Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	274,985	G→A	P33S (CCA→TCA)	insH1 ←	IS5 family transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	274,985	0	G	A	100.0%	171.8 / NA	54	P33S (CCA→TCA)	insH1	IS5 family transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/22);  total (32/22)

TCGCGCCATCGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCG  >  NC_000913/274853‑275272                                                                                                                                     |                                                                                                                                                                                                                                                                                                tCGCGCCATCGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGa                                                                                                                          <  1:694481/300‑1 (MQ=14)   tgtataagagacAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAgg                                                                                                                        <  1:735001/289‑1 (MQ=9)   gcgcCATCGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGANTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAgg                                                                                                                        <  1:623936/300‑1 (MQ=14)   gcgcCATCGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAgg                                                                                                                        <  1:453114/300‑1 (MQ=14)          cGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGTAGGCGTTATg                                                                                                                 <  1:452994/300‑1 (MQ=17)          cGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATg                                                                                                                 >  1:80299/1‑300 (MQ=21)           gCTCAGGTTGTACCACTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCCTGGGGTAAAACGGCTCGATGACTTCCCACATGTTTTGCCCTGACAGAATCTGCTCCATGCGGGACACGCAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAATTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAACCATGATCCCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTCTGa                                                                                                                >  2:453486/1‑300 (MQ=11)           gCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGACGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGa                                                                                                                >  2:102885/1‑300 (MQ=21)             ttaGGTTGTACCAATGCTGCATGCAGTGAATTCGTAGCATGGTTTCCATCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCATGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGc                                                                                                              <  2:330596/298‑1 (MQ=255)             tCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGc                                                                                                              <  1:164921/300‑1 (MQ=25)               aGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTNGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTg                                                                                                            >  1:612973/1‑300 (MQ=25)                ggTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATTAGGGACTTGTTCGCACCTTCAGGAGGCGTTATGAGCTgg                                                                                                           >  2:445887/1‑300 (MQ=255)                ggTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTgg                                                                                                           >  2:423740/1‑300 (MQ=37)                ggTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTACGGAGGCGTTATGAGCTgg                                                                                                           >  2:257974/1‑300 (MQ=25)                ggTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTCTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTgg                                                                                                           >  1:789632/1‑300 (MQ=255)                 gTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATGAGGGACTTGTTCGCACCTTCCGGAGGctgtctcgtataca                                                                                                          >  2:719552/1‑287 (MQ=255)                    gTACCACTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCAACATGTTTTGCCATGACAGAATCTGCNCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCNGCGAAGGTAAGTTGATGACTCCTGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTAATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcggc                                                                                                       >  2:669213/1‑300 (MQ=255)                    gTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcggc                                                                                                       >  1:338172/1‑300 (MQ=255)                     tACCAATGCTGTATGCAGTGAATACGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATTTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGCATTCACTGTCGGCGCAGGTAAGTTGATGACTCATGATGAACCCCGCTCTATGGCTCCAGATGACGAACATGATCTCATATCGGGGACTTGTTCGCACCTTCAAGAAGCGTTATGAGCCggcggca                                                                                                      >  2:126510/1‑299 (MQ=11)                          aTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACTGCTCGATGACGTCCACCATGTTTTGCCATGACAGAATCTTCTCCATGCGGGACAAGAAAACCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCTTCGAAGGTAAGTTGACGACTCATGATGAACCCTGTTCTATTGCTCCAGACGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGTAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                 <  2:429561/300‑1 (MQ=11)                          aTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCGTGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                 >  1:177023/1‑300 (MQ=255)                          aTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACACACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                 >  1:225831/1‑300 (MQ=255)                          aTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                 >  1:177121/1‑300 (MQ=255)                          aTGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCtttt                                                                                                 >  2:815320/1‑300 (MQ=255)                            gctgcATGCAGTGAATGCGTAGCATGGTTTCNAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGNGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTg                                                                                               >  2:587961/1‑300 (MQ=255)                            gctgcATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGNATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCttttgg                                                                                               <  1:575126/300‑3 (MQ=255)                            gctgcATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTg                                                                                               <  1:646484/300‑1 (MQ=255)                              tgcatgcaGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTa                                                                                             >  1:187934/1‑300 (MQ=255)                              tgcatgcaGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTa                                                                                             >  2:821131/1‑300 (MQ=255)                               gcatgcaGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTTGCGGCCCTTTTTGTAt                                                                                            >  1:677746/1‑300 (MQ=255)                                    caGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACNCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGat                                                                                       >  1:603704/1‑300 (MQ=255)                                            gCGTAGCATGGTTTCCAGCGGATAAGGTCGCAGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAAGTAAGTTGACGACTCATGATGAACCATGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGTGGCCATTTTTGTATCTGATTATTAATc                                                                               >  2:245990/1‑300 (MQ=14)                                              gTAGCATGGTTTCCAGCGGATAAGGTCGCAGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACCAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTAATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGACTATTAATcca                                                                             >  2:422505/1‑299 (MQ=255)                                               tAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTTGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATcccc                                                                            <  2:173309/300‑1 (MQ=255)                                                     ggTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGc                                                                      >  1:393132/1‑300 (MQ=255)                                                      gTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCt                                                                     >  1:73020/1‑300 (MQ=255)                                                      gTTTCCAGCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAACTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCt                                                                     >  2:87735/1‑300 (MQ=255)                                                             gCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCGTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAgc                                                              <  1:267702/300‑1 (MQ=255)                                                             gCGGATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTNCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAgc                                                              <  1:575501/300‑1 (MQ=255)                                                               ggATAAGGTCGCCGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAgcgc                                                            >  1:631308/1‑300 (MQ=255)                                                                           cGGCCATTACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCa                                                <  1:514239/300‑1 (MQ=255)                                                                                 ttACCAACCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCg                                          >  2:319413/1‑300 (MQ=255)                                                                                    ccaaccTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCt                                       <  1:815976/296‑1 (MQ=255)                                                                                       accTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGt                                    >  1:58510/2‑300 (MQ=255)                                                                                        ccTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTg                                   >  1:559060/1‑300 (MQ=255)                                                                                              ggTAGAACGGCTCGATGACTTCCACCATGTTTTGTCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGt                             <  1:770010/300‑1 (MQ=255)                                                                                              ggTAAAACTGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGt                             <  1:237994/300‑1 (MQ=255)                                                                                              ggTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGt                             <  1:174211/300‑1 (MQ=255)                                                                                              ggTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTGATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTTTGGTGCAGGGt                             <  1:123750/300‑1 (MQ=255)                                                                                                   aaCGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACt                        <  1:403616/300‑1 (MQ=255)                                                                                                   aaCGGCTCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCGGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACt                        <  2:385718/300‑1 (MQ=255)                                                                                                         tCGATGACTTCCACCATGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCAt                  <  1:408468/300‑1 (MQ=255)                                                                                                                   ccaccaTGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACa        >  1:718774/1‑300 (MQ=255)                                                                                                                         tGTTTTGCCATGACAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAAcgcg  >  1:115003/1‑300 (MQ=255)                                                                                                                                     |                                                                                                                                                                                                                                                                                                TCGCGCCATCGCTCAGGTTGTACCAATGCTGCATGCAGTGAATGCGTAGCATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCG  >  NC_000913/274853‑275272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑