Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	598,429	C→T	L317L (CTC→CTT)	cusB →	copper/silver export system membrane fusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	598,429	0	C	T	100.0%	199.6 / NA	55	L317L (CTC→CTT)	cusB	copper/silver export system membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/20);  total (35/20)

CGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAAT  >  NC_000913/598137‑598723                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       cgTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGAACGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCAGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCg                                                                                                                                                                                                                                                                                                 >  2:412308/1‑300 (MQ=255)  gTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTAGCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGc                                                                                                                                                                                                                                                                                                >  1:376356/1‑300 (MQ=255)   tttGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGAACGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGAACCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACAGGGCATGAACGCCTGGTTGCAACTTAACACCgac                                                                                                                                                                                                                                                                                               >  1:166170/1‑298 (MQ=255)                                  aaGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGCGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGAACGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGAACCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAAACGGGCATGAACGCCTGGTTACAACTTAACACCGCCAGCGAACCGATGCTACTAATTCCGCCACAAg                                                                                                                                                                                                                                                                >  2:239396/1‑300 (MQ=255)                                  aaGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTAACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCAGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGACAGCGAACCGATGATGCTCATTCCGTAACAAg                                                                                                                                                                                                                                                                >  2:239438/1‑300 (MQ=255)                                                        tcggggTATGGACCCGTTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGa                                                                                                                                                                                                                                          <  2:81746/297‑1 (MQ=255)                                                        tCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGa                                                                                                                                                                                                                                          <  1:411068/300‑1 (MQ=255)                                                          aGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAAc                                                                                                                                                                                                                                        <  1:660045/300‑1 (MQ=255)                                                            ggTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAg                                                                                                                                                                                                                                      >  1:612503/1‑300 (MQ=255)                                                             gTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGc                                                                                                                                                                                                                                     >  1:549813/1‑300 (MQ=255)                                                             gTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTATATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGAAACTTAACACCGCAAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGc                                                                                                                                                                                                                                     >  2:66022/1‑300 (MQ=255)                                                                  gACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAGCACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACAGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCAAAAGCGCTGATTGATACCGGTAGCGAACAGCGGGTg                                                                                                                                                                                                                                >  2:313385/1‑300 (MQ=255)                                                                      cGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTa                                                                                                                                                                                                                            >  1:132152/1‑300 (MQ=255)                                                                       ggTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGTGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGAAGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCACGCGAACCGATGCTGCTCATTCCGTCACCAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTAc                                                                                                                                                                                                                           >  2:11960/1‑300 (MQ=255)                                                                        gtgtGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATAACGGCAGCGAACAGCGGGTGATTAcc                                                                                                                                                                                                                          >  1:244018/1‑300 (MQ=255)                                                                          gtgGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGt                                                                                                                                                                                                                        <  2:316464/300‑1 (MQ=255)                                                                             ggTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCAGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGa                                                                                                                                                                                                                     >  1:179949/1‑300 (MQ=255)                                                                               tCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGTGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATg                                                                                                                                                                                                                   >  1:156203/1‑300 (MQ=255)                                                                                          aTCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGCACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGCCAGCGAACAGCGGGTGATTACCGTTGATGCCGGCGGgcgc                                                                                                                                                                                                        >  2:568489/1‑300 (MQ=255)                                                                                                  gTCTATCGCCTGGCTGGTGAAAGATGCCTCGNAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGNTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTAcc                                                                                                                                                                                                >  2:477267/1‑300 (MQ=255)                                                                                                  gTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTAcc                                                                                                                                                                                                >  2:253948/1‑300 (MQ=255)                                                                                                    cTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGa                                                                                                                                                                                              <  2:562011/300‑1 (MQ=255)                                                                                                      aTCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGNGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGaaa                                                                                                                                                                                            <  1:436613/300‑1 (MQ=255)                                                                                                        cGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGTGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAAcg                                                                                                                                                                                          <  2:222008/300‑1 (MQ=255)                                                                                                                          tGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGTGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGc                                                                                                                                                                        <  1:260956/300‑1 (MQ=255)                                                                                                                          tGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGc                                                                                                                                                                        <  2:200876/300‑1 (MQ=255)                                                                                                                                       aCCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTc                                                                                                                                                           >  1:368753/1‑300 (MQ=255)                                                                                                                                            cACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCANAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGc                                                                                                                                                      >  1:485064/1‑300 (MQ=255)                                                                                                                                                gTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTa                                                                                                                                                  >  1:252649/1‑300 (MQ=255)                                                                                                                                                   ccGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGc                                                                                                                                               <  2:129276/300‑1 (MQ=255)                                                                                                                                                          gACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCtctggtc                                                                                                                                        <  1:195796/300‑1 (MQ=255)                                                                                                                                                              ggATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCNGGTTGCAACTTAACACCGCCAGCGAANCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGc                                                                                                                                    >  2:440270/1‑300 (MQ=255)                                                                                                                                                                                    aaaTGGACGCTGCTACCTGGCGGGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTACAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGCCACAAGCGCTGATTGATACCGACAGCGAACAGCGGGTGATTACCGTTGATGCCGACGTGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAGAAGGTGGTTTcc                                                                                                              <  2:314168/300‑1 (MQ=255)                                                                                                                                                                                       tGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGc                                                                                                           >  1:393624/1‑300 (MQ=255)                                                                                                                                                                                             ctgctACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGAGAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTg                                                                                                     >  2:514511/1‑300 (MQ=255)                                                                                                                                                                                             ctgctACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTg                                                                                                     >  1:214640/1‑300 (MQ=255)                                                                                                                                                                                             ctgctACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTg                                                                                                     >  2:378326/1‑300 (MQ=255)                                                                                                                                                                                             ctgctACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCAAGCGAACCGANGCTGCTCATTCCGTCACAAGCGCTGANTGCTACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCAACGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTg                                                                                                     >  2:446032/1‑300 (MQ=255)                                                                                                                                                                                                ctACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACTAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTc                                                                                                  <  2:196084/300‑1 (MQ=255)                                                                                                                                                                                                 tACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTcc                                                                                                 <  1:281820/300‑1 (MQ=255)                                                                                                                                                                                                     tGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCtgat                                                                                             <  1:523329/300‑1 (MQ=255)                                                                                                                                                                                                      ggCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCtgatt                                                                                            >  1:40832/1‑300 (MQ=255)                                                                                                                                                                                                                ccGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAg                                                                                  >  1:361427/1‑300 (MQ=255)                                                                                                                                                                                                                     aCCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCNGATTGATACCGGCAGCGAACAGCGGGNGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGACAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAat                                                                             >  2:445467/1‑300 (MQ=255)                                                                                                                                                                                                                         gCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTNGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATAttt                                                                         >  1:461633/1‑300 (MQ=255)                                                                                                                                                                                                                              cTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGgc                                                                    >  1:151457/1‑300 (MQ=255)                                                                                                                                                                                                                              cTGCAGCTGCGTCTGGAAGTCGACAACGCCGACCAGGCGCTAAAACCGGGCATGAANGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGANAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTggt                                                                    >  2:614613/1‑299 (MQ=255)                                                                                                                                                                                                                                       cGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAg                                                           <  1:405098/300‑1 (MQ=255)                                                                                                                                                                                                                                                       aCGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGaaa                                           <  1:566314/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTAccc                                  <  1:523746/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     cTAAAACCGGGCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATgcg                             <  1:618493/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaaCCGGGCATGAACGCCTGGTGGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGCGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGCGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCAt                          <  2:178445/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              ggCATGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAggg                    >  2:13128/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  tGAACGCCTGGTTGCAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATa                >  1:613941/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cAACTTAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGAAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAAt  >  1:575507/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       CGTTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGTATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGATGCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAATGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCTGAAAGTGCTACCCATGCGCATTGAGGGAATAACCAATGATTGAAT  >  NC_000913/598137‑598723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑