAATTG                                                                                                                                                                                          <  2:40964/250‑1                                                   CGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTGATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGAT                                                                                                                                                                                        <  2:145344/251‑1                                                                 GATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTG                                                                                                                                                                          <  2:94200/251‑1                                                                        CAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATC                                                                                                                                                                   <  2:29188/251‑1                                                                             GGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCG                                                                                                                                                              <  2:116/251‑1                                                                                       CACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGT                                                                                                                                                    >  1:278247/1‑251                                                                                                                                    AAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAA                                                                                                          <  2:203858/248‑1                                                                                                                                      ATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCGCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACAGGGCGTCACAGCGTCTTTATTAATAACTAAA                                                                                                     <  2:206811/251‑1                                                                                                                                         GGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGCGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGCTCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATA                                                                                                   >  2:246709/1‑250                                                                                                                                                                              CAAAAATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTC                                                              >  2:205316/1‑250                                                                                                                                                                                           ATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTATGTTCAGGATACTGATTAT                                                >  2:25547/1‑251                                                                                                                                                                                                ATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTAT                                           <  1:86971/251‑1                                                                                                                                                                                                  GACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATT                                          <  1:4990/250‑1                                                                                                                                                                                                              CGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTGCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTA                             <  2:275286/251‑1                                                                                                                                                                                                                   AGAATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACCTTTTCATCGATACTCATTATGCGTTTTAACAAATCGATGCTAAATTGATCACCACAGATCTTGATAAATCGATCGCCGGTTTGGTCGCCCAGAAAAGTACATGGCGTCACAGCGTCTTTATTCATAACTAACTAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGCTACTGATTATTGTATTAACATTAGTGTTAAGTTG                        >  1:142405/1‑251                                                                                                                                                                                                                     AATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCT                      >  1:95992/1‑251                                                                                                                                                                                                                      ATTTCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTC                     <  2:247117/251‑1                                                                                                                                                                                                                         TCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCAT                   <  1:204119/250‑1                                                                                                                                                                                                                         TCCCCGCTTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCAT                   <  1:116605/250‑1                                                                                                                                                                                                                                TTATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTC           >  1:151045/1‑251                                                                                                                                                                                                                                  ATTCGCACCTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCT          <  1:244414/250‑1                                                                                                                                                                                                                                          CTTCCCTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGC  >  1:224815/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     TGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289156‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATACATGAACATTTTCATTGATACTCATTATGCGTTTTAACAAATCGATGATAAATTGATCACCACAGATATTGCTAAATCGATCGCCGGTTTGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGC  >  NC_000913/3631612‑3631856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.