| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,302,204 | Δ1 bp | coding (1023/1632 nt) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,302,204 | 0 | T | . | 96.7% | 126.8 / ‑3.9 | 30 | coding (1023/1632 nt) | oppA | oligopeptide ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (16/13); minor base C (1/0); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGT‑TACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCA > NC_000913/1301969‑1302443 | gTAGTGGAGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCC‑TACCCTGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGTCTA‑GGTATACACTCCACCg < 2:31358/251‑1 (MQ=255) aCATGCCGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGACGTTCACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGgcgc > 2:167204/1‑250 (MQ=255) gacgtataaaaccATGCCGATCGCATTGTTCCAGAAGCTGCAAAAAGAGCTCCCGGACGAAGTTCACGTTGATCCATACC‑TGTTCACTTACTATTACGAACTTAACAACCAGAAACAGCCATTCAACGATGTGCGTGTGCGTACCGCACTGACACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAAt > 2:276467/13‑251 (MQ=255) gACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAAt > 1:102638/1‑251 (MQ=255) acaccactctgtaccgaacaaactGTTACAGAAGCCGAAAAAAAAAGTCCCGGCCGACGTTCACGTTAATCCATCCC‑TGTGATTTTGCTATTCCGAAATTACCAACAAGAAGCCGCCATTCAACGATGTGCGTGTGCGTACCGCATTGAAACTAGGTAGGGACGGCGATATCATTGTTAATAAAGTTAAAGCGCAGGGTAACATGCCCGCCCA‑GGTATTCACGCCACCGTATATTGATGGCGCAAAAtt < 2:255253/226‑1 (MQ=255) tCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGttt > 1:69752/1‑251 (MQ=255) aaTTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATACATCAC‑TGTGCACTTACTATTACGAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGAGCAGGGCAACATGCCCGCCT‑CGGTATACACTCCACCGTATACTGATGGCGCAAAAtt > 1:255253/1‑229 (MQ=255) ttCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTAACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTgg > 1:164551/1‑249 (MQ=255) ttCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTACCCGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGa < 2:165330/250‑1 (MQ=255) aTCCCGGACGAAGTTCACGTTGATCCATACC‑TGTGCACTTACTCTTACGACCTTAACAACCAGCAACCGCCATTCCACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATCTCATTGTTAATAAAGTGAAAGCGCAGGGCACCATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAACCACGTAACga > 1:119854/1‑248 (MQ=255) tCCCGGACGAACTGCGCCTTGATCAATACC‑TGTGCACTTCCTATTACGAAATTAACAACCAGAAACCGCCACTCACCGATGTGCGTGCGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGCCTCAGCCAGAATGGTTTGGCTGGCGCCAGGAAAAACGTAACgaaga > 2:20801/1‑250 (MQ=255) tgacccATACC‑GGGGCACGTACTATTACGAAATTAAAAACCAGAAGCCGCCATTCAACGATGGGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACTAAGAAGCGAAAAAGCTgctggct < 2:272549/246‑1 (MQ=255) gCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATAcc > 1:145320/1‑251 (MQ=255) tttcaatcaCGAAATGACCAACAAGAAACCGCCATTCAAGGATGTGCGTGTGCGTCCCGCACGGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAACCGCAAGGCAACATGCCCGCCTA‑GGTATCCACTCCACCGAATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCa < 2:217584/242‑1 (MQ=255) aaTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGAAACATGCCCGCCTA‑GGTATACACTCAACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAGCGAAGAATTGAAAAAACTTCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGa < 1:142645/251‑1 (MQ=255) ccAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGATGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAAcc > 1:134866/1‑251 (MQ=255) gCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGtat > 1:180530/1‑250 (MQ=255) aTTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAac > 1:255912/1‑250 (MQ=255) cgtgtgcgtACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCa < 1:217482/251‑1 (MQ=255) aCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCg > 2:147964/1‑250 (MQ=255) aaaCTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATtgc < 2:15702/251‑1 (MQ=255) tATGGACCGCGCTATAATTGTTACTAACGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCtt > 2:176259/1‑251 (MQ=255) cgcgATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATtgtg < 2:55842/251‑1 (MQ=255) cATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGTATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAAAACCTCCGATCTGCATAAAAAGCTGGCGATTTCTGCCTCTTCATTGTGGAAGaaa > 1:174563/1‑250 (MQ=255) cATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAATCGGGTTATACCGCAGACAAACCGTCGACCATCAACCTGTTGTATAACACCTCCGAACTGCATAAAAAGCTGGCGATGGCTGCCTCTTCATTGTGGAAGaaa < 2:137818/250‑1 (MQ=255) tGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAAc < 1:218036/250‑1 (MQ=255) ataaAGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGgt < 2:102638/251‑1 (MQ=255) aaaGTGAAAGCGCAGGGCAACATGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGgtgt > 1:78537/1‑251 (MQ=255) aGGGCAACATGCCCGCCTA‑GGTATACACTCCAACGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCCAACTg > 2:272981/1‑251 (MQ=255) aTGCCCGCCTA‑GGTATACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCa < 1:132659/251‑1 (MQ=255) | GTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACC‑TGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGT‑TACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCA > NC_000913/1301969‑1302443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |