Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	268,709	G→A	R84R (CGC→CGT)	ykfA ←	putative GTP‑binding protein YkfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	268,709	0	G	A	100.0%	140.5 / NA	41	R84R (CGC→CGT)	ykfA	putative GTP‑binding protein YkfA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/15);  total (26/15)

CCGTGGACAACTGTCCCCCACCGCTGGTAGGTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAG  >  NC_000913/268453‑269003                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       ccGTGGACAACTGTCCCCCACCGCTGGTAGGTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTAc                                                                                                                                                                                                                                                             <  1:88415/300‑1 (MQ=255)   gTGGACAACTGTCCCCCACCGCTGGTAGGTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAg                                                                                                                                                                                                                                                           >  1:25720/1‑300 (MQ=255)                ccccACCGCTGGTAGGTTCTGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGAGAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCTAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGc                                                                                                                                                                                                                                              <  2:167317/300‑1 (MQ=255)                  ccACCGCTGGTAGGTTCGGCCTTATCCTACTTGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGTAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTg                                                                                                                                                                                                                                            <  2:208671/300‑1 (MQ=255)                       gCTGGTAGGTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCgg                                                                                                                                                                                                                                       >  1:246491/1‑300 (MQ=255)                              ggTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatata                                                                                                                                                                                                                                >  1:504308/1‑300 (MQ=255)                               gTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatatat                                                                                                                                                                                                                               <  1:596945/300‑1 (MQ=255)                               gTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatatat                                                                                                                                                                                                                               >  2:638280/1‑300 (MQ=255)                                    accccacccaaccaactaaTAACAAACAACACCCTACGCCAGTATACCTCTCCAATCACCTGACGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTACAGGCGAAAGCGCAGCGGCTCACACATACAGGCCGCCACATCACTGACCGGTGATATATCTCCg                                                                                                                                                                                                                          >  2:240219/19‑300 (MQ=25)                                                      aTAACANACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAANGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGcac                                                                                                                                                                                                        <  2:547843/300‑1 (MQ=255)                                                          caagtaGCACCTTATGCCGGTATACCTCTCCAAGCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACATGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGcacatac                                                                                                                                                                                                    <  2:287088/295‑4 (MQ=255)                                                                          ccGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCCGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGAATGCTCTTCCCAACAcc                                                                                                                                                                                    >  1:553390/1‑300 (MQ=255)                                                                           cGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTACCAACACCg                                                                                                                                                                                   >  2:25039/1‑300 (MQ=255)                                                                           cGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTACAAACACCg                                                                                                                                                                                   >  2:201849/1‑300 (MQ=255)                                                                                                                               cccGGTCATCGGCCTTAATCAGCCACAGTACCAGTTCGAGGCGAGTAAGCTGTTCGCGGTACAGCGCAGCATATTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAAttt                                                                                                                               <  2:345128/300‑1 (MQ=255)                                                                                                                                          gCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATANATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGc                                                                                                                    >  1:484529/1‑300 (MQ=255)                                                                                                                                                     ccACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATAc                                                                                                         >  1:137575/1‑300 (MQ=255)                                                                                                                                                       acaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATAccc                                                                                                       <  2:311038/300‑1 (MQ=255)                                                                                                                                                       acaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATAccc                                                                                                       <  1:410630/300‑1 (MQ=255)                                                                                                                                                          gTACCAGGTCGAGGCGCGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGCTACCCAtt                                                                                                    >  2:363336/1‑300 (MQ=255)                                                                                                                                                          gTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCTGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCAtt                                                                                                    >  1:660437/1‑300 (MQ=255)                                                                                                                                                          gTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACGCCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCAtt                                                                                                    >  2:565499/1‑300 (MQ=255)                                                                                                                                                          gTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCAtt                                                                                                    >  1:567349/1‑300 (MQ=255)                                                                                                                                                            aCCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGc                                                                                                  <  1:580559/300‑1 (MQ=255)                                                                                                                                                                                  gTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCt                                                                            >  1:630585/1‑300 (MQ=255)                                                                                                                                                                                      gcgGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGaaa                                                                        <  1:506813/300‑1 (MQ=255)                                                                                                                                                                                         gTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCNCATCGCTGACCGGTGATATATCTCCGNCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGa                                                                     >  2:435953/1‑300 (MQ=255)                                                                                                                                                                                              gcgcAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCa                                                                >  2:231036/1‑300 (MQ=255)                                                                                                                                                                                              gcgcAGCATACTCGGTATCGCGAGCGCAACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCa                                                                >  2:358695/1‑300 (MQ=255)                                                                                                                                                                                                               tCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATGTAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATtc                                               <  2:259344/300‑1 (MQ=255)                                                                                                                                                                                                               tCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGTTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATtc                                               <  1:628416/300‑1 (MQ=255)                                                                                                                                                                                                                            tttCGCCCACGCCGGGCAGATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTActcctc                                  <  1:394181/300‑1 (MQ=255)                                                                                                                                                                                                                                              gATCCATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTACGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAAc                >  1:320966/1‑300 (MQ=255)                                                                                                                                                                                                                                                  cATCAGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTa            >  1:303358/1‑300 (MQ=255)                                                                                                                                                                                                                                                      aGCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAAt        <  1:544088/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATc       >  1:318831/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTACGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTATGAAAAATAATAAAAAACGGTAGAATc       >  2:515414/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gCGTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGCCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACAGTAGAATc       >  2:515546/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTTATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGt     >  1:555789/1‑300 (MQ=255)                                                                                                                                                                                                                                                          ttATATAACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTg    >  2:646007/1‑300 (MQ=255)                                                                                                                                                                                                                                                            atataACGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTACGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTgag  >  1:303837/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                       CCGTGGACAACTGTCCCCCACCGCTGGTAGGTTCGGCCTTATCCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACACCGGTTTTACCCATAATGCCGATCACTGGCTCGTAGTTAGTTAACTGGTTAATTTGCTGCAGAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAG  >  NC_000913/268453‑269003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑