Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,516	A→C	Y166* (TAT→TAG)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,516	0	A	C	100.0%	202.3 / NA	53	Y166* (TAT→TAG)	tsx	nucleoside‑specific channel‑forming protein Tsx
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/30);  total (23/30)

GACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAG  >  NC_000913/431227‑431801                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                              gACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCCGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCa                                                                                                                                                                                                                                                                                     >  1:300443/1‑300 (MQ=255) gACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCAAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAAACGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCAACTGGTATTTAGCCTAGACGTTCa                                                                                                                                                                                                                                                                                     >  1:586503/1‑300 (MQ=255)      ccGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGAc                                                                                                                                                                                                                                                                                <  2:342020/300‑1 (MQ=255)      ccGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGCTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCNTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCANCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGAc                                                                                                                                                                                                                                                                                <  2:535023/300‑1 (MQ=255)                  tAACGAGCTACTACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGc                                                                                                                                                                                                                                                                    <  2:254603/300‑1 (MQ=255)                  tAACGAGCTACGACAGATTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGNTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTNCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGc                                                                                                                                                                                                                                                                    <  2:622422/300‑1 (MQ=255)                    aCGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAg                                                                                                                                                                                                                                                                  <  1:607139/300‑1 (MQ=255)                       aGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGcc                                                                                                                                                                                                                                                               >  1:189704/1‑300 (MQ=255)                       aGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGcc                                                                                                                                                                                                                                                               >  2:220801/1‑300 (MQ=255)                            ctacatagtagtGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGTAAGCGATAGAGTTATTAGTCCGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGAAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGCTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCTTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAgtgt                                                                                                                                                                                                                                                          <  2:249920/294‑1 (MQ=255)                            cGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAgtgt                                                                                                                                                                                                                                                          >  1:659304/1‑300 (MQ=255)                                 gagTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCAACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTAATGGACAGGCTCATCGGCAGGCCAGTGTCgcta                                                                                                                                                                                                                                                     >  2:641382/1‑297 (MQ=255)                                               aTCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGAcc                                                                                                                                                                                                                                       <  2:138335/300‑1 (MQ=255)                                                      ttCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTAc                                                                                                                                                                                                                                <  1:11716/300‑1 (MQ=255)                                                       tCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTAcc                                                                                                                                                                                                                               <  2:356065/300‑1 (MQ=255)                                                            gCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTg                                                                                                                                                                                                                          <  1:593324/300‑1 (MQ=255)                                                                  atatGGCTGGAAGCGATAGAATTATTAGTACGGGTCTTAATACCGGTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTgg                                                                                                                                                                                                                    <  1:174467/300‑1 (MQ=255)                                                                                 aTAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTttatta                                                                                                                                                                                                     <  1:391803/300‑1 (MQ=255)                                                                                      gTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAANTCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGAcc                                                                                                                                                                                                <  2:496849/300‑1 (MQ=255)                                                                                      gTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGAcc                                                                                                                                                                                                <  2:412903/300‑1 (MQ=255)                                                                                                  ggTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAAt                                                                                                                                                                                    >  1:159605/1‑300 (MQ=255)                                                                                                   gTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGCCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATg                                                                                                                                                                                   <  2:626772/300‑1 (MQ=255)                                                                                                    tCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGCAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGt                                                                                                                                                                                  >  2:325215/1‑300 (MQ=255)                                                                                                    tCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGAACCATGTCGTAAATGt                                                                                                                                                                                  >  2:115120/1‑300 (MQ=255)                                                                                                                                 tCATCCNCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCNGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAAc                                                                                                                                                     <  2:545109/300‑1 (MQ=255)                                                                                                                                 tCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTTAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAAc                                                                                                                                                     <  1:330176/300‑1 (MQ=255)                                                                                                                                      cccTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGAcc                                                                                                                                                >  1:561252/1‑300 (MQ=255)                                                                                                                                                gAACCCCATTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCTGCACAAAGTATTTAATTTTGAAGCGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATGTCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAgg                                                                                                                                      <  1:119108/300‑1 (MQ=255)                                                                                                                                                gAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTGAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAgg                                                                                                                                      <  1:234024/300‑1 (MQ=255)                                                                                                                                                 aacctcattCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCTGCACAAAGTATTTAATTGTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGTCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGt                                                                                                                                     <  2:241318/292‑1 (MQ=255)                                                                                                                                                  aCCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTc                                                                                                                                    <  1:132161/300‑1 (MQ=255)                                                                                                                                                       aGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTAt                                                                                                                               <  1:187910/300‑1 (MQ=255)                                                                                                                                                        gTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTAtt                                                                                                                              >  1:82018/1‑300 (MQ=255)                                                                                                                                                          cGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTgg                                                                                                                            <  1:349716/300‑1 (MQ=255)                                                                                                                                                            aaGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTc                                                                                                                          <  1:34573/300‑1 (MQ=255)                                                                                                                                                            aaGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCACGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTc                                                                                                                          <  2:560238/300‑1 (MQ=255)                                                                                                                                                                           aTGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGCCGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGCCCGAAGCTAAGGTCAGTATTGGTCAGTTTGTCGATGgag                                                                                                           >  2:285344/1‑300 (MQ=255)                                                                                                                                                                              tAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGaaa                                                                                                        >  1:500037/1‑300 (MQ=255)                                                                                                                                                                                   cAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTgg                                                                                                   >  1:42557/1‑300 (MQ=255)                                                                                                                                                                                          ccGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATAGAGAAATGTGGTTCGAtt                                                                                            >  2:219751/1‑300 (MQ=255)                                                                                                                                                                                             ccccACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTcc                                                                                         >  1:53321/1‑300 (MQ=255)                                                                                                                                                                                               ccACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCNGCAGGCCAGTGTCGATATCGGTACCCNGACCCATGTACCAGGTGCTCTGGCGACCATCTTTGTTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAATGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCAt                                                                                       >  2:432728/1‑300 (MQ=255)                                                                                                                                                                                                    gATCGGTAGTCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCAACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGCCCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTACGGTCAGTATTGGTAAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAGACa                                                                                  >  2:247205/1‑300 (MQ=255)                                                                                                                                                                                                           aaTCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGgaga                                                                           >  2:1149/1‑300 (MQ=255)                                                                                                                                                                                                           aaTCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGgaga                                                                           <  1:215815/300‑1 (MQ=255)                                                                                                                                                                                                                          tttAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGAt                                                            <  1:371606/300‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGCTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCa                                                  <  2:385585/300‑1 (MQ=255)                                                                                                                                                                                                                                         gTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGa                                             >  1:243808/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ggggggggggtgtgaagctggtgtttagaaTAGACGTTCATGGACAGGCTCATCGGCAGGCAAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGAACGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATcc                 >  2:571316/30‑300 (MQ=255)                                                                                                                                                                                                                                                                     gcgcCATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATcc                 >  1:503004/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        ccATAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCa              >  1:336615/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tAGTTCTGCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATaa           <  1:545470/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gCCACTGGTATTTCGCCTAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGa    >  1:386590/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cACTGTTATTTCGCCTAGACATTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGTAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAg  <  2:238032/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                              GACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAG  >  NC_000913/431227‑431801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑