Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,176,012	T→C	H23H (CAT→CAC)	tufB →	translation elongation factor Tu 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,176,012	0	T	C	100.0%	231.0 / NA	66	H23H (CAT→CAC)	tufB	translation elongation factor Tu 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/34);  total (32/34)

CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCACCGGTG  >  NC_000913/4175721‑4176229                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                          cGGGTTCGAGTCCCGCTGCCCGCTCCAGGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGTTAAGGTTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGTGCATGTTGCCTGGTTGATGTGGTGATATCACCAATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaac                                                                                                                                                                                                                   <  2:221887/300‑1 (MQ=39)      tCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGNACCCTTGGTAAGGTTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTANAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaactactc                                                                                                                                                                                                              <  2:785513/300‑6 (MQ=255)      tCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaactactc                                                                                                                                                                                                              <  1:29388/300‑6 (MQ=255)      tCGATTCCCACTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaactactc                                                                                                                                                                                                              <  2:503829/300‑6 (MQ=255)             ccGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGc                                                                                                                                                                                                       <  1:28827/300‑1 (MQ=255)                cTGCCCGCTCTAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGTTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGc                                                                                                                                                                                                    <  2:375800/300‑1 (MQ=255)                cTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGc                                                                                                                                                                                                    <  1:123527/300‑1 (MQ=255)                       cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTAAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTACATGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCATGTTAACGTTGGTACTATCGGCCACGTTGACCACGATAAAACTACTCTGAACGCTGCACTCAcc                                                                                                                                                                                             >  2:221904/1‑299 (MQ=38)                         ccAAGATGTGCTGAGATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGGTTATGGCATTCAACAAGTCGGGCATGTTGCCTGGTTGAGTTGGTGATATCACCGATTTATCCGTGTCTTATAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCAccac                                                                                                                                                                                           <  1:404348/300‑4 (MQ=37)                              aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTAc                                                                                                                                                                                      <  1:213219/300‑1 (MQ=255)                                   gagacagaGCTCAGTTGTTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCt                                                                                                                                                                                 <  2:124390/293‑1 (MQ=37)                                   cTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCt                                                                                                                                                                                 <  1:478551/300‑1 (MQ=255)                                     gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGAACGCTGCAATCACCACCGTACTGGCTaa                                                                                                                                                                               >  1:131648/1‑300 (MQ=255)                                      atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCTGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTaaa                                                                                                                                                                              >  1:340204/1‑300 (MQ=255)                                        ataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAc                                                                                                                                                                            <  2:61379/300‑1 (MQ=255)                                        ataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAc                                                                                                                                                                            <  1:378938/300‑1 (MQ=255)                                          aGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTACTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCtctgtctc                                                                                                                                                                          >  1:124853/1‑293 (MQ=37)                                              cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGCAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTAcgg                                                                                                                                                                      >  2:95308/1‑300 (MQ=255)                                                    gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGtgc                                                                                                                                                                <  1:443238/300‑1 (MQ=255)                                                         gcgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACNTCTTTTCTCCTCCCTTTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAANTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTc                                                                                                                                                           <  2:786939/300‑1 (MQ=37)                                                              cccTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCa                                                                                                                                                      >  2:177185/1‑300 (MQ=255)                                                              cccTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCa                                                                                                                                                      >  1:345784/1‑300 (MQ=255)                                                                cTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCTGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCAAGTTGACCACGGTAAAACTACTCCGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGTGGTGCTGCCcgcgtagt                                                                                                                                                    >  2:300569/1‑296 (MQ=37)                                                                  tGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACACAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCg                                                                                                                                                  >  1:345335/1‑300 (MQ=255)                                                                  tGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCg                                                                                                                                                  >  1:675205/1‑300 (MQ=255)                                                                    gTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGGTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAc                                                                                                                                                <  1:777061/300‑1 (MQ=255)                                                                        gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGa                                                                                                                                            >  2:411127/1‑300 (MQ=255)                                                                        gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGa                                                                                                                                            >  1:739206/1‑300 (MQ=255)                                                                        gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGa                                                                                                                                            >  1:137902/1‑300 (MQ=255)                                                                        gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCAACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGa                                                                                                                                            >  1:217996/1‑300 (MQ=255)                                                                         ggTGAGTTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTNTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGGTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCNATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGAt                                                                                                                                           <  2:793482/300‑1 (MQ=25)                                                                         ggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGAt                                                                                                                                           >  1:306816/1‑300 (MQ=255)                                                                               gTCGGCAGTTCGAATCTGCCTATTAGCACCACTTCTTTTCTCCTTCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGTACATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAATAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATaa                                                                                                                                     <  1:182608/300‑1 (MQ=25)                                                                                                gCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACCATCGATGTCTAAAGAAAAGTTTGAACGCACAAAACCGCACGTTAACGTTGGTCCTATCGGCCACTTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAACTACGGCGGTGCTGCTTGCGCATTCGACCAGATCGATAACGCGCCGGAAAAAAAAg                                                                                                                    >  2:283929/1‑300 (MQ=21)                                                                                                   tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCGATCACCACCGTACTGGCTAAAACCTACNGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTc                                                                                                                 >  2:625227/1‑300 (MQ=255)                                                                                                   tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTc                                                                                                                 >  1:776518/1‑300 (MQ=255)                                                                                                              cttcttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCAcc                                                                                                      >  1:709527/1‑300 (MQ=255)                                                                                                               ttcttTTCTCCTCCCTTTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACANTCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCa                                                                                                     <  1:612762/300‑1 (MQ=255)                                                                                                               ttcttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCANGTTAACGTTGGTACTATCGGCCACGTNGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCa                                                                                                     <  2:559068/300‑1 (MQ=255)                                                                                                                 cttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCAAGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGNGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATc                                                                                                   >  2:637622/1‑300 (MQ=255)                                                                                                                      ctcctcCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAacac                                                                                              <  2:539129/300‑1 (MQ=255)                                                                                                                                         tCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGac                                                                           <  1:415832/300‑1 (MQ=255)                                                                                                                                            gTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCCCCATCAACACTTATCACGTTGAATACGACAcc                                                                        >  1:673598/1‑300 (MQ=255)                                                                                                                                            gTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACAcc                                                                        >  1:801360/1‑300 (MQ=255)                                                                                                                                            gTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGAACGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCAACATCAACACTTCTCACGTTGAATACGACAcc                                                                        >  1:60917/1‑300 (MQ=255)                                                                                                                                              ttATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTAACGTTGAATACGACAcccc                                                                      >  1:319188/1‑300 (MQ=255)                                                                                                                                                             aGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGc                                                       <  1:710741/300‑1 (MQ=255)                                                                                                                                                                       ggtGCCTGGTTGATGTGTTGATATCACCGATCTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAGAACCGCACGTTAACGTTGGTACTATCGGCCAAGTTGACCACTGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTACTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  2:130305/298‑1 (MQ=14)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACTGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  1:92893/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  1:121200/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  2:758390/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGNCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTNCGGCGGTGCTGCTCGCGTATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  2:668874/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  1:121333/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  2:43683/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTGAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCAGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  1:167703/300‑1 (MQ=255)                                                                                                                                                                       gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTGAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTANCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                             <  1:629253/300‑1 (MQ=255)                                                                                                                                                                         tGCCTTGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTg                                           <  1:69809/300‑1 (MQ=255)                                                                                                                                                                             tGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCg                                       >  1:828455/1‑300 (MQ=255)                                                                                                                                                                               gTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTAAGGCGGTGCTGCTCGCGTATTCGACCAGATCGATAACGCGCCGGGAGAAAAAGCTCGTGGTATCAACATCAACACCTNCCACGTCGAATACGACACCAAGACCCGTCAATACGCACATGTAGACtnacgggg                                     >  2:701411/1‑293 (MQ=14)                                                                                                                                                                                 ggATGTGTTGATATCACCGATTTATCCGTGTCTGAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCNCGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGTAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACTTTGAATACGACACCCCGACCCGTCACGACGCACACGTAGACTGCCCTGGGc                                   <  2:743107/299‑1 (MQ=11)                                                                                                                                                                                  gATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTAAGCACACGTAGACTGCCCGGGGCa                                  >  1:413502/1‑300 (MQ=255)                                                                                                                                                                                                             tgtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTAACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCaa       >  1:163945/1‑299 (MQ=255)                                                                                                                                                                                                              gtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCAcc      >  1:527230/1‑300 (MQ=12)                                                                                                                                                                                                              gtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGCCCCGTCACTACGCACACGTAGACTGCACGGGGCACGCCGACTATGTTAAAAACATGATCAcc      >  2:227083/1‑300 (MQ=11)                                                                                                                                                                                                              gtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAATATGATCaac      >  1:444362/1‑298 (MQ=11)                                                                                                                                                                                                                  tAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCACGGGGCACGCCGACTATGTTAAAAACATGATCAACGGtg  >  1:229865/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                          CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCACCGGTG  >  NC_000913/4175721‑4176229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑