Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 288,641 G→A A174A (GCC→GCT)  xynR ← DNA‑binding transcriptional repressor XynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913288,6410GA98.2% 183.0 / ‑3.8 55A174A (GCC→GCT) xynRDNA‑binding transcriptional repressor XynR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (30/24);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288378‑288934
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
ctTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTa                                                                                                                                                                                                                                                                   >  1:51624/1‑300 (MQ=255)
     ccTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTa                                                                                                                                                                                                                                                              >  1:64573/1‑300 (MQ=255)
      ctactaaacGGTCTAACGGCTCAGGCCGGGTAACCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCTTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCTTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTAc                                                                                                                                                                                                                                                             <  2:73955/292‑1 (MQ=255)
      cTACGATCCGGTCGAACGGCTCAGGCCTGTTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGTAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTAc                                                                                                                                                                                                                                                             <  2:411632/300‑1 (MQ=255)
           aGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATa                                                                                                                                                                                                                                                        <  1:847801/300‑1 (MQ=255)
             ccGGTCTAACAGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCAGAAATTAGCCGGCGCCGCGTCGTCCACGCGGGAGTTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGc                                                                                                                                                                                                                                                      <  2:60847/300‑1 (MQ=255)
                gTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCt                                                                                                                                                                                                                                                   >  1:104921/1‑300 (MQ=255)
                gTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCt                                                                                                                                                                                                                                                   >  2:343142/1‑300 (MQ=255)
                     aCGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCcagc                                                                                                                                                                                                                                              <  1:543449/300‑1 (MQ=255)
                       ggCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACNGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCcagcag                                                                                                                                                                                                                                            <  1:582551/300‑1 (MQ=255)
                       ggCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCcagcag                                                                                                                                                                                                                                            <  2:541947/300‑1 (MQ=255)
                        gCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAgg                                                                                                                                                                                                                                           >  1:653452/1‑300 (MQ=255)
                                cGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAg                                                                                                                                                                                                                                   <  2:501605/300‑1 (MQ=255)
                                             gcgcgcgAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGccc                                                                                                                                                                                                                      >  1:37928/1‑300 (MQ=255)
                                                              cccGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGNGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTANTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAacac                                                                                                                                                                                                     <  2:790821/300‑1 (MQ=255)
                                                                     tgctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAANGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCg                                                                                                                                                                                              >  2:732083/1‑300 (MQ=255)
                                                                      gctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGa                                                                                                                                                                                             >  1:643491/1‑300 (MQ=255)
                                                                      gctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGa                                                                                                                                                                                             >  2:294121/1‑300 (MQ=255)
                                                                          aaGCGGCTCGCGGAGATTAACCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAGCACCTTGCCGATGGc                                                                                                                                                                                         <  2:364817/300‑1 (MQ=255)
                                                                                      gAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCAc                                                                                                                                                                             <  1:556332/300‑1 (MQ=255)
                                                                                                 gCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCAAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAggcggc                                                                                                                                                                  >  2:551382/1‑300 (MQ=255)
                                                                                                           gtcCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCAAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGCAAAGGTCGNGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGACGATGCg                                                                                                                                                        >  2:731476/1‑300 (MQ=255)
                                                                                                                                             gcggcgATGACGCGGGACTCGGGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATATGCGATGGCGGCCAGCTTGCCTTCAAtc                                                                                                                      <  2:99238/300‑1 (MQ=255)
                                                                                                                                                gcgATGACGCGGGACTCGTGGTTCCAAACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAAtcttc                                                                                                                   >  2:1232/1‑300 (MQ=255)
                                                                                                                                                            gACTCGTGGTTCCACACCTGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGAcc                                                                                                       <  1:716695/300‑1 (MQ=255)
                                                                                                                                                            gACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGAcc                                                                                                       <  1:398205/300‑1 (MQ=255)
                                                                                                                                                            gACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGAcc                                                                                                       <  2:46146/300‑1 (MQ=255)
                                                                                                                                                            gACTCGTGGTTCCACACCGGCACCGCCACGCAACGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTNCTGATAGCCCTCCAGCAGGGCGTTCANCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGAcc                                                                                                       <  2:578667/300‑1 (MQ=255)
                                                                                                                                                              cTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGAcccc                                                                                                     <  1:824303/300‑1 (MQ=255)
                                                                                                                                                                    gTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGNGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCANGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGAGATGGCGGCCAGCTTGCCTTCAATCTTCTAGATATAGACCCCTTCACg                                                                                               >  2:675881/1‑300 (MQ=255)
                                                                                                                                                                      tCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCTGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGGTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCATGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCActcc                                                                                             <  1:750610/300‑4 (MQ=255)
                                                                                                                                                                      tCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGcc                                                                                             <  1:633360/300‑1 (MQ=255)
                                                                                                                                                                                  ccGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATcc                                                                                 >  1:518136/1‑300 (MQ=255)
                                                                                                                                                                                            gcgcACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAANCAACACCTTGCAGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGggt                                                                       >  2:198742/1‑300 (MQ=255)
                                                                                                                                                                                                     ctacTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCNGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGccc                                                              >  1:588128/4‑300 (MQ=255)
                                                                                                                                                                                                     cTGCTCATTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGccc                                                              <  1:157707/300‑1 (MQ=255)
                                                                                                                                                                                                                  tCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGAc                                                 <  1:109888/300‑1 (MQ=255)
                                                                                                                                                                                                                       ggaaaGGGCGTAGCCTTGCTAGCGGGTCTGCGCCAGGGTGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAGAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTTGGCCTCGCCCAGCCAGGCAATCAACACCTTGACGGTGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCCTGCCTTCAATCTTCTAGATATAAACCCATTCACGCCCGTCCGGGCTCCACAGCTGGGTGGCCTGCCCGGCCCGCGGGAACAGCTc                                            >  2:130825/5‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGGGGGTGGCGGGCGTAAAGGTAGTGTACTGCTAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGNATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGCTATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:741473/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTNCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCATTCAATCTTCTAGATATAGAACCATTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:201908/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGNGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  1:613822/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGNATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTAGATATAGACCCCTTAACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCAGGGACAGCTc                                            >  2:741479/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCGGCTTGGCTTCAATCTTCTCGATATAGACCCCTTNACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:619341/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTNACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGNCAGCTc                                            >  2:696999/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGCTGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:824833/1‑300 (MQ=255)
                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  1:45710/1‑300 (MQ=255)
                                                                                                                                                                                                                          cAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGt                                         <  2:464753/300‑1 (MQ=255)
                                                                                                                                                                                                                            gggCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTca                                       >  2:131692/1‑300 (MQ=255)
                                                                                                                                                                                                                              gCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTAGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTcagc                                     >  1:236639/1‑300 (MQ=255)
                                                                                                                                                                                                                                        gCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAGAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAACCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTACCTTCAATCTTCTCGATGTAGCCCCATTCACGCCCGTCTAGGATCCACAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTg                           >  2:343026/1‑300 (MQ=255)
                                                                                                                                                                                                                                         cTCGCGGGTCTGCGCCAGGGCACTTATTAAAACTTCACGAGACTCGAGGGTGGCGGGCGTAAAGGTAAGGTACCGGGAACCCTCCAGCAGGACGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGTGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGTCGGCAATCTTCTTGATATAGACCCCTTCACGCCCGTCCAGGACCCCCAGATGGGTGGTCTGCCCGGTCCGCCTGGACAGCTCCGTCAGCCAGCCTTTTGc                          <  2:86814/300‑1 (MQ=255)
                                                                                                                                                                                                                                                    gcgcCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCNATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTNGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCACGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGaa               >  2:672781/1‑300 (MQ=255)
                                                                                                                                                                                                                                                    gcgcCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGaa               >  1:246128/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       ccAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGTTAGTGTACTGATATCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTTAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAatat            <  2:549917/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGcc  >  2:322031/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                 cATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTATAGATATAGACCCATTCACGCCCGTCCAGGATCCACAGATGGGTGGTCTGCCCGGTCCGCAAGGATAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGcc  >  2:44118/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
CTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288378‑288934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑