Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	977,287	G→A	G321D (GGT→GAT)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	977,287	0	G	A	100.0%	158.9 / NA	46	G321D (GGT→GAT)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/21);  total (25/21)

AACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCG  >  NC_000913/976998‑977582                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        aaCAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACGGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGc                                                                                                                                                                                                                                                                                               <  1:658058/300‑1 (MQ=255)                      ttCCCGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTAACCCAGTCGGACCGCGAACTGTTTCAGCATCTGATCAGCGAAGCAACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTAGACACCGCACTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGTACAACGATGCCGACGGCGATCTGGAAACGGatcatcca                                                                                                                                                                                                                                                                          >  2:339402/1‑298 (MQ=255)                                 ggAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGa                                                                                                                                                                                                                                                               <  2:88678/300‑1 (MQ=255)                                   aaGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATc                                                                                                                                                                                                                                                             >  1:532997/1‑300 (MQ=255)                                           cTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAAc                                                                                                                                                                                                                                                     >  2:131839/1‑300 (MQ=255)                                             gCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGCTCACCTGAACCt                                                                                                                                                                                                                                                   >  1:603553/1‑300 (MQ=255)                                             gCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCt                                                                                                                                                                                                                                                   <  2:126876/300‑1 (MQ=255)                                             gCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCNACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATTAGGCGGCCAGTGATCACCTGAACCt                                                                                                                                                                                                                                                   >  2:152902/1‑300 (MQ=255)                                                       cGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAAcc                                                                                                                                                                                                                                         <  2:220105/300‑1 (MQ=255)                                                          aTGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCa                                                                                                                                                                                                                                      >  1:273306/1‑300 (MQ=255)                                                           tGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCAc                                                                                                                                                                                                                                     <  1:347295/300‑1 (MQ=255)                                                                             gtgtCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCNCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGagaaga                                                                                                                                                                                                                   >  1:461810/1‑300 (MQ=255)                                                                              tgtCACCCAGTCGGACCGCGACCTGTGTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGANCTACATACTTCGCGTTAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGAt                                                                                                                                                                                                                  <  2:156363/300‑1 (MQ=255)                                                                                           gACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGaa                                                                                                                                                                                                     >  1:547832/1‑300 (MQ=255)                                                                                                      gTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCTCGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGAtctaga                                                                                                                                                                                          >  2:414334/1‑297 (MQ=255)                                                                                                        ttAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATg                                                                                                                                                                                        <  1:655397/300‑1 (MQ=255)                                                                                                             cATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTg                                                                                                                                                                                   >  1:605058/1‑300 (MQ=255)                                                                                                             cATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTAGATGAGCTg                                                                                                                                                                                   >  2:200010/1‑300 (MQ=255)                                                                                                               tCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCa                                                                                                                                                                                 >  1:649310/1‑300 (MQ=255)                                                                                                                  gATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGNGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGAt                                                                                                                                                                              <  1:464658/300‑1 (MQ=255)                                                                                                                             ccaccaACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAag                                                                                                                                                                   <  2:383801/300‑1 (MQ=255)                                                                                                                             ccaccaACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAag                                                                                                                                                                   <  1:579629/300‑1 (MQ=255)                                                                                                                              caccaACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAaga                                                                                                                                                                  >  1:595486/1‑300 (MQ=255)                                                                                                                                                 gACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCa                                                                                                                                               >  1:277248/1‑300 (MQ=255)                                                                                                                                                 gACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTAGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCa                                                                                                                                               >  2:555018/1‑300 (MQ=255)                                                                                                                                                       aTGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACCGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGcc                                                                                                                                         <  2:570361/300‑1 (MQ=255)                                                                                                                                                                         cgcCGTGTCCATCTCGACAAAGCCCTGTAGTTTCGGTGCGAGCTACATACGTCGCGGCAGCAACTGGCGGCTGAGCAGTACAAACACTGCGATATGGCGCGTGAGCGGTCAGAGCACACCGATGCCGAAGGCGAGTTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCTTCAGCAGTAGAAAATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGTAAGAGCAAAATGAGGTGTTGGTAGAAGCCATCGAACGCCAGCAagag                                                                                                                       <  2:314022/300‑1 (MQ=255)                                                                                                                                                                                  cATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCt                                                                                                              <  1:600625/300‑1 (MQ=255)                                                                                                                                                                                  cATCTAGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGGAGCCATCGAACGCCAGAAGGGGAATGAGGCt                                                                                                              >  2:242994/1‑300 (MQ=255)                                                                                                                                                                                      tcGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTg                                                                                                          <  2:207545/300‑1 (MQ=255)                                                                                                                                                                                              gCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCANTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAAACTGGTGCCAACCGCACTGCGTCAGCAGGAGAAGATCGACCGCTACGAAGCGGATCTAGATGAGCTGCAGATCCGTCGGGAAGAGCAAAATGANGTGGTGGCAGAAGCAATCGAACGACAGCAAGAGAATGAGGCTCGTGCGGAAGCt                                                                                                  >  2:608840/1‑300 (MQ=255)                                                                                                                                                                                              gCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCAGAAGGCGATCTGGAAGCGGATTATCAAGAGGCCAGCGATCACCTGAACCTGGTGCAAACCACACTGCGTCAGCAAGAGAAGATCGAATGCTACGAAGCGGATCTCGATGAGCTGCAGACCCGTCTGGAAGAGAAAAATGAAGTGGTGGCAGAGGCCATCGAACGCAAGCAAGAGAATGGGGTTTGTGCGAAAGcg                                                                                                  >  2:310966/1‑299 (MQ=255)                                                                                                                                                                                                                  gCTACATACTTCGCGTCAGCAACTGGCGGCTNAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATNACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGa                                                                              >  2:467032/1‑300 (MQ=255)                                                                                                                                                                                                                                       aCTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGa                                                         <  1:367273/300‑1 (MQ=255)                                                                                                                                                                                                                                                tGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACcagca                                                <  1:351046/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCt                                          >  1:68745/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCt                                          >  2:640243/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCAAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCt                                          >  1:258375/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cgcgTGAGCTGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTATGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAAcgcgcg                      >  2:54086/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tGGCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGt             <  2:275279/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gCAGAGCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGtat           <  1:76779/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gagCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGNGCTGCAGATCCGTCTGGAAGAGCAAANTGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAAc        >  2:449447/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gagCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAACTTGCTGACTACCAGTAGGCGCTAGACGTCCAGCAAACGCGCGCGATCCAGTATAAc        >  2:23593/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         agCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGANGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAAcc       <  1:457351/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gCACAACGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCa      <  1:567436/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              aaCGATGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCg  <  1:611341/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        AACAGCGGCGTGCGTAAAGCGTTCCAGGACATGGAAGCGGCGCTGCGTGAAAACCGTATGACGCTGGAAGCGATTCGTGTCACCCAGTCGGACCGCGACCTGTTTAAGCATCTGATCAGCGAAGCCACCAACTACGTGGCGGCGGACTACATGCGTCACGCCAACGAGCGCCGTGTCCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATGAGGCTCGTGCGGAAGCTGCCGAACTGGAAGTGGACGAGCTGAAAAGCCAGCTTGCTGACTACCAGCAGGCGCTGGACGTCCAGCAAACGCGCGCGATCCAGTATAACCAGGCG  >  NC_000913/976998‑977582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑