Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,277	G→A	R188H (CGT→CAT)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,277	0	G	A	97.8%	154.9 / NA	45	R188H (CGT→CAT)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (16/28);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGC  >  NC_000913/276998‑277571                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       gCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCAt                                                                                                                                                                                                                                                                                    <  1:96294/300‑1 (MQ=255)      gAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAcc                                                                                                                                                                                                                                                                               <  1:576461/300‑1 (MQ=255)       agcgggcgcggaaaaaccggtaaGCGTAGCTGGCAGAGAACCCGCAGGCGGGAATGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGTGGATGGCTCTGAATACCGTTGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTTAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                              <  2:325558/279‑1 (MQ=255)               cggaaaacccGTGAAGCATATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTTGATGCCGGGGCCAATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGACATTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGAc                                                                                                                                                                                                                                                                      <  2:43911/293‑1 (MQ=255)                      cccGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAac                                                                                                                                                                                                                                                               <  1:660520/300‑1 (MQ=255)                         gTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCATGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACAcc                                                                                                                                                                                                                                                            <  1:627679/300‑1 (MQ=255)                           gAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTg                                                                                                                                                                                                                                                          <  1:154862/300‑1 (MQ=255)                             aGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGACGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGAAAGCGAACACCTGAg                                                                                                                                                                                                                                                        >  2:650122/1‑300 (MQ=255)                                           agaACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGNGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCNGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgccgc                                                                                                                                                                                                                                          <  2:608952/300‑1 (MQ=255)                                           agaACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgccgc                                                                                                                                                                                                                                          <  1:666342/300‑1 (MQ=255)                                                     ggCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGt                                                                                                                                                                                                                                >  1:1127/1‑300 (MQ=255)                                                          gAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGgttg                                                                                                                                                                                                                           <  1:349233/300‑1 (MQ=255)                                                                   tggtggCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTgg                                                                                                                                                                                                                  >  2:241953/1‑300 (MQ=255)                                                                             aTCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATcc                                                                                                                                                                                                        <  1:271993/300‑1 (MQ=255)                                                                               ccGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGc                                                                                                                                                                                                      >  2:508006/1‑300 (MQ=255)                                                                                           aCGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCATGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGgcgc                                                                                                                                                                                          <  1:254720/300‑1 (MQ=255)                                                                                              gcgcGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCNCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAg                                                                                                                                                                                       >  1:478011/1‑300 (MQ=255)                                                                                                gcgTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGc                                                                                                                                                                                     <  1:294840/300‑1 (MQ=255)                                                                                                      cTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTNAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGNGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAAc                                                                                                                                                                               <  2:618481/300‑1 (MQ=255)                                                                                                       tGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACt                                                                                                                                                                              <  1:50834/300‑1 (MQ=255)                                                                                                           ggATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTAt                                                                                                                                                                          <  1:326994/300‑1 (MQ=255)                                                                                                                gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                     <  1:587396/300‑1 (MQ=255)                                                                                                                gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                     >  2:20957/1‑300 (MQ=255)                                                                                                                gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTNACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                                     >  1:457210/1‑300 (MQ=255)                                                                                                                        taccttgtcaaTTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCTTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTTAGGCGTTGGCCGAGCTATTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAac                                                                                                                                                             <  2:65209/290‑1 (MQ=255)                                                                                                                        tACCGTCGCGATTATCATTGTAGCGTTGATGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGATGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTNCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGNGCTAGGCATTAACTGTATTGCGCTGGAAAac                                                                                                                                                             <  2:472803/300‑1 (MQ=255)                                                                                                                          ccGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAacac                                                                                                                                                           <  1:271595/300‑1 (MQ=255)                                                                                                                                        aTTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGTGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTgc                                                                                                                                             <  1:72675/300‑1 (MQ=255)                                                                                                                                                     ggCATTTCAGGCGTTTCATCGCCCGCGCTTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGTCGTTGGCCGAGCTGTTGACCGCATATCCGCANGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACgg                                                                                                                                <  2:163753/300‑1 (MQ=255)                                                                                                                                                         tttCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCAACGCTGCGTTGCAGCATTTACACGGTTTa                                                                                                                            >  2:4154/1‑300 (MQ=255)                                                                                                                                                             atgCGTTTCATCGCCCGCGCGTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCGGGCCTGCGAAACTCTGCCGAATTTTTCCGAGATTTAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTTGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACgg                                                                                                                        <  2:123505/298‑1 (MQ=255)                                                                                                                                                             aGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACgg                                                                                                                        <  1:242656/300‑1 (MQ=255)                                                                                                                                                                            cgcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCTAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTTGTTCTCATTTACCCTGCTCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAGCTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCtggtgg                                                                                                         <  2:234070/300‑1 (MQ=255)                                                                                                                                                                                 gTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTAt                                                                                                    >  1:638277/1‑300 (MQ=255)                                                                                                                                                                                          ttGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCg                                                                                           >  1:564736/1‑300 (MQ=255)                                                                                                                                                                                                       gggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACg                                                                              >  1:275777/1‑300 (MQ=255)                                                                                                                                                                                                                                        aTTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCANGTGGTGGCGCTAGGCATTAACTGTATNGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTg                                             <  2:429320/300‑1 (MQ=255)                                                                                                                                                                                                                                             tCCGAGATTGGGGCGTTGGCCGAGCTGTTGACCGCATACCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCGGCGTGACGTGGTTGCGTTGCCGGCGGGTTATCCGCAGGTGGTGGCGCTAGGTATTAACTGTATTGCGCTTGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTACTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACAt                                        <  1:276503/300‑1 (MQ=255)                                                                                                                                                                                                                                                 agaTTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCNTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTgcg                                    >  1:477209/1‑300 (MQ=255)                                                                                                                                                                                                                                                   aTTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTgcgag                                  >  1:283460/1‑298 (MQ=255)                                                                                                                                                                                                                                                              ggCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGa                       <  1:640678/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGAt                      >  1:502743/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ccGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCAGTGAGCAAAACCTGGCATCATCAAGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGt         >  1:603896/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  atCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCAGCAGTGGCAgg   >  1:63337/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGc  <  1:268789/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       GCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGTGGCAGGC  >  NC_000913/276998‑277571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑