Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,172,010 C→T intergenic (+254/‑47) rrfB → / → murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,172,0100CT98.9% 292.6 / NA 87intergenic (+254/‑47)rrfB/murB5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (48/38);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACATGT  >  NC_000913/4171715‑4172304
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
gtatgGGGTCTCCCCATGCGAGAGGAGGGAACTGCCAGGCATCAAATAAAACTANAGACTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAATGCCATCCTGACGGATGGCNTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTcaca                                                                                                                                                                                                                                                                                                    <  2:790381/297‑1 (MQ=255)
  gtgGGGTCTCCCCATGCGAGAGGAGGGAACTGCCAGGCATCAAATAAAACGACAGGCTCAGTCGAAAGACTGTGCCATCCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGa                                                                                                                                                                                                                                                                                                  <  2:553387/300‑1 (MQ=255)
         ctcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTTAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTa                                                                                                                                                                                                                                                                                           >  1:832881/1‑300 (MQ=255)
           ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCACGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCACTCACCGATACGGTaaa                                                                                                                                                                                                                                                                                         >  1:181610/1‑300 (MQ=255)
           caccATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACCAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATCCGGCaaa                                                                                                                                                                                                                                                                                         >  2:364629/3‑300 (MQ=255)
             ccATGCGAGAGTATGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACt                                                                                                                                                                                                                                                                                       <  1:42513/300‑1 (MQ=255)
             ccATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCAAGGCATCAAATTACGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACt                                                                                                                                                                                                                                                                                       >  1:234798/1‑300 (MQ=255)
             acATGCGAGGGGAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACt                                                                                                                                                                                                                                                                                       <  2:284638/299‑1 (MQ=255)
                    agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCg                                                                                                                                                                                                                                                                                >  1:638032/1‑300 (MQ=255)
                                 gCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAgg                                                                                                                                                                                                                                                                   >  1:65440/1‑300 (MQ=255)
                                      gCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAagc                                                                                                                                                                                                                                                              >  2:531043/1‑300 (MQ=255)
                                                aaaCTAAAGGCTCCGTCGAAAGACTGGACCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAAc                                                                                                                                                                                                                                                    <  2:408984/300‑1 (MQ=255)
                                                aaaCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAAc                                                                                                                                                                                                                                                    <  1:65094/300‑1 (MQ=255)
                                                             aGTCGAAAGACTGGGCCCTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAAc                                                                                                                                                                                                                                       <  1:473511/300‑1 (MQ=255)
                                                              gTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGCACCCCTCCTTACAAcc                                                                                                                                                                                                                                      >  1:357923/1‑300 (MQ=255)
                                                               tCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAAccc                                                                                                                                                                                                                                     <  1:470049/300‑1 (MQ=255)
                                                                    aGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTACTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCATGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCCTCCCCTCACAGATACGGTCAACTCGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAAACCTGGaa                                                                                                                                                                                                                                >  2:417358/1‑300 (MQ=255)
                                                                     gACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAac                                                                                                                                                                                                                               >  2:374166/1‑300 (MQ=255)
                                                                                     ttATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCNTAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATc                                                                                                                                                                                                               <  1:617141/300‑1 (MQ=255)
                                                                                           gttgttTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGNACGTTGCGAAGCAACGGCCAGGAGGGTGGCGGGCAGGACGCCCGCCATAACCTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATGTCTACAAGCAGTCCCCTCACAGATACGGTAGACTAGCCTCGTTTTTGCATCAGGAAANTAGCTATGAACCAATCATTAAAACCCTGGAACACATTTGGCACTGATAATAATg                                                                                                                                                                                                         >  2:784081/1‑300 (MQ=21)
                                                                                            ttgtttgtCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGc                                                                                                                                                                                                        <  2:330875/300‑1 (MQ=255)
                                                                                                     ggTGAACGCTCTCATGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACAt                                                                                                                                                                                               >  2:330190/1‑300 (MQ=255)
                                                                                                        gAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGt                                                                                                                                                                                            >  1:826202/1‑300 (MQ=255)
                                                                                                           cGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTAtg                                                                                                                                                                                         <  1:183744/300‑1 (MQ=255)
                                                                                                            gctcttttgggggggacggatcaggtgggAGCGGATTTGAGCGTTGCGGAGTAGCGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAATGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAGACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATATGGTAAATTAGCCTCGTTTTTGTATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGTATTGATCATGATGTTCAGCACATTGTAtgt                                                                                                                                                                                        <  2:47984/274‑1 (MQ=25)
                                                                                                            gCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCATCGTTTTTGCCTCAGGAAAGCAGCTATGAACCCCTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTAtgt                                                                                                                                                                                        >  1:211055/1‑300 (MQ=255)
                                                                                                               ctcatGAGTAGGACAAATCCGCCGGGAGTGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCATAACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCATGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGcc                                                                                                                                                                                     >  2:275867/5‑300 (MQ=255)
                                                                                                               ctcatGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGcc                                                                                                                                                                                     >  2:60739/5‑300 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCCCATTGTATGTGcc                                                                                                                                                                                     >  2:24106/1‑300 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGcc                                                                                                                                                                                     >  1:387203/1‑300 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTACTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAACACCCTGGAACACATTTGGCATTGATCATACTGCTCCGCACATTGTATGTGcc                                                                                                                                                                                     >  2:527975/1‑300 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGAATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCAATCCTTAAAACCCTGGAACACATTTGGCATTGATTATAATGCTCAGCCTATTGTATGTGcc                                                                                                                                                                                     >  2:26161/1‑300 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCACGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGANGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCACTCNCAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCATGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGca                                                                                                                                                                                     >  2:670484/1‑299 (MQ=255)
                                                                                                               ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCACGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGcc                                                                                                                                                                                     >  2:371022/1‑300 (MQ=255)
                                                                                                                 ccTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGACGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTCAACTAGCCTCGTTTTTGCCTCAGGACAGCAGCTATGAACCACTCCTTAAAACCCCGGAACACGTTTGGCATTGATTATAATGCTCAGTACATTGTATGTGCCGa                                                                                                                                                                                   >  2:315094/1‑300 (MQ=25)
                                                                                                                    gAGTAGGACAAATCCGCAGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTAAAAGCCATCCCCTAACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCAATCATTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGagga                                                                                                                                                                                >  2:541137/1‑297 (MQ=255)
                                                                                                                          gACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATAAGGAAAGCAGCTATGAACCACTCCTTAAAACCATGGAAAACATTTGGCATTGATCATAATGCTAAGCACATTGTATGTGCCGAAGAAGaaca                                                                                                                                                                          >  2:71710/1‑300 (MQ=255)
                                                                                                                                          gCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCNTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGcc                                                                                                                                                          <  2:744269/300‑1 (MQ=255)
                                                                                                                                             gATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTgg                                                                                                                                                       >  2:511249/1‑300 (MQ=255)
                                                                                                                                                ttGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCTTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCGGGAACACAGGGGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAg                                                                                                                                                    <  2:85695/300‑1 (MQ=255)
                                                                                                                                                   aaCGTTGCGAAGCAAAGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAATGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTGGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAACTACTCAATGCCTGGCAGTAt                                                                                                                                                 <  2:8067/300‑1 (MQ=255)
                                                                                                                                                    aCGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATg                                                                                                                                                <  1:492978/300‑1 (MQ=255)
                                                                                                                                                     cGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCAGAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCGTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGc                                                                                                                                               <  2:327575/300‑1 (MQ=255)
                                                                                                                                                          cGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCg                                                                                                                                          <  1:283260/300‑1 (MQ=255)
                                                                                                                                                                aaCGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAANGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTNCTCAATGCCTGGCAGTATGCAACCGCAGAAg                                                                                                                                    <  2:610153/300‑1 (MQ=255)
                                                                                                                                                                   ggCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTANGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGAc                                                                                                                                 <  2:630148/300‑1 (MQ=255)
                                                                                                                                                                   ggCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCGGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGAc                                                                                                                                 <  1:254995/300‑1 (MQ=255)
                                                                                                                                                                    gCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACACACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACa                                                                                                                                >  1:346187/1‑300 (MQ=255)
                                                                                                                                                                                gCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTACTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTAGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCATTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTCTGTGCCGAAGACGAAAAACACTTACTAACTGCCTGGCAGTATGCAACCGTAGACGGACAACCCGTTCTTAt                                                                                                                    >  2:224828/1‑300 (MQ=38)
                                                                                                                                                                                  gggCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACATATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCGTGGCAGTATGCAAACGCAGAAGGACAACCCGTTCTTATTc                                                                                                                  >  2:728315/1‑300 (MQ=255)
                                                                                                                                                                                  gggCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc                                                                                                                  <  2:656699/300‑1 (MQ=255)
                                                                                                                                                                                  gggCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc                                                                                                                  >  1:55458/1‑300 (MQ=255)
                                                                                                                                                                                  gggCAGGACGCCCGCCATAAACTGCCAGGCAGCAAAGTAAGCAGAAGGCCACCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCAGCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc                                                                                                                  <  2:500159/300‑1 (MQ=255)
                                                                                                                                                                                   ggCAGAACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCACTCACCGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGAAGAAAAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAAACCGTTCTTATTCt                                                                                                                 >  1:193505/1‑300 (MQ=255)
                                                                                                                                                                                            cccgccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAAACCGTTCTTCTTCTGGGTgaagg                                                                                                        >  1:450740/1‑300 (MQ=255)
                                                                                                                                                                                             ccgccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaagga                                                                                                       <  2:477944/300‑1 (MQ=255)
                                                                                                                                                                                               gccATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTgaaggaag                                                                                                     >  1:258290/1‑300 (MQ=255)
                                                                                                                                                                                                       cTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCATGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTAc                                                                                             >  1:199754/1‑300 (MQ=255)
                                                                                                                                                                                                                     aTTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTTTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGAc                                                                               <  1:208587/300‑1 (MQ=255)
                                                                                                                                                                                                                     aTTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGAc                                                                               <  1:208427/300‑1 (MQ=255)
                                                                                                                                                                                                                               aGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCa                                                                     >  1:483855/1‑300 (MQ=255)
                                                                                                                                                                                                                                      ccTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACNATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTgct                                                              >  2:735478/1‑298 (MQ=255)
                                                                                                                                                                                                                                       cTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGGTCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACANTTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGatc                                                             >  2:742383/1‑300 (MQ=255)
                                                                                                                                                                                                                                          aCGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTCCAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAANCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGNCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAATGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGatcatc                                                          <  2:682601/300‑1 (MQ=255)
                                                                                                                                                                                                                                          aCGGATGGCCTTTTGGCGTTTCTACAAACTCTTCCTGTCGTCATATCGACAAGCCATCCCCTCACAGATACGGTAAACTATCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGatcatc                                                          <  2:135696/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGCGTTTCTACAAACTCTTCCTGTCTTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAACTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGTTGATCATCAACCGGATCAAAgg                                            <  2:79473/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAATCCATCCCCTCACAGATACGGTAAACCAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTGGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACAAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAgg                                            <  2:28661/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAgg                                            <  1:41011/300‑1 (MQ=255)
                                                                                                                                                                                                                                                             ttCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTGTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCg                                       <  2:709642/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ctctTCCTGTCGTCATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCatg                              >  2:490576/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                              gtcgtcATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAAACCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTATGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAAcat                      >  2:393568/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                cgtcATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGa                    >  1:304210/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                cgtcATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAACAGCTATGAACCACTCATTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAAACCGTTCTTATTCTGGGTGAAGGAAGTAAGGTACTCTTTCTGGAGGACTCTCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCACGATGAACCtaa                    >  2:329750/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                 gtcATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCCTCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGAGGCACGGTGATCATCAAACAGATCAAAGGTATCAAAATTCATGATGAACCTGAt                   >  2:96694/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                  tcATATCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATg                  <  1:230007/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      atCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTg              >  2:503348/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      atCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTg              >  1:793819/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      atCTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCNTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTg              >  2:733071/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                        cTACAAGCCATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGt            >  1:96314/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                               ccATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGTAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGANCAACAACTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGTTGAAGTAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACa     <  2:192502/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               ccATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTTGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACa     <  2:813099/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               ccATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAATTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACa     <  2:376128/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               ccATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACa     <  2:237757/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               ccATCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACa     <  1:541475/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 aTCCCCTCACAGATACGGTAAATTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACATg   >  1:412139/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  tCCCCTCACAGATACGGTCAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTACAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGNGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGCTGAACCTGATGCGTGGTATTTACATGt  >  1:567054/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  tCCCCTCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACATGt  <  1:79875/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
GTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAACCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCATAATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGGGTGAAGGAAGTAATGTACTTTTTCTGGAGGACTATCGCGGCACGGTGATCATCAACCGGATCAAAGGTATCGAAATTCATGATGAACCTGATGCGTGGTATTTACATGT  >  NC_000913/4171715‑4172304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑