Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,572,542	G→A	P322S (CCG→TCG)	eutD ←	phosphate acetyltransferase EutD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,572,542	0	G	A	97.9%	163.6 / NA	48	P322S (CCG→TCG)	eutD	phosphate acetyltransferase EutD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/22);  minor base C (1/0);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTCGCCATCCACCACCAGCTTTGGTGCGCGCT  >  NC_000913/2572249‑2572826                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                             cGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCACCCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGTCACGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGCCACAGc                                                                                                                                                                                                                                                                                        >  1:357424/1‑300 (MQ=255)   ccGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAANCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTg                                                                                                                                                                                                                                                                                      <  1:484024/300‑1 (MQ=255)   ccGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTAAAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGTCCCGGGTTTCGCTCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTg                                                                                                                                                                                                                                                                                      >  2:171378/1‑300 (MQ=255)        aCGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGccacc                                                                                                                                                                                                                                                                                 <  1:282231/300‑1 (MQ=255)        aCGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGCCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGccacc                                                                                                                                                                                                                                                                                 >  2:564926/1‑300 (MQ=255)          gCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAg                                                                                                                                                                                                                                                                               <  1:407570/300‑1 (MQ=255)             gtgCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCAGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAgcgc                                                                                                                                                                                                                                                                            >  1:598178/1‑300 (MQ=255)                  gCTGCGGCACCTGCATCGTTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACTGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCt                                                                                                                                                                                                                                                                       <  1:108183/300‑1 (MQ=255)                                          gCTTTACGGGCTGCAACATCACCACGAACCAGGGCAGGATACAGGCCGCCGCCAATTTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCATGCCCCTGTTTTCGATCATTCCGAATGCTTCAATTGTGCTTTCCTCGTTATCAGCGTCCAGAACGGGACCATTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGAGAATTTCCTGCACGCTACAAc                                                                                                                                                                                                                                               <  2:167630/300‑1 (MQ=255)                                          gCTTTACAGGCTGCAACATCACCACGAACCATTGCAGTACACAGGCCACCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCATGCCCCGGGTTTCGATCATTCCTAAGTCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGTCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAAc                                                                                                                                                                                                                                               <  2:123020/300‑1 (MQ=255)                                          gCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTAAAAc                                                                                                                                                                                                                                               >  2:360229/1‑300 (MQ=255)                                                  ggCTGCAACATCACCACGAACCGTGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGTCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag                                                                                                                                                                                                                                       <  2:204831/300‑1 (MQ=255)                                                  ggCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACgagag                                                                                                                                                                                                                                       >  1:335079/1‑300 (MQ=255)                                                      gcagcATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCACGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCATGCCCCTGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGGCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGAt                                                                                                                                                                                                                                   <  2:388473/296‑1 (MQ=255)                                                        aaCATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCg                                                                                                                                                                                                                                 <  1:290227/300‑1 (MQ=255)                                                                cacGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCgg                                                                                                                                                                                                                         <  1:512572/300‑1 (MQ=255)                                                                   gAACAATGGCAGTAAAAAGGCCGCCGCCAATCTGCTTAACACCAAACAGCTTGACGCGCGCAGCGTTCACAAGCGCGTCAGAAGCCTCAATCGGGGCAACCAGGCCCCGGGTGTCGGTCATTCCTAATGCTTCCATTGTGCTTTCCTCGTGATCAGGGGCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACGGCTTTCGCGGTGCACGTCGGGCTGACGCGACACAGAGGAAACAAGCGGAGGCTCGATAATTTCCTACACGCTACAACCACGAGAGAGATCGTGCATCGgcgc                                                                                                                                                                                                                      <  2:25929/300‑1 (MQ=255)                                                                    aaCCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGcgcg                                                                                                                                                                                                                     >  1:671208/1‑300 (MQ=255)                                                                      ccATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCAGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAAACACGAGAGAGATCGTGCATCGGCGCGGc                                                                                                                                                                                                                   >  2:418914/1‑300 (MQ=255)                                                                           gCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTc                                                                                                                                                                                                              >  1:489358/1‑300 (MQ=255)                                                                              gTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCtt                                                                                                                                                                                                           >  1:190136/1‑300 (MQ=255)                                                                                acacAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCANCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATNTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGt                                                                                                                                                                                                         >  2:613934/1‑300 (MQ=255)                                                                                acacAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCANCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCAGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATNTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGt                                                                                                                                                                                                         >  2:615123/1‑300 (MQ=255)                                                                                acacAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGt                                                                                                                                                                                                         >  1:369016/1‑300 (MQ=255)                                                                                     ggCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATGCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAg                                                                                                                                                                                                    <  2:116235/300‑1 (MQ=255)                                                                                     ggCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCGTTCACCATCGCGGCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGGCGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGATAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAg                                                                                                                                                                                                    <  1:280097/300‑1 (MQ=255)                                                                                          ccgccAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGcc                                                                                                                                                                                               >  1:640984/1‑300 (MQ=255)                                                                                                    gCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGCCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTATGTCTGACGCGACACAGCTGCAACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACg                                                                                                                                                                                     >  2:627586/1‑300 (MQ=255)                                                                                                        aaCACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGatat                                                                                                                                                                                 <  1:263164/300‑1 (MQ=255)                                                                                                          cacCAGCCGGCTTGACGCGCGCAGCCTTAACCATCGCGTCAGAAGCTTCAATCAGTACAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACTGGACCGTTCATTCAACCAGTGTTTGTAAGCTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCGGCACGCTACAACCACGAGAGAAATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATcc                                                                                                                                                                               <  2:35791/300‑1 (MQ=255)                                                                                                                                 gCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCAGGGTTTCGATCATTACTAATGCTTCCATTGTGCTTTCCTCTTTATCGGGGTCCAGAACGGGACCGTTCATTCCACCAGTGTTTATAAACTGCTTTCGCGGTTTACTTCTGTATGACGCGACACAGCTGCCGCCAGCGCCAGCTCGATAATTTCCTGCACGTTACAACCACGAGAGAGACCGCGCATCGGCGCGGAAAGTCCTTGCACCAGTGGCACGAAGGCACGATATCCGACGAGTCGTTGTGTGATTTTGt                                                                                                                                                        >  2:81077/1‑300 (MQ=255)                                                                                                                                        ccATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTACATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAat                                                                                                                                                 >  1:267864/1‑300 (MQ=255)                                                                                                                                                                gTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGaaa                                                                                                                         >  1:633230/1‑300 (MQ=255)                                                                                                                                                                   cAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCAGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAAc                                                                                                                      >  1:365926/1‑300 (MQ=255)                                                                                                                                                                    aaCCATGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACTGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAAcc                                                                                                                     <  2:169539/300‑1 (MQ=255)                                                                                                                                                                                ggTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGcc                                                                                                         <  1:289124/300‑1 (MQ=255)                                                                                                                                                                                ggTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGcc                                                                                                         <  2:656317/300‑1 (MQ=255)                                                                                                                                                                                           ttCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTCAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGAAGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAg                                                                                              >  1:669186/1‑300 (MQ=255)                                                                                                                                                                                           ttCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACNCAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAg                                                                                              <  1:443469/300‑1 (MQ=255)                                                                                                                                                                                           ttCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAg                                                                                              >  1:18723/1‑300 (MQ=255)                                                                                                                                                                                                                   ccTCTTTATCAGGGTCCGGAACTGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTgcgc                                                                      <  2:655125/300‑1 (MQ=255)                                                                                                                                                                                                                              gggTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCg                                                           >  1:6053/1‑300 (MQ=255)                                                                                                                                                                                                                                gTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGc                                                         >  2:273394/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGNCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGNAggcgg                                               >  2:540650/1‑300 (MQ=255)                                                                                                                                                                                                                                                          cAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTc                               <  1:416308/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      aCTGCTTTCGCGGTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGNGTCGTTGTGCGATTTTGTAACCAATANTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTCGCCATccaccac                   <  2:436435/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTTCACTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTCGCCATCCACCACCAGCTTTGGTgc       >  1:81641/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       cTTCTGTCTGACGCGACACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTCGCCATCCACCACCAGCTTTGGTGCGCGCt  >  1:124274/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                             CGCCGATACGCTGTGCCGCTGCGGCACCTGCATCGGTCGCGGCTTTACAGGCTGCAACATCACCACGAACCATGGCAGTACACAGGCCGCCGCCAATCTGCTTAACACCAACCAGCTTGACGCGCGCAGCTTTCACCATCGCGTCAGAAGCCTCAATCAGTGCAACCAGGCCCCGGGTTTCGATCATTCCTAATGCTTCCATTGTGCTTTCCTCTTTATCAGGGTCCAGAACGGGACCGTTCATTCAACCAGTGTTTGTAAACTGCTTTCGCGGTTCACTTCTGTCTGACGCGGCACAGCTGCCACCAGCGCCAGCTCGATAATTTCCTGCACGCTACAACCACGAGAGAGATCGTGCATCGGCGCGGCAAGTCCTTGTATCAGTGGCCCGACGGCACGATATCCGCCGAGTCGTTGTGCGATTTTGTAACCAATATTTCCGGCTTCCAGCGACGGAAAAACCATCACATTGGCCTTGCCCTGTAGCGGGCTGGCAGGCGCTTTTTGCGCCGCCACTTCCGGCACGAAGGCGGCGTCAAACTGTAACTCGCCATCCACCACCAGCTTTGGTGCGCGCT  >  NC_000913/2572249‑2572826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑