Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,988	C→T	V15V (GTC→GTT)	tufB →	translation elongation factor Tu 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,988	0	C	T	100.0%	307.7 / NA	94	V15V (GTC→GTT)	tufB	translation elongation factor Tu 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/50);  total (44/50)

TTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCACC  >  NC_000913/4175694‑4176225                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              ttACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTAc                                                                                                                                                                                                                                          <  1:262348/300‑1 (MQ=255)  tACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAANATGTGCTGATATAGCTCAGTTGGTAGACCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACt                                                                                                                                                                                                                                         <  2:480577/300‑1 (MQ=255)     cTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATc                                                                                                                                                                                                                                      <  1:516694/300‑1 (MQ=255)           cTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCAACGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCAc                                                                                                                                                                                                                                >  1:120812/1‑300 (MQ=255)             tCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGTTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGt                                                                                                                                                                                                                              <  2:116656/300‑1 (MQ=255)                  gCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGAcc                                                                                                                                                                                                                         <  1:278805/300‑1 (MQ=255)                   cTGATGATGCGGGTTCGATTCCCGCTGCCCGNTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTNTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCa                                                                                                                                                                                                                        >  2:468816/1‑300 (MQ=255)                     gatgatGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCacg                                                                                                                                                                                                                      >  1:8250/1‑298 (MQ=255)                            ctggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCGTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCNCTCAACAAGTCGGGCATGTTGCCTGGNTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACATACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaac                                                                                                                                                                                                               <  2:451225/298‑1 (MQ=255)                            cGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCANTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTGCTATCGGCCACGTTGACCACGGTAAaac                                                                                                                                                                                                               <  1:453670/300‑1 (MQ=255)                            cGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCNTTCAACAAGTCGGGCATGTTGCCTGGNTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaac                                                                                                                                                                                                               <  2:449958/300‑1 (MQ=255)                            cGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAaac                                                                                                                                                                                                               <  1:583447/300‑1 (MQ=255)                                             gCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCaa                                                                                                                                                                                              <  2:289430/300‑1 (MQ=255)                                                cGCTCCAAGATGTGCTGATATATCTCAGTTGGTAGAGCGCACCCTTGTTAAGGTGGAGGTCGGCAGTTCGAATCTGCCTATCGGCATCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCNACAAGTCGTGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACTTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCa                                                                                                                                                                                           <  2:143832/300‑1 (MQ=37)                                                cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCa                                                                                                                                                                                           <  1:352001/300‑1 (MQ=255)                                                  cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCAcc                                                                                                                                                                                         >  1:218936/1‑299 (MQ=255)                                                    ccAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCAccac                                                                                                                                                                                       <  1:567873/300‑4 (MQ=255)                                                         aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCGGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTAc                                                                                                                                                                                  <  1:33269/300‑1 (MQ=255)                                                         aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTAc                                                                                                                                                                                  <  2:649991/300‑1 (MQ=255)                                                         aTGTACTGATATAGCTCAGTTGGTAGAGCGCACCCTTGTTAAGGTTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGTGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCGGCAATCACCACCGTAc                                                                                                                                                                                  <  2:17321/300‑1 (MQ=37)                                                            tgCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTgg                                                                                                                                                                               >  1:155511/1‑300 (MQ=255)                                                                gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCATGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCAACGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTaa                                                                                                                                                                           >  2:121059/1‑300 (MQ=255)                                                                 atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTACTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATCTCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTATTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCACTCAACACCGTACTGGCTaaa                                                                                                                                                                          >  2:253922/1‑300 (MQ=37)                                                                   ataGCTTATTTGGTAAAGCACACCCTTGGTGAGGGTGAGGTCGGCAGTTTGAATCTGCCTGTCAGCGTCACTTCTTTTCTCTTTCTTTTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGTTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAGAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCTACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAc                                                                                                                                                                        <  2:119973/300‑1 (MQ=21)                                                                   ataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCNTTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAc                                                                                                                                                                        <  1:440089/300‑1 (MQ=255)                                                                      gCTCAGTTGGTAGAGCGCACCCTTGGTAAGGNTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATNTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAAACGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAAcata                                                                                                                                                                     >  2:469976/1‑297 (MQ=255)                                                                          aGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATAACACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTAcggc                                                                                                                                                                 <  1:412384/300‑1 (MQ=255)                                                                               gTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGtgc                                                                                                                                                            <  1:65037/300‑1 (MQ=255)                                                                                 agagCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGtgctg                                                                                                                                                          <  2:631053/300‑1 (MQ=255)                                                                                  gagCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGAACGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGtgccgc                                                                                                                                                         >  2:404704/1‑297 (MQ=255)                                                                                   agCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAGAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTgttgct                                                                                                                                                        >  2:141355/1‑300 (MQ=255)                                                                                   agCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCAATTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCCCGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTgctgct                                                                                                                                                        >  1:520492/1‑300 (MQ=255)                                                                                    gcgcACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTc                                                                                                                                                       <  1:211099/300‑1 (MQ=255)                                                                                         cacTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAAATACTCTGACCGTTGCAATTACCAACGTACTGGCTAAAAACTAAGGCGGTGTTGCTCGCGCa                                                                                                                                                  >  2:129906/3‑300 (MQ=37)                                                                                             tGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTCAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCAACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACAGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCCTTCg                                                                                                                                              >  2:229895/1‑300 (MQ=37)                                                                                             tGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCg                                                                                                                                              >  1:35931/1‑300 (MQ=255)                                                                                             tGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCATGGTTGATGTGGTGATATCAACGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGTGCATTCg                                                                                                                                              >  2:229722/1‑300 (MQ=25)                                                                                                tagggttGAGGTCGGCAGTGCAGATCTGCCTATCAGCACCACCTCTTCTCTCCTCCCTGTTTTTTCCTTCTGTTTAGTGCATTCAACAAGTTGGGCATGTGGCCTGGTTGATTTGGTGATATCACCGATTTATCCGGGGCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAATGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAcc                                                                                                                                           <  2:168313/294‑1 (MQ=18)                                                                                                   gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAACCCTACGGCGGTGCTGCTCGCGCATTCGACCAgg                                                                                                                                        >  2:60853/1‑299 (MQ=255)                                                                                                   gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGa                                                                                                                                        >  1:243936/1‑300 (MQ=255)                                                                                                   gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACAAGa                                                                                                                                        >  2:123170/1‑300 (MQ=255)                                                                                                          gTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATaa                                                                                                                                 <  1:175609/300‑1 (MQ=255)                                                                                                               cAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACgcgc                                                                                                                            <  1:392398/300‑1 (MQ=255)                                                                                                                         cTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCTACCAGATCGATAACGCGCCGGAAGaaaa                                                                                                                  >  1:331074/1‑300 (MQ=255)                                                                                                                           gCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTACTTATGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTCACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCAACACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGCCCAGGTCGATAATGCGCAGGAAGAAAAAg                                                                                                                >  2:127819/1‑300 (MQ=21)                                                                                                                              tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCCACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCCCCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTCCTATCGGCCACGTTGACCACNGTAAAACTACGCTGACCGCTGCAATCAATAACGTACTGGCTAAAAACTACGGCGGTGCTGATCGCAAATTCGACCAggttaatccagcgcagaaagaaacagctc                                                                                                             >  2:589384/1‑272 (MQ=25)                                                                                                                              tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCATGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGATCGATAACGCGCCGGAAGAAGAAGCTc                                                                                                             >  2:212990/1‑300 (MQ=21)                                                                                                                              tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGANCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTc                                                                                                             >  1:462678/1‑300 (MQ=255)                                                                                                                              tATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTATGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAGACCTACGGCGGTGTTGCTCGCGCATTCGAACAGATCGATAACGCGCCGGAAGAAAAAGCTc                                                                                                             >  2:32845/1‑300 (MQ=21)                                                                                                                                        acTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCAc                                                                                                   <  1:295313/300‑1 (MQ=255)                                                                                                                                        acTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCAc                                                                                                   <  2:408717/300‑1 (MQ=255)                                                                                                                                         cttcttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCAcc                                                                                                  >  1:364795/1‑300 (MQ=255)                                                                                                                                          ttcttTTCTCCTCCCTTTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTATATTGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGTAAGAAAAAGCTCGTGGTATCACCa                                                                                                 <  2:166894/300‑1 (MQ=18)                                                                                                                                          ttcttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATTTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCACACGTTAACGTTGGTACGATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACTGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCa                                                                                                 <  2:269122/300‑1 (MQ=21)                                                                                                                                          ttcttTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGGGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCa                                                                                                 <  2:382802/300‑1 (MQ=255)                                                                                                                                               tcccccccccTGCTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAac                                                                                            <  2:76014/293‑1 (MQ=18)                                                                                                                                                 ctcctcCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAacac                                                                                          <  2:6266/300‑1 (MQ=255)                                                                                                                                                  tcctccCTTTTTTTTCCTTCTGTTTATGGCAGTCAACAATTCGGGCATGTTGCCTGGTGTATGTGGTAATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAAGTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTGGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACt                                                                                         <  2:597081/300‑1 (MQ=17)                                                                                                                                                  tcctccCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACt                                                                                         <  1:309668/300‑1 (MQ=255)                                                                                                                                                    ctccCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTtc                                                                                       <  1:16657/300‑1 (MQ=255)                                                                                                                                                         tGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACg                                                                                  <  1:85589/300‑1 (MQ=255)                                                                                                                                                          gTTTTTTCCTTCTGTTTATTGCATTCAACAANTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACANAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCAGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTAACGt                                                                                 >  2:475269/1‑300 (MQ=18)                                                                                                                                                           ttttttCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTTACGtt                                                                                <  2:384183/300‑1 (MQ=255)                                                                                                                                                                 ccTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATAc                                                                          <  1:391111/300‑1 (MQ=255)                                                                                                                                                                    tCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACNTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGac                                                                       <  1:456359/300‑1 (MQ=255)                                                                                                                                                                    tCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGac                                                                       <  1:22121/300‑1 (MQ=255)                                                                                                                                                                     cTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTAACGTTGAATACGaca                                                                      >  1:262619/1‑300 (MQ=255)                                                                                                                                                                       gTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACAcc                                                                    >  1:236463/1‑300 (MQ=255)                                                                                                                                                                              gCATTCAACAAGTCTGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGAccc                                                             >  1:39875/1‑300 (MQ=255)                                                                                                                                                                              gCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGAccc                                                             >  1:39852/1‑300 (MQ=255)                                                                                                                                                                                   caacaaGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCAc                                                        <  1:245005/300‑1 (MQ=255)                                                                                                                                                                                   caacaaGTCGGGCATGTTGCCTGGGTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCAc                                                        <  1:203740/300‑1 (MQ=255)                                                                                                                                                                                             ggCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACg                                              >  1:357753/1‑300 (MQ=255)                                                                                                                                                                                             ggCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGAGCAGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTATAACGTTGAATACGACACCACGAACCGTCACTACGCACACg                                              >  2:248963/1‑300 (MQ=17)                                                                                                                                                                                               catcttGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGAACAGATCGATAAAGCGCAGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCAATACGCATACGTa                                            >  2:114476/5‑300 (MQ=14)                                                                                                                                                                                                  gTTGCCTGGTTGATTTGGTGATATCACCGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAACTTTGAACGTACAAAACCGCACTGTAACGTTGGTACTATCGGCCACGGTGACCACGTTAAAACTACTCTGACCGCTGNAATCACCACCGTACTGGCTAAAACCTNCGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGTAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCGCACGTAGAc                                         <  2:435837/300‑1 (MQ=14)                                                                                                                                                                                                  gTTGCCTGGTTGATGTGGTGATATCACCGATTTGTCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                         <  1:150345/300‑1 (MQ=255)                                                                                                                                                                                                  gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTATAGGGACAATCGATGTCTAAAGAAAAATTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                         <  2:557074/300‑1 (MQ=255)                                                                                                                                                                                                  gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                         <  2:638095/300‑1 (MQ=255)                                                                                                                                                                                                  gTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCGTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGCACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGAc                                         <  2:71187/300‑1 (MQ=255)                                                                                                                                                                                                  gTTGCCTAGGTGATGTGGTAATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACTTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGATCCGTCACTACGCACACGTAGAc                                         <  2:299964/300‑1 (MQ=14)                                                                                                                                                                                                    tGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTg                                       >  1:671046/1‑300 (MQ=255)                                                                                                                                                                                                        tGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTANAACTACTCTGACCGCTGCAATCACCANCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCg                                   >  2:436528/1‑300 (MQ=255)                                                                                                                                                                                                          gTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAANCTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCNTTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCATACGTAGACTGCCCggg                                 >  2:518199/1‑300 (MQ=255)                                                                                                                                                                                                          gTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCggg                                 >  1:607874/1‑300 (MQ=255)                                                                                                                                                                                                            tGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGc                               <  2:8247/300‑1 (MQ=255)                                                                                                                                                                                                                 tggtgATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGcc                          <  1:290631/300‑1 (MQ=255)                                                                                                                                                                                                                  ggtgATATCACCGATTTATCCGTGTCTTAGANGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTNACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGAACCGTCACTACGCACACGTAGACTGCACGGGGCACGCCg                         >  2:473577/1‑300 (MQ=14)                                                                                                                                                                                                                  ggtgATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCACTCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCCCACGTTGAATACGACACCCCGAACCGTCACTACGCATACGTAGACTGCACGGGGCAAGCCg                         >  1:80330/1‑300 (MQ=14)                                                                                                                                                                                                                             cGATTTATCCGTGTCTTAGATGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTaaa              >  1:234178/1‑300 (MQ=255)                                                                                                                                                                                                                               aTTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTaaaaa            >  1:37478/1‑300 (MQ=255)                                                                                                                                                                                                                                       gtgtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCa    >  1:70011/1‑300 (MQ=16)                                                                                                                                                                                                                                         gtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCAcc  >  1:48343/1‑300 (MQ=12)                                                                                                                                                                                                                                         gtCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCTGCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAACTCGTGGTATCACCATCAACACTTCTAACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCAcc  >  2:235471/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              TTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGCGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGTATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAAAACATGATCACC  >  NC_000913/4175694‑4176225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑