Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,935	G→A	S467N (AGT→AAT)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,935	0	G	A	100.0%	315.0 / NA	91	S467N (AGT→AAT)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (58/33);  total (58/33)

ATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTG  >  NC_000913/487640‑488230                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        atctcgGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAAc                                                                                                                                                                                                                                                                                                     <  2:475153/296‑1 (MQ=255) aTCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAAc                                                                                                                                                                                                                                                                                                     <  1:70589/300‑1 (MQ=255) aTCGCGCATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATACGCGTCGCGAATTGCTGGATCAACTCANCAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCNGACTCAGCAAATCTTTTGGGTGAACAATAAc                                                                                                                                                                                                                                                                                                     <  2:594521/300‑1 (MQ=255)  tCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAAcc                                                                                                                                                                                                                                                                                                    <  1:6002/300‑1 (MQ=255)  tCGCGGATTTGCGTCTAGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAAACAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAAcc                                                                                                                                                                                                                                                                                                    >  2:463315/1‑300 (MQ=255)      ggATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCNAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTcc                                                                                                                                                                                                                                                                                                <  1:592242/300‑1 (MQ=255)           tGCGTCTCGAACAGTTTTAAGTTAACCAGCAGTGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACATCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATgg                                                                                                                                                                                                                                                                                           <  2:164785/300‑1 (MQ=255)           tGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCATAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATgg                                                                                                                                                                                                                                                                                           <  2:645469/300‑1 (MQ=255)              gTCTCGAACAGTTTGAAGTTACCCAGCAGCGTGATGCACTCTTACAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACAACGTCAACAGCGAAGGTCAAGATGCGTTATTGCAAGTGGTTGATATGCGTGGAGAATTGCTGGATTAACTAAACAACNAGTTGGGTAACCAGCTGATGATGGCAATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTAGAAAAACCTGAAGTCCATCCTGACTAAGCAAATCTTTTGAGTGAACAATAAACGtaaaagggaag                                                                                                                                                                                                                                                                                        >  2:206807/1‑290 (MQ=255)              gTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACTTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATgaact                                                                                                                                                                                                                                                                                        >  2:406578/1‑300 (MQ=255)              gTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATggact                                                                                                                                                                                                                                                                                        >  1:239151/1‑300 (MQ=255)                   gAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGGTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATggactgggac                                                                                                                                                                                                                                                                                   <  2:652880/300‑1 (MQ=255)                        ggttGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGAt                                                                                                                                                                                                                                                                              <  1:681901/298‑1 (MQ=255)                        gTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCCAACAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGAATGGGACTGGAt                                                                                                                                                                                                                                                                              >  2:148283/1‑300 (MQ=255)                        gTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGAt                                                                                                                                                                                                                                                                              <  1:639146/300‑1 (MQ=255)                        gTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCAAGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCTAATGGACTGGGACTGGAt                                                                                                                                                                                                                                                                              >  1:186392/1‑300 (MQ=255)                             aaGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAg                                                                                                                                                                                                                                                                         <  1:762133/300‑1 (MQ=255)                                            gTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGNTGAACAATAACCGTCCAATGGACTGGGACTGGATCAACGCGTTCCCGNAACGcc                                                                                                                                                                                                                                                          >  2:701146/1‑300 (MQ=255)                                            gTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGNTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGNAAAgaa                                                                                                                                                                                                                                                          >  2:701596/1‑298 (MQ=255)                                            gTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGcc                                                                                                                                                                                                                                                          >  1:480171/1‑300 (MQ=255)                                            gTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGANCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCNGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACGATAACCGTACAATGGAATGGGACTGGATCAAAGCGTTCCCGCAAAGca                                                                                                                                                                                                                                                          >  2:686245/1‑299 (MQ=255)                                            gTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGCTGCTATGCGTCGCGGATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAAATAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCACATCTCTTGGGTGGACAATAACTGTAAACTGACCTGGGACTGGATCAAAGCGtccacgcagaatc                                                                                                                                                                                                                                                          >  2:232982/1‑288 (MQ=255)                                            gTGATGCACTCTTACAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTNACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGCACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCACGCAAAGcc                                                                                                                                                                                                                                                          >  2:197577/1‑300 (MQ=255)                                                  cACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAg                                                                                                                                                                                                                                                    <  2:497374/300‑1 (MQ=255)                                                      ctTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCATGAAAGATGa                                                                                                                                                                                                                                                >  2:419187/1‑300 (MQ=255)                                                      ctTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGa                                                                                                                                                                                                                                                >  1:51541/1‑300 (MQ=255)                                                      ctTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCATGAAAGATGa                                                                                                                                                                                                                                                >  2:496425/1‑300 (MQ=255)                                                      ctTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTACAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCATGAAAGATGa                                                                                                                                                                                                                                                >  2:800330/1‑300 (MQ=255)                                                      ctTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCAAGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGCTCCCGCAAAGCCTGAAAGATGa                                                                                                                                                                                                                                                >  2:154085/1‑300 (MQ=255)                                                       ttCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGaa                                                                                                                                                                                                                                               >  1:468727/1‑300 (MQ=255)                                                       ttCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGGTATTGCAAGTGGTTGATATGTGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTACAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGAtaga                                                                                                                                                                                                                                               >  2:453278/1‑297 (MQ=255)                                                                      ttCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCCAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAACGCCTGAAAGGTGAATTTAAGTCAATGaaa                                                                                                                                                                                                                                >  1:302737/1‑300 (MQ=255)                                                                        cGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAAt                                                                                                                                                                                                                              >  1:71989/1‑300 (MQ=255)                                                                                  cTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAAc                                                                                                                                                                                                                    >  1:844547/1‑300 (MQ=255)                                                                                       agaagGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCa                                                                                                                                                                                                               <  1:536297/300‑1 (MQ=255)                                                                                          agGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCNGCAAAGCATGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTNGCaaaa                                                                                                                                                                                                            >  2:691388/1‑300 (MQ=255)                                                                                             tCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGc                                                                                                                                                                                                         <  1:839218/300‑1 (MQ=255)                                                                                                cacCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTg                                                                                                                                                                                                      >  1:499286/1‑300 (MQ=255)                                                                                                          gTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCACGCCGtt                                                                                                                                                                                            >  1:723643/1‑300 (MQ=255)                                                                                                              aCAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGNATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAANTTAAGTCGATGAAAATCACGGTGCACTGGCAAAAAGCCTGGCCCGCCGTTTTTa                                                                                                                                                                                        >  2:776051/1‑300 (MQ=255)                                                                                                              aCAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTa                                                                                                                                                                                        >  1:249111/1‑300 (MQ=255)                                                                                                                      gTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAAACTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCATGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTCTATCGCTTTc                                                                                                                                                                                >  2:46306/1‑300 (MQ=255)                                                                                                                           cGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGc                                                                                                                                                                           >  1:386906/1‑300 (MQ=255)                                                                                                                               gCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCATGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCtagt                                                                                                                                                                       >  2:136290/1‑297 (MQ=255)                                                                                                                                cGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGtt                                                                                                                                                                      >  2:109933/1‑300 (MQ=255)                                                                                                                                 gTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCAAGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGttt                                                                                                                                                                     >  2:66005/1‑300 (MQ=255)                                                                                                                                             ggTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCAAGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGtt                                                                                                                                                         >  2:757628/1‑300 (MQ=255)                                                                                                                                              gTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCATGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCACGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTg                                                                                                                                                        >  2:399916/1‑300 (MQ=255)                                                                                                                                              gTTGATATGCGTCGCGAATTGCTAGATCAACTCAACAAACAGTTAGGTAACCAGCTGATGATGGCCATTCACCTGCAAATCAACCCGCAGCAGTTAATGAGTGTGTCGCAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTACGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTg                                                                                                                                                        >  1:564787/1‑300 (MQ=255)                                                                                                                                                tGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCGGTTGAt                                                                                                                                                      <  1:271661/300‑1 (MQ=255)                                                                                                                                                   tatGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGc                                                                                                                                                   <  2:186930/300‑1 (MQ=255)                                                                                                                                                             gAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATc                                                                                                                                         <  2:311827/300‑1 (MQ=255)                                                                                                                                                              aaTTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATCAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATcc                                                                                                                                        <  2:725517/300‑1 (MQ=255)                                                                                                                                                                ttGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCAc                                                                                                                                      >  1:227208/1‑300 (MQ=255)                                                                                                                                                                              caacaaACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGggctg                                                                                                                        >  1:105644/1‑300 (MQ=255)                                                                                                                                                                              caacaaACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCAAGGTGAACTGGCAAAAAACATGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGggctg                                                                                                                        >  2:441858/1‑300 (MQ=255)                                                                                                                                                                                     caGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGaaa                                                                                                                 >  1:402878/1‑300 (MQ=255)                                                                                                                                                                                      aGTTGGGTAACCAGCTGATGATTGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTTGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGGAAg                                                                                                                <  1:806933/300‑1 (MQ=255)                                                                                                                                                                                        ttGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCg                                                                                                              <  1:693119/300‑1 (MQ=255)                                                                                                                                                                                          gggTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTa                                                                                                            >  1:526571/1‑300 (MQ=255)                                                                                                                                                                                            gTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATc                                                                                                          <  2:16361/300‑1 (MQ=255)                                                                                                                                                                                                 cAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCANAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaacaa                                                                                                     >  2:745908/1‑300 (MQ=255)                                                                                                                                                                                                   gCTGATGATGGCCATTAACCTGCAACTCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCACGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTATGGGCTGGCTGAAAGCGTATCAaaaaaa                                                                                                   >  2:42396/1‑300 (MQ=255)                                                                                                                                                                                                   gCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTCTCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACaaaa                                                                                                   >  1:241588/1‑300 (MQ=255)                                                                                                                                                                                                   gCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACNGGATCAAAGCGTTCCCGCAAAGCCTGNAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATcaaaacaa                                                                                                   >  2:574541/1‑295 (MQ=255)                                                                                                                                                                                                      gatgatGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGNCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACt                                                                                                >  2:730684/1‑300 (MQ=255)                                                                                                                                                                                                            ggCCATCAACCTGCAAATCAACCAGCAGCAGTTCATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCATGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTc                                                                                          >  1:397142/1‑300 (MQ=255)                                                                                                                                                                                                                 ttAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCtg                                                                                     <  1:1411/300‑1 (MQ=255)                                                                                                                                                                                                                          aaaTCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTccc                                                                            >  1:826892/1‑300 (MQ=255)                                                                                                                                                                                                                          aaaTCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTccc                                                                            <  2:728429/300‑1 (MQ=255)                                                                                                                                                                                                                                        cagTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagc                                                              >  1:181230/1‑300 (MQ=255)                                                                                                                                                                                                                                          gTTAATGAGTGTGTCGAAAAACCTGAAATCCNTCCTGACTCAGCAAATCTTTTGAGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTNAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGATTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagcca                                                            >  2:613855/1‑300 (MQ=255)                                                                                                                                                                                                                                          gTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGGTGATCCCCTGGCGTCTGGGCTGGCTGANAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagcca                                                            >  2:624986/1‑300 (MQ=255)                                                                                                                                                                                                                                          gTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTCTCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagcca                                                            >  1:716155/1‑300 (MQ=255)                                                                                                                                                                                                                                          gTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAcagcca                                                            >  1:128468/1‑300 (MQ=255)                                                                                                                                                                                                                                            tAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGAaagccagc                                                          >  2:166460/1‑300 (MQ=255)                                                                                                                                                                                                                                                   tgtgtCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAacac                                                   <  1:673591/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gtCGCAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACAcc                                                >  1:147371/1‑300 (MQ=255)                                                                                                                                                                                                                                                           aaaaCCTGAAATCCATCCTTACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAg                                           <  2:161601/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATcc                                     <  1:441522/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    aaTCCATCCTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTa                                  >  1:18420/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          tccTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACNGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGAcc                            <  1:581509/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tccTGACTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGAcc                            <  1:49474/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCTCAGTAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATc                       <  2:24783/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCTCAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATc                       <  1:244601/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cAGCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGt                    >  2:501938/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCAAATCTTTTGGGTGAACAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTgc                  <  2:243392/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tGGGTGAACAATAACCGTCCAATGGACTTGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTCTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACCGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAGCGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGgtg        <  2:241173/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tGGGTAAACAATAACCGTCCAATGGACTGAGACTTGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGACCCACCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGgtg        <  2:222525/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    aaCAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTg  >  1:749881/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    aaCAATAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGAACTGATCCGTGCGCTGCCGGTGTGCCTg  >  1:494286/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        ATCGCGGATTTGCGTCTCGAACAGTTTGAAGTTAACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTG  >  NC_000913/487640‑488230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑