Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,324	G→A	G179D (GGC→GAC)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,324	0	G	A	100.0%	89.5 / NA	27	G179D (GGC→GAC)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/17);  total (10/17)

GCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCT  >  NC_000913/287043‑287615                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    tctgctccgcacCGCCGTGAAGGTTTTTGGCTCGCCGTGGAAAAATGGCCGCAACTCTCTCGTCACCGCGCCCTCCATCGAGGGGCCAGGGAACGATCCAGTCCCGATGGGCAACGGGGGGTTTGACCCGTCCCGGTTCCAAGACGAAGAGGGCCGAAAATACGATATATATCGCCCGTGGGGGTTGCGCCACCACAACAACCTGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCCCTCGCTCGGGCGCAAAACGTGGTTTACCGACACGCCGCTCTGCTacac                                                                                                                                                                                                                                                                                   <  2:341733/291‑1 (MQ=255)    gctCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGa                                                                                                                                                                                                                                                                                >  1:286692/1‑300 (MQ=255)                      gATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCTCACCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGTCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGACACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCg                                                                                                                                                                                                                                                              <  2:655510/300‑1 (MQ=255)                                           aaaCGGCCGCAACTTCCTCGTCACCTCGCCCTCCATCGGGGGACCATGGAGCGAGCCAACCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGAAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                         <  2:173058/300‑1 (MQ=255)                                            aaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCTTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTg                                                                                                                                                                                                                                        <  1:545342/300‑1 (MQ=255)                                                                                     gCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtcgt                                                                                                                                                                                               >  1:544722/1‑300 (MQ=255)                                                                                                       cccGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCaaaaa                                                                                                                                                                             <  1:512393/300‑1 (MQ=255)                                                                                                                        ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACg                                                                                                                                                            >  1:656932/1‑300 (MQ=255)                                                                                                                         ttttGACCCTTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACACTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGAGGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGa                                                                                                                                                           <  2:396914/299‑1 (MQ=255)                                                                                                                                 cGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCAcc                                                                                                                                                   <  2:508387/300‑1 (MQ=255)                                                                                                                                      cTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTAACTGATGGCAGCCGAGGGTGGCACCAGCTACGAGCACGCNGTCGTGGTGCTGCGTTCCCAAAATATCGACGGGCCGTACGAGCTGCACCCGGAc                                                                                                                                              >  2:496414/1‑300 (MQ=255)                                                                                                                                         ttCCACGACGACGCTGGCCGCAAATCCTATATCTACCGCCCGTGGGGGCCGCGCCAACACAGCAACCCGCACAACACCCTCGTGTTACAGACGTTTGACCCGCAGACCGGCACACTCTCGCCCGAGCGCAAAATGCTGTTTACCGACACGCCGCTCTGCTACACCGANGACGCGCAACTGTATCGCCACGCGGGAGGGTACTACCTGACGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGAACAGTACGAGACGCACCCGGACgcc                                                                                                                                           >  2:155594/1‑298 (MQ=255)                                                                                                                                         ttCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTNGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTa                                                                                                                                           >  2:491022/1‑300 (MQ=255)                                                                                                                                                              aaaTACTATATCTACCGCCCGGGGGGGCCGCGCCACCACAGCAACCCGGACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACNCTGTTGACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATNGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCAc                                                                                                                      <  2:527858/300‑1 (MQ=255)                                                                                                                                                                       ctcaaCCGCCCGTGGGGGACGTGCAACCAAAGCAACCCGCAAAACACCCTCTTGGGACAGGCGTTGGACCAGCGGACTGACACGCTCTCGCCAGGGCGGAAAACGCTGTGTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAAGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGTGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGgag                                                                                                             <  2:182510/296‑1 (MQ=255)                                                                                                                                                                                      gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTTTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCagaag                                                                                              <  1:616226/300‑1 (MQ=255)                                                                                                                                                                                      gggCCGCGCCACCACAGCAACCCACACAACACCATCGTGTTACAGGCGTTTAACCCGCAGACCGGCACGCTCCCGCCCGAACGCAAACCGCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACTCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCagaag                                                                                              <  2:341925/300‑1 (MQ=255)                                                                                                                                                                                            cgcCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGANGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGc                                                                                        >  2:491799/1‑300 (MQ=255)                                                                                                                                                                                                   acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCt                                                                                 <  1:402775/300‑1 (MQ=255)                                                                                                                                                                                                       cAACCCGCACAACACCATCGTGGTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCgct                                                                             <  1:69451/300‑1 (MQ=255)                                                                                                                                                                                                         aCCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCgctgc                                                                           >  1:355628/1‑300 (MQ=255)                                                                                                                                                                                                                caacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGAc                                                                    >  1:27607/1‑300 (MQ=255)                                                                                                                                                                                                                                     gTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACAt                                               <  2:407409/300‑1 (MQ=255)                                                                                                                                                                                                                                        tGACCCGCAGACCGGCATGCTCTCGCCCGAGCTCAAAACGTTGTTTACCGACACGCCACTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGTGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                            <  2:239353/300‑1 (MQ=255)                                                                                                                                                                                                                                        tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                            <  1:324503/300‑1 (MQ=255)                                                                                                                                                                                                                                        tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGAAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                            <  2:395242/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCgct  >  2:570585/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    GCTGCTCTACACCGACGTGAAGATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCT  >  NC_000913/287043‑287615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑