Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	281,053	T→C	T311A (ACG→GCG)	yagA ←	integrase core domain‑containing protein YagA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,053	0	T	C	100.0%	188.8 / NA	53	T311A (ACG→GCG)	yagA	integrase core domain‑containing protein YagA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/32);  total (21/32)

GACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTG  >  NC_000913/280757‑281333                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         gaCCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCtgt                                                                                                                                                                                                                                                                                       >  1:500931/1‑300 (MQ=255)   ccATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTgtgt                                                                                                                                                                                                                                                                                     <  2:547729/300‑1 (MQ=255)      tGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGTGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGc                                                                                                                                                                                                                                                                                  <  2:77067/300‑1 (MQ=255)        ttCGGCGAAGGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGTGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCg                                                                                                                                                                                                                                                                                <  2:104177/300‑1 (MQ=255)                 tGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGAGCGGCTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGc                                                                                                                                                                                                                                                                       <  2:511919/300‑1 (MQ=255)                   agaATANTTGCTTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTNTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCcg                                                                                                                                                                                                                                                                     <  2:693352/300‑1 (MQ=255)                             gccgccgccGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCANACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGc                                                                                                                                                                                                                                                           >  1:569898/4‑300 (MQ=255)                                   gccgACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCNTGTTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATAc                                                                                                                                                                                                                                                     <  2:626058/300‑1 (MQ=255)                                                 ggtCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCTTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGTGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTAc                                                                                                                                                                                                                                       <  2:428748/300‑1 (MQ=255)                                                      gggggaTGGTGAGCACTTTTTAACATCCTTTACCCATGGTGATTGACTTTTTCTTCAGGTCGATCACCCCCAATTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTAAGCCAGAACCGTTCTCCCCTGAATGCATTGCCTGCGCCCAGACTTACCCCTTTCACGCTCAGATTTCCGCTGATGTCCACTTTCCCGACCACCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCGGTACTGCCGCGCTGACAGCTGacaacgagggtccggtagcgcaa                                                                                                                                                                                                                                  >  2:128860/6‑278 (MQ=255)                                                      gggACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCNTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCa                                                                                                                                                                                                                                  <  1:568463/300‑1 (MQ=255)                                                           aTGGTGAACACTTTTTAACATCCTTTACCCATGGTGAGCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATcc                                                                                                                                                                                                                             <  2:532400/300‑1 (MQ=255)                                                                gcacacTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcgc                                                                                                                                                                                                                        <  1:750151/298‑1 (MQ=255)                                                                gAACACTTTTTAACATCCTTTACCCGTGGTGAGCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCTTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcgc                                                                                                                                                                                                                        <  2:34994/300‑1 (MQ=255)                                                                gAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAgcgc                                                                                                                                                                                                                        <  2:406684/300‑1 (MQ=255)                                                                                  tgtACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGTGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATccctgtctcttatacacatctcgca                                                                                                                                                                                                      <  2:284926/298‑24 (MQ=255)                                                                                   ttACCCATGTTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCCTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAgg                                                                                                                                                                                                     <  2:433559/300‑1 (MQ=255)                                                                                         aTGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAg                                                                                                                                                                                               <  1:854594/300‑1 (MQ=255)                                                                                           gttGATCGACTTTTTCTTCAGTTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                                                                                                                             <  2:642555/298‑1 (MQ=255)                                                                                           ggTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGAc                                                                                                                                                                                             <  1:649698/300‑1 (MQ=255)                                                                                           ggTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTCCCTGCTGTACCGCCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCTGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAACGCCTCATGCGGGCGTTCAAGGTTATAGAc                                                                                                                                                                                             <  2:323644/300‑1 (MQ=255)                                                                                                                            cACTTTCGTGCTTTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCTACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGTGTGCGCTGTGGTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                                                                                                                            <  2:235753/300‑1 (MQ=255)                                                                                                                            cACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTTCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTACCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGTGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCATGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                                                                                                                            <  2:248905/300‑1 (MQ=255)                                                                                                                            cACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCGTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTc                                                                                                                                                            <  2:77479/300‑1 (MQ=255)                                                                                                                              cTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCAc                                                                                                                                                          >  1:837121/1‑300 (MQ=255)                                                                                                                                 tCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGc                                                                                                                                                       <  2:420335/300‑1 (MQ=255)                                                                                                                                 tCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGc                                                                                                                                                       <  1:693517/300‑1 (MQ=255)                                                                                                                                      cTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCt                                                                                                                                                  >  1:475086/1‑300 (MQ=255)                                                                                                                                        gTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCTGGTACCGCCATATCCAGCGCCTAGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGc                                                                                                                                                >  1:208883/1‑300 (MQ=255)                                                                                                                                        gTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGc                                                                                                                                                >  1:715761/1‑300 (MQ=255)                                                                                                                                           ccaccaCACCTCGTAGCTGCCGTCTTACTGCATCTCCTTCAGCCCGACCCGTTCTCCCATGAACGCCTTGCCTGCGCTCCGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGTTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCGGTGGTAGAAGGCGCGCTGCAGTTCAACGCTGTCTGCGaa                                                                                                                                             >  2:119403/1‑300 (MQ=255)                                                                                                                                             accacACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAAcc                                                                                                                                           >  1:243151/1‑300 (MQ=255)                                                                                                                                                   ccTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTc                                                                                                                                     >  1:153783/1‑300 (MQ=255)                                                                                                                                                     tCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTccc                                                                                                                                   >  1:548295/1‑300 (MQ=255)                                                                                                                                                     tCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCAGGGGGCGCTGTGTTGCCGCTGTACTGCTGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCATCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCAAACCATTTTccc                                                                                                                                   >  2:67792/1‑300 (MQ=255)                                                                                                                                                                              ccTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGc                                                                                                          >  1:454188/1‑300 (MQ=255)                                                                                                                                                                                 tCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCNGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCNATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGt                                                                                                       <  2:679471/300‑1 (MQ=255)                                                                                                                                                                                    gCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCATCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGaa                                                                                                    >  1:774071/1‑300 (MQ=255)                                                                                                                                                                                         aCCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGc                                                                                               >  1:245990/1‑300 (MQ=255)                                                                                                                                                                                             gTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCa                                                                                           >  1:747479/1‑300 (MQ=255)                                                                                                                                                                                             gTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTAGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCa                                                                                           >  1:50904/1‑300 (MQ=255)                                                                                                                                                                                                  ccccTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCATGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTc                                                                                      >  1:395404/1‑300 (MQ=255)                                                                                                                                                                                                     cTGAACNCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGNTATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTcccc                                                                                   <  2:701262/300‑1 (MQ=255)                                                                                                                                                                                                                gtcTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGTGGGCGCTGTGTTGCCGCTTTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCgg                                                                        <  2:284137/298‑1 (MQ=255)                                                                                                                                                                                                                      gcTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGCCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCACTGCGTCTGCGGATGATa                                                                  >  1:138504/1‑300 (MQ=255)                                                                                                                                                                                                                        tCAGACTTACCCCTTTCACGCTTAGCTTTCCGTTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAg                                                                <  2:99709/300‑1 (MQ=255)                                                                                                                                                                                                                          aGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGTGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGc                                                              <  1:828929/300‑1 (MQ=255)                                                                                                                                                                                                                                aCCCCTTTCACGCCCAGCTGTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCTTATCCCGGGTGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAg                                                        <  2:329301/300‑1 (MQ=255)                                                                                                                                                                                                                                        cACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCTTGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCAc                                                <  2:252837/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCACCACCCGGATACCCAGGCGCATCAGCCACAGc                    >  1:284212/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gACCATCACCCCTTCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAg               >  1:56337/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            cATCACCCCTTCATAGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGTTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAgcgc            <  2:245079/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tCATCGTATTCCGGGGGCGCTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTg  <  1:171577/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         GACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCACTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGCTGATATCCACTTTCCTGACCATCACCCCTTCATCGTATTCCGGGGGCGTTGTGTTGCCGCTGTACTGCCGCGCTGACGGCTGATACCGCGAGCCCGGTACCGCCATATCCAGCGCCTCGTGCGGGCGTTCAAGGTTATAGACCGTCCGCCAGTGGTCGAAGGCGCGCTGCAGTTCACCGCTGTCTGCGAACCATTTTCCCTGCAGCACTTCCGCCTTCAGGCTGCGGTGAAAACGCTCCAGCTTCCCCTGCGTCTGCGGATGATAAGGCCGGGAGTGCCCCACCCGGATACCCAGGCGCATCAGCCACAGCTCCAGCGCCGTCCAGGTG  >  NC_000913/280757‑281333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑