Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,038	G→A	H97Y (CAT→TAT)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,038	0	G	A	100.0%	163.4 / NA	48	H97Y (CAT→TAT)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/20);  total (28/20)

CGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACT  >  NC_000913/699754‑700331                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      cgcCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTANGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTc                                                                                                                                                                                                                                                                                        >  1:448525/1‑300 (MQ=255)             gTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAGTCACGAAATCAGGGTTCACATCGGTAANAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATa                                                                                                                                                                                                                                                                            >  2:482187/1‑300 (MQ=255)             gTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACAGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTCTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAAGGTTCACATAGGTAATAGTGAAACCGGATCTGTACAGTTCATAAATCAGTGCGCCTCCGCCCACCACATa                                                                                                                                                                                                                                                                            >  2:53372/1‑300 (MQ=255)               ttAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAANACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACg                                                                                                                                                                                                                                                                          <  2:580577/300‑1 (MQ=255)                        cgcgGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGNGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGc                                                                                                                                                                                                                                                                 <  1:464558/300‑1 (MQ=255)                            gatgatCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCACGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTc                                                                                                                                                                                                                                                             >  2:209473/1‑300 (MQ=255)                                 tCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCAAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGc                                                                                                                                                                                                                                                        >  1:559533/1‑300 (MQ=255)                                 tCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCAAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGc                                                                                                                                                                                                                                                        >  2:425259/1‑300 (MQ=255)                                              ctTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCCTGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCANTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCANGAAATc                                                                                                                                                                                                                                           >  2:549319/1‑300 (MQ=255)                                                tACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACCCGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTAGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTAGCCAACACTCACGAAATCAGGATTCACCTCGGTAATAGTGAAAACGGCTCTGTACAGTTCATAAATCAGTGCGCATTCGCCTACCACATATGCTTTCTTACCTTCCTGGCGACGAAGGAAATCga                                                                                                                                                                                                                                         >  2:265511/1‑299 (MQ=255)                                                tACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATCCGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCgg                                                                                                                                                                                                                                         >  2:409680/1‑300 (MQ=255)                                                                  tGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGNGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑GCGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAgg                                                                                                                                                                                                                       <  2:573958/300‑1 (MQ=255)                                                                       ccGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATaa                                                                                                                                                                                                                  <  1:633996/300‑1 (MQ=255)                                                                          gagaTTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAac                                                                                                                                                                                                               <  2:422086/300‑1 (MQ=255)                                                                                      aTCCCTGCACACAACGCGCCACAGGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGt                                                                                                                                                                                                   <  2:593828/300‑1 (MQ=255)                                                                                       tCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATACCTCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTa                                                                                                                                                                                                  >  2:352520/1‑300 (MQ=255)                                                                                       tCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTa                                                                                                                                                                                                  >  1:219181/1‑300 (MQ=255)                                                                                        cccTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGTCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTAc                                                                                                                                                                                                 <  1:571370/300‑1 (MQ=255)                                                                                         ccTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGGAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACa                                                                                                                                                                                                >  2:569227/1‑300 (MQ=255)                                                                                         ccTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACa                                                                                                                                                                                                >  1:40103/1‑300 (MQ=255)                                                                                          cTGCACNCAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATNGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACAt                                                                                                                                                                                               <  2:538232/300‑1 (MQ=255)                                                                                                                 ggATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTGGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCgg                                                                                                                                                                        >  2:601829/1‑300 (MQ=255)                                                                                                                                      gtgtCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACGTCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTc                                                                                                                                                   <  2:370633/300‑1 (MQ=255)                                                                                                                                            ggATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCNTCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCATAGGTATAAAACACGCTGTCANGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGaa                                                                                                                                             <  2:610745/300‑1 (MQ=255)                                                                                                                                            ggATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGaa                                                                                                                                             <  1:619110/300‑1 (MQ=255)                                                                                                                                                        aTAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCAAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCNGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATANTTGGTc                                                                                                                                 >  2:546454/1‑300 (MQ=255)                                                                                                                                                            aaCGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCa                                                                                                                             >  1:404669/1‑300 (MQ=255)                                                                                                                                                                            gacgaAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGcc                                                                                                             <  1:421813/300‑1 (MQ=255)                                                                                                                                                                               gacaTAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTATATCGACAACTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                                                                          >  1:616037/4‑300 (MQ=255)                                                                                                                                                                                        tgctTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCNCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCNGCAACACCAGCGGCAGGCCTTTATCCATAAt                                                                                                 <  2:478020/300‑1 (MQ=255)                                                                                                                                                                                          ctTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATcc                                                                                               >  1:186390/1‑300 (MQ=255)                                                                                                                                                                                          ctTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATcc                                                                                               >  1:186393/1‑300 (MQ=255)                                                                                                                                                                                           tttATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACACACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCCTAATccc                                                                                              >  2:378951/1‑300 (MQ=255)                                                                                                                                                                                           tttATGCATCATGTCCCAGTTGT‑GGAGACGTGTTTTGCCAACAATCACGAAATCAGGGTTCACATCGGGAATAGTGAAACCGGCTTTGTACAGTACATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCGTCCTGGCGACGCAGGAAATCTGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAAATCTTGCCCAGTCTGCTAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATccc                                                                                              <  2:205072/300‑1 (MQ=255)                                                                                                                                                                                                        tCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACTAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTcc                                                                                 >  1:219262/1‑300 (MQ=255)                                                                                                                                                                                                                                gCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGtt                                                          >  1:581931/1‑300 (MQ=255)                                                                                                                                                                                                                                    acaaTCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCg                                                      <  1:31390/300‑1 (MQ=255)                                                                                                                                                                                                                                         cACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCAt                                                 <  1:263925/300‑1 (MQ=255)                                                                                                                                                                                                                                             aaaTCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCAGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGc                                             >  2:408058/1‑300 (MQ=255)                                                                                                                                                                                                                                               aTCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCAc                                           <  1:613153/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gggTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCg                                       >  2:234170/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCATGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATg            >  1:296129/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gCTTTGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATg            >  1:37158/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                tttGTACAGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGAGCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGNGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTNAATGGt          >  2:549978/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       aGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGAc   <  1:519004/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTACTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGAc   <  1:558063/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aGTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTGGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGAc   <  2:502342/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gTTCATAAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACt  >  1:591546/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      CGCCTGCATTTTGTTTAATGCTGCGCGGATGATCCACGGGCTGGGCTTACCAACATAGAACGGTTTGCGCCCGGAGATTTTCTCAATCCCTGCACACAACGCGCCACAAGCGGGATAAAAACCGCGCCCGTGGGTGTCCGGATTGGTGGCGATAAAACGTGCACCGTTAGCGACGAAATAGGCTGCTTTATGCATCATGTCCCAGTTGTAGGA‑ACGCGTTTCGCCAACAATCACGAAATCAGGGTTCACATCGGTAATAGTGAAACCGGCTTTGTACAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACT  >  NC_000913/699754‑700331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑