Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	288,641	G→A	A174A (GCC→GCT)	xynR ←	DNA‑binding transcriptional repressor XynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	288,641	0	G	A	100.0%	90.3 / NA	27	A174A (GCC→GCT)	xynR	DNA‑binding transcriptional repressor XynR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/6);  total (21/6)

CTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288375‑288934                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      ctactTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAg                                                                                                                                                                                                                                                                      >  2:306884/1‑300 (MQ=255)                   ggTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGccc                                                                                                                                                                                                                                                    <  1:490844/300‑1 (MQ=255)                            gCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAgg                                                                                                                                                                                                                                           >  1:667288/1‑300 (MQ=255)                                aGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGt                                                                                                                                                                                                                                       >  2:279337/1‑300 (MQ=255)                                                 gcgcgcgAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGccc                                                                                                                                                                                                                      <  1:524872/300‑1 (MQ=255)                                                                         tgctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCg                                                                                                                                                                                              >  1:301034/1‑300 (MQ=255)                                                                                                aGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAg                                                                                                                                                                       >  1:619074/1‑300 (MQ=255)                                                                                                               gtcCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCg                                                                                                                                                        >  1:341136/1‑300 (MQ=255)                                                                                                                                gCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCAGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATggcgg                                                                                                                                       >  2:1706/1‑300 (MQ=255)                                                                                                                                         ggCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGc                                                                                                                              >  1:492818/1‑300 (MQ=255)                                                                                                                                          gCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCAGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGcc                                                                                                                             >  2:634204/1‑300 (MQ=255)                                                                                                                                          gCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCAGATGGCGGTGGCGTGCACCGGCAGGCGGCGGACGATGCGTGAATAGGCGATGGCGGCCAGCTTGcc                                                                                                                             >  1:643035/1‑300 (MQ=255)                                                                                                                                               gggCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGAAGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCaa                                                                                                                        >  1:315989/1‑300 (MQ=255)                                                                                                                                                 gcggcgATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCANGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAAtc                                                                                                                      <  1:441982/300‑1 (MQ=255)                                                                                                                                                                          tCCACACCTGCACCGCCACGCAGCGCACGCCCTGCTCTTTCTCTTCGCTGTCCAGGTCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGNAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGcc                                                                                             <  2:145239/300‑1 (MQ=255)                                                                                                                                                                              cacCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCCGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTNGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTc                                                                                         >  2:578640/1‑300 (MQ=255)                                                                                                                                                                                                gcgcACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGggt                                                                       >  1:358037/1‑300 (MQ=255)                                                                                                                                                                                                    aCGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTc                                                                   <  1:316869/300‑1 (MQ=255)                                                                                                                                                                                                                      tCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGAc                                                 >  1:383509/1‑300 (MQ=255)                                                                                                                                                                                                                      tCGCGGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCGCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGAc                                                 <  2:278118/300‑1 (MQ=255)                                                                                                                                                                                                                           gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  1:491201/1‑300 (MQ=255)                                                                                                                                                                                                                           gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:365208/1‑300 (MQ=255)                                                                                                                                                                                                                           gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGCGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGAGATGGCGGCCAGCTTGCATTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCACAGATGGGTGGTCTGCACGGTCCGCAGGGACAGCTc                                            >  2:119101/1‑300 (MQ=255)                                                                                                                                                                                                                           gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCAAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTCGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCATCGACCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACAGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTAACGCCCGTCCAGAATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTc                                            >  2:332127/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gcgcCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGaa               >  1:539082/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ccttAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAGAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACCCCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCAGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGcc  >  2:397826/3‑300 (MQ=255)                                                                                                                                                                                                                                                                     cATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGcc  >  1:27562/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      CTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288375‑288934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑