Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	267,149	G→A	intergenic (‑596/+35)	yafY ← / ← yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	267,149	0	G	A	100.0%	180.1 / NA	51	intergenic (‑596/+35)	yafY/yafZ	inner membrane lipoprotein YafY/DUF932 domain‑containing protein YafZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/29);  total (22/29)

CACCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAG  >  NC_000913/266853‑267444                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        cacCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGatgt                                                                                                                                                                                                                                                                                                      >  1:203157/1‑300 (MQ=255) cacCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGAACGTCAGCACGGTGCGATGC‑GGACAGGTGatgt                                                                                                                                                                                                                                                                                                      >  2:64754/1‑300 (MQ=255)            gCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATTAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAAc                                                                                                                                                                                                                                                                                           <  2:202172/300‑1 (MQ=255)                      tACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCaa                                                                                                                                                                                                                                                                                 >  1:224029/1‑300 (MQ=255)                      tACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCGGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCaa                                                                                                                                                                                                                                                                                 <  2:222754/300‑1 (MQ=255)                         gCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGGGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACAt                                                                                                                                                                                                                                                                              <  1:42500/300‑1 (MQ=255)                            cccTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTaa                                                                                                                                                                                                                                                                           >  1:621059/3‑300 (MQ=255)                                cAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGTTCATGGCGGCGTTCTGCCTGTGTCATTAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACgg                                                                                                                                                                                                                                                                       <  2:641312/300‑1 (MQ=255)                                            tGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTg                                                                                                                                                                                                                                                           <  1:60910/300‑1 (MQ=255)                                             gCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGc                                                                                                                                                                                                                                                          >  2:630263/1‑300 (MQ=255)                                                        gCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTTTCATTAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGc                                                                                                                                                                                                                                               <  1:174014/300‑1 (MQ=255)                                                               aTCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGcc                                                                                                                                                                                                                                        >  1:605808/1‑300 (MQ=255)                                                                        agcctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCcac                                                                                                                                                                                                                               <  1:124707/300‑1 (MQ=255)                                                                          cctgacagccAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAg                                                                                                                                                                                                                             <  1:621517/300‑1 (MQ=255)                                                                                             tGGCGGCGTTCTGCCTGTGTCATTAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACg                                                                                                                                                                                                          <  1:238663/300‑1 (MQ=255)                                                                                             tGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGCTGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATATCCGATATTAACAAAGCAGCAACATTCCACGGTAACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCAGGTTAAGTTTCACg                                                                                                                                                                                                          >  2:286044/1‑300 (MQ=255)                                                                                                    gTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGt                                                                                                                                                                                                   >  1:554663/1‑300 (MQ=255)                                                                                                    gTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCAGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCCTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGt                                                                                                                                                                                                   >  2:630211/1‑300 (MQ=255)                                                                                                                              ccGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTNCCCTCGGTGAGTGAGGACCGTCAGCANGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCGCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTAtgt                                                                                                                                                                         >  2:435570/1‑300 (MQ=255)                                                                                                                                            tgactgaAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑NGACAGGTGATGTCCGATATTAACAAANCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCttt                                                                                                                                                           <  2:451623/300‑1 (MQ=255)                                                                                                                                                  aaaaTGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCAAGAGGCAGCGGGGGCTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTACACGGTCAATGAAGTTGCGTGAGCAGGGCTTACGCCCTCAACCACAGTGCCCGGTTAAGTTTCAAGTCCCCGTTGATACCGCGAACGGCACGGGTATGTGCCCGTCCTCCTTTGGCAtt                                                                                                                                                     >  2:393699/1‑300 (MQ=255)                                                                                                                                                                aCTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAgcc                                                                                                                                       <  1:494713/300‑1 (MQ=255)                                                                                                                                                                     aaaGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAgcccgccc                                                                                                                                  <  1:83332/300‑1 (MQ=255)                                                                                                                                                                                    cAGGGTCTGGACACCACAGAAACTATTTTTCAGTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTAATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTAGGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTcc                                                                                                                   <  2:123848/300‑1 (MQ=255)                                                                                                                                                                                      gggTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACNGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTg                                                                                                                 >  1:448102/1‑300 (MQ=255)                                                                                                                                                                                      gggTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGNCCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTNCTCCTg                                                                                                                 >  2:550693/1‑300 (MQ=255)                                                                                                                                                                                      gggTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTg                                                                                                                 >  1:69802/1‑300 (MQ=255)                                                                                                                                                                                      gggTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTg                                                                                                                 >  2:559203/1‑300 (MQ=255)                                                                                                                                                                                      gggTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCAACCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCATCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTg                                                                                                                 >  2:559195/1‑300 (MQ=255)                                                                                                                                                                                           tGGACACCACATAAACTATTTTTCATTTCTGCAAAAGCCATAGGCAGCGGGGTTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATAc                                                                                                            <  2:282907/300‑1 (MQ=255)                                                                                                                                                                                           tGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATAc                                                                                                            <  1:588455/300‑1 (MQ=255)                                                                                                                                                                                            ggACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACg                                                                                                           >  1:72038/1‑300 (MQ=255)                                                                                                                                                                                             gACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGc                                                                                                          <  1:631469/300‑1 (MQ=255)                                                                                                                                                                                                   acataaaCTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGGTGC‑GGACAGTTGATGTCCGATATTAACAGAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTAc                                                                                                    <  2:24383/296‑1 (MQ=255)                                                                                                                                                                                                    caGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTAACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACg                                                                                                   <  1:204795/300‑1 (MQ=255)                                                                                                                                                                                                               tttCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACTGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAgg                                                                                        <  2:124947/300‑1 (MQ=255)                                                                                                                                                                                                               tttCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAgg                                                                                        <  2:315074/300‑1 (MQ=255)                                                                                                                                                                                                               tttCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAgg                                                                                        <  1:366982/300‑1 (MQ=255)                                                                                                                                                                                                               cttCATGTCTGCAAAAGACAGAGGCAGCTGGTGTGACAGGCCTTCCCTCGGTGAGTGAAGACCGTCAGCACTGTGCGGGGCAGGA‑AGGTGAGGGCAGATATTAAAAAAGCAACGACATTAAACGGTCACTGCAGTTGCGTGAGCAGGACTTCCGCCATCACCCGCATTGCCCAGTTAAGTGTCACGTCCCCTTCGATACCGTGAACGGCACGGGTATGTGACCTTCCTCCTTTGGCATTACGGCCACTGGGCCCGCCCTGAGTCAGGTTCTCCTGAATACGCTGGTGCGTGGTCCACAgg                                                                                        <  2:9214/299‑1 (MQ=255)                                                                                                                                                                                                                        tGCAAAAGCCAGATGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGctc                                                                               <  1:356448/300‑1 (MQ=255)                                                                                                                                                                                                                        tGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGctc                                                                               <  1:356598/300‑1 (MQ=255)                                                                                                                                                                                                                             aaGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATc                                                                          <  1:633298/300‑1 (MQ=255)                                                                                                                                                                                                                                        aGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCNTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACNGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCATGAGTACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAgcggc                                                               >  2:526218/1‑300 (MQ=255)                                                                                                                                                                                                                                             gggTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAggg                                                          >  1:105822/1‑300 (MQ=255)                                                                                                                                                                                                                                               gTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGga                                                        >  1:492324/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGa                                                   <  1:378613/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gagGACCGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCgg                                >  1:615899/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             cGTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCAACAGTGCCCGGTTAAGTTTCACGNCCCCGTCGATACCGCGAACGGCACGGNTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCATTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCtggtg                          >  2:452945/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gTCAGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCtggtgg                         <  1:155293/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aGCACGGTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCNGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCt                      <  2:581589/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ggTGCGATGC‑GGACAGGGGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTGTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCAcc                 <  2:118210/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gTGCGATGC‑GGACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTCCGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCACCa                >  1:612937/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ggACAGGTGATGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCACGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCACCAAAGCGGTAt       >  2:339711/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      ggtgatGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAg  >  2:402635/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        CACCAAACTCAGCGGCAAGCTTACGCACACTCAGCGTTTCCCCTGCCACCAGACGGCTGATTATCAGTGACAGCCTGACAGCCAGCCGGTCATGGCGGCGTTCTGCCTGTGTCATGAGACGTTCTCCGTGAAAGTTAACTGACTGAAAATGATGTGATTACTTTAAAGAGGGGGTCGGACAGGGTATGGACACCACAGAAACTATTTTTCATTTCTGCAAAAGCCAGAGGCAGCGGGGGTTACAGGCCTTCCCTCGGTGAGTGAGGACCGTCAGCACGGTGCGATGC‑GGACAGGTGGTGTCCGATATTAACAAAGCAGCAACATTAAACGGTCACTGCAGTTGCGTGAGCAGGGCTTCCGCCATCACCCACAGTGCCCGGTTAAGTTTCACGTCCCCGTCGATACCGCGAACGGCACGGGTATGTGACCGTCCTCCTTTGGCATTACGGCCACTGAGCCCGCCCTTAATCAGGTTCTCCTGAATACGCTGGTACGTGGTCCACAGGTCATTGCTCTCATCCTGCCAGCGGCGAGGGGAGAGGATCTGCGATTCAGTCACCGGCTGGTGGTCTTCACCAAAGCGGTATGTGAG  >  NC_000913/266853‑267444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑