Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,862	C→T	A31A (GCC→GCT)	hyaD →	putative hydrogenase 1 maturation protease HyaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,862	0	C	T	100.0%	167.3 / NA	46	A31A (GCC→GCT)	hyaD	putative hydrogenase 1 maturation protease HyaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/18);  total (28/18)

TTTCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGAC  >  NC_000913/1035572‑1036153                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    tttCTGCTGGACGGGTGGCAACGCAATGGACATTCACAGCTTGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCAc                                                                                                                                                                                                                                                                                            <  2:409465/300‑1 (MQ=255) tttCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGTGNATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCAc                                                                                                                                                                                                                                                                                            <  2:493505/300‑1 (MQ=255)           aCGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAATACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGt                                                                                                                                                                                                                                                                                  <  2:499654/300‑1 (MQ=255)                 ggCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTgg                                                                                                                                                                                                                                                                            <  1:489387/300‑1 (MQ=255)                     aCTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGAt                                                                                                                                                                                                                                                                        >  1:568518/1‑300 (MQ=255)                             gACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGNTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATg                                                                                                                                                                                                                                                                >  2:158203/1‑300 (MQ=255)                                      aGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTc                                                                                                                                                                                                                                                       <  1:121721/300‑1 (MQ=255)                                            cATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGAc                                                                                                                                                                                                                                                 <  1:141071/300‑1 (MQ=255)                                                           tgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTATCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCTGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGt                                                                                                                                                                                                                                  <  2:341426/300‑1 (MQ=255)                                                               tGATTGGCGCGTTTGTGATCGTTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGt                                                                                                                                                                                                                              <  1:241650/300‑1 (MQ=255)                                                                     gcgcGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCACGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAg                                                                                                                                                                                                                        >  2:213462/1‑300 (MQ=255)                                                                        cgTTTGCGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGNCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGNGACTGAACTTGCTGGGGTATGTCGAAAgcgc                                                                                                                                                                                                                     <  2:471376/300‑1 (MQ=255)                                                                          tttGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCgcca                                                                                                                                                                                                                   >  1:248737/1‑300 (MQ=255)                                                                                    gTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGtt                                                                                                                                                                                                         >  2:593692/1‑300 (MQ=255)                                                                                      cATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCNGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGa                                                                                                                                                                                                       >  1:452592/1‑300 (MQ=255)                                                                                      cATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATAGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCNGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGa                                                                                                                                                                                                       >  1:452267/1‑300 (MQ=255)                                                                                        tGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATTTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGTGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGCGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCTAGCCATCTGTTGAtt                                                                                                                                                                                                     >  2:56887/1‑300 (MQ=255)                                                                                          tCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCANGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGNACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATtct                                                                                                                                                                                                   <  2:610828/300‑1 (MQ=255)                                                                                           cTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAAACTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATtctc                                                                                                                                                                                                  >  2:97245/1‑300 (MQ=255)                                                                                                       gCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCCCTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGAc                                                                                                                                                                                      >  1:620752/1‑300 (MQ=255)                                                                                                       gCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGAc                                                                                                                                                                                      >  2:414004/1‑300 (MQ=255)                                                                                                       gCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGAc                                                                                                                                                                                      >  1:620371/1‑300 (MQ=255)                                                                                                               aCATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTAACACTGGCACGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGct                                                                                                                                                                              >  1:640374/1‑300 (MQ=255)                                                                                                                           acgacACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctggaacctggaac                                                                                                                                                                  <  1:556644/300‑1 (MQ=255)                                                                                                                              acacGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATTGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCt                                                                                                                                                               >  1:422007/1‑300 (MQ=255)                                                                                                                              acacGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCt                                                                                                                                                               >  1:502808/1‑300 (MQ=255)                                                                                                                                     gATCTACACCATGGTCAACGGCTACCGTAGCCACAAATTTGGAAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCACCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAAcc                                                                                                                                                        >  1:40449/1‑300 (MQ=255)                                                                                                                                      aTCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCAATGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAACGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGCACCt                                                                                                                                                       >  2:171753/1‑300 (MQ=255)                                                                                                                                       tctcCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTa                                                                                                                                                      >  2:316022/1‑300 (MQ=255)                                                                                                                                                                 aGCCACAAATTTGGCAAAATAAGTAACAATGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGTGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtc                                                                                                                            <  2:329667/300‑1 (MQ=255)                                                                                                                                                                   ccACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGATGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                                                                                                                          <  1:431264/300‑1 (MQ=255)                                                                                                                                                                   ccACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGNGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctc                                                                                                                          <  1:455466/300‑1 (MQ=255)                                                                                                                                                                                    tAAGTAACAAGGAGCGTTCATGAGCGAGTAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAg                                                                                                         <  1:50848/300‑1 (MQ=255)                                                                                                                                                                                     aaGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGc                                                                                                        >  1:389751/1‑300 (MQ=255)                                                                                                                                                                                                 gCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAAc                                                                                            <  1:127758/300‑1 (MQ=255)                                                                                                                                                                                                   gTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCATGCATCAGAACAg                                                                                          >  2:385776/1‑300 (MQ=255)                                                                                                                                                                                                                           ggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACTTATGCCGGAGAACGCATTCCGGCTTATCTCCGCGCGACGAAAATGAGCCTGCATCAGAACAGTTTCTACGAAGTgttggcgttggc                                                                  >  2:208538/1‑300 (MQ=255)                                                                                                                                                                                                                                 ggggCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGatat                                                            <  1:556744/300‑1 (MQ=255)                                                                                                                                                                                                                                              ctgctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTATCCAAAGTGTTGGTGTTGGCGGATATCCGCGGACATCTg                                               >  2:124483/1‑300 (MQ=255)                                                                                                                                                                                                                                                gctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGcc                                             >  1:357565/1‑300 (MQ=255)                                                                                                                                                                                                                                                gctgTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTAAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTACGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGcc                                             >  2:365738/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ttCGGCGTGCGGGTTGCGGGACGGCTGTATGCTCACTACCACTGGCCCGAGTATTTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCTAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCtcgtc                          <  2:209292/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   cGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTCCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCaa          >  1:382106/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcga   >  1:491006/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTAAGGGCTGGAACCTGGAACGCTGAGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTACGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcga   >  2:328123/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           cTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTAAGCGCGAAGAAAATNAGCCTGCATCAGAACAGTTTCTACGAAGTGTTGGCGTTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTcgac  >  2:588810/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    TTTCTACTGGACGGGTGGCAACTCAATGGACATTCACAGCTGGCATCGGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGAC  >  NC_000913/1035572‑1036153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑