Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3822646 =2 (0.020)110 (1.290) 64/598 0.1 94.8% coding (247/2109 nt) spoT bifunctional (p)ppGpp synthase/hydrolase SpoT
?NC_000913 = 3822651 10 (0.120)coding (252/2109 nt) spoT bifunctional (p)ppGpp synthase/hydrolase SpoT

Only 100 of 230 total aligned reads displayed.

TTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAG  >  NC_000913/3822362‑3822949
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
ttgctgaaggtcgtcgttaatcacaaagcggttcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATgg                                                                                                                                                                                                                                                                                                        >  1:320988‑M1/291‑300 (MQ=255)
 tgctgaaggtcgtcgttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggnaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGa                                                                                                                                                                                                                                                                                                       >  1:480747‑M1/290‑300 (MQ=255)
               gttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcaagcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGgtc                                                                                                                                                                                                                                                                                         <  2:557492‑M1/25‑2 (MQ=255)
                                   ccctcgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacntaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGANCTGGTa                                                                                                                                                                                                                                                                     <  2:553238‑M1/45‑1 (MQ=255)
                                   cccTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGCTACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgacgagctggta                                                                                                                                                                                                                                                               <  2:215478‑M2/300‑46 (MQ=255)
                                   cccTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AA‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggta                                                                                                                                                                                                                                                               <  2:563865‑M2/300‑46 (MQ=255)
                                    ccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcttctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTag                                                                                                                                                                                                                                                                    >  2:371722‑M1/255‑300 (MQ=255)
                                    ccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccaccnaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGNTGGTag                                                                                                                                                                                                                                                                    <  2:543546‑M1/46‑1 (MQ=255)
                                    ccTTGTATCTGTTTGAAAGCCTGAATCAACCGATTCAAACCTACCTGCCGGAATACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCTAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtag                                                                                                                                                                                                                                                              >  2:43256‑M2/1‑254 (MQ=255)
                                        gtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggcctagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAggg                                                                                                                                                                                                                                                                >  1:238102‑M1/251‑300 (MQ=255)
                                        gTATCTGTTTGAGAGCCCGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCGCACTAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagaggg                                                                                                                                                                                                                                                          >  2:16670‑M2/1‑250 (MQ=255)
                                        gTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCTAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagccggtagaggg                                                                                                                                                                                                                                                          >  2:630967‑M2/1‑250 (MQ=255)
                                          aTCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagaggggg                                                                                                                                                                                                                                                        >  1:25138‑M2/1‑248 (MQ=255)
                                            ctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGGGGgtg                                                                                                                                                                                                                                                            <  1:203437‑M1/54‑1 (MQ=255)
                                              gtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcntctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgacacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctacaaggatCNGGATATGGAACAGCTTTTTGGTAACAGCGTCGACGAGCTGGTAGGGGGGGTGTc                                                                                                                                                                                                                                                          <  2:617600‑M1/56‑1 (MQ=255)
                                              gtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTc                                                                                                                                                                                                                                                          <  2:656275‑M1/56‑1 (MQ=255)
                                                   aaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAAc                                                                                                                                                                                                                                                     >  1:663988‑M1/240‑300 (MQ=255)
                                                                  tGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACTAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagt                                                                                                                                                                                                                                >  2:33125‑M2/1‑224 (MQ=255)
                                                                    attcaaacctacctgccggaagaccaaatcaagcgcctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTc                                                                                                                                                                                                                                    >  1:24434‑M1/223‑300 (MQ=255)
                                                                               cctgccngaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgangctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATaagaa                                                                                                                                                                                                                         >  2:537828‑M1/212‑300 (MQ=255)
                                                                                 tGCCGGAAGACCAAATCAAATGTCTGCTACAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGGTCAAGCGGTGAACTCTATATCACGCACTCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATTAAACGCTGATGGCGGCGCTGCTGCATGACTTGATTGAAGATACTCCCGCCACTT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaag                                                                                                                                                                                                                 >  2:138385‑M2/1‑209 (MQ=255)
                                                                                  gCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaaga                                                                                                                                                                                                                <  2:505084‑M2/300‑93 (MQ=255)
                                                                                              aatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGAGAAGGCCGa                                                                                                                                                                                                          <  2:352188‑M1/104‑1 (MQ=255)
                                                                                                                ggCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACNCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgatanactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgat                                                                                                                                                                                  <  2:624601‑M2/300‑123 (MQ=255)
                                                                                                                ggCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgat                                                                                                                                                                                  <  1:145680‑M2/300‑123 (MQ=255)
                                                                                                                           ttGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCAAGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTTATGGCGGCGCTGCTGCATGGCGTGAGTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatagaacagctttttggtaaaagcgtcgacgagctggtagagggggtgtcggcacttgctcaactaaagttccgcgataagaaaaaggcgccggccgaaaactttcgcaagatgattatggcgatgg                                                                                                                                                                       <  2:229189‑M2/300‑134 (MQ=255)
                                                                                                                             gcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaacaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTCTCGCCAGATGATTCTGGCGATGGTg                                                                                                                                                                           <  1:136470‑M1/135‑1 (MQ=255)
                                                                                                                                 ttgatgctcacgaggggcaaacacggtcaagcggtgaaccctatatcacgcacccggtagcgggtgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAgg                                                                                                                                                                       >  1:362587‑M1/162‑300 (MQ=255)
                                                                                                                                   gatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGat                                                                                                                                                                     >  1:602138‑M1/160‑300 (MQ=255)
                                                                                                                                    atgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaacaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGGTGATTATGGCGATGGTGCAGGata                                                                                                                                                                    <  2:105742‑M1/142‑1 (MQ=255)
                                                                                                                                    aTGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggata                                                                                                                                                              <  1:526499‑M2/300‑143 (MQ=255)
                                                                                                                                      gctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGGTATc                                                                                                                                                                  <  2:70788‑M1/144‑1 (MQ=255)
                                                                                                                                        tCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGTTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCNGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttngtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccg                                                                                                                                                          >  2:521735‑M2/1‑154 (MQ=255)
                                                                                                                                           cGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgt                                                                                                                                                       <  1:255160‑M2/300‑150 (MQ=255)
                                                                                                                                               gggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGNAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATc                                                                                                                                                         <  1:479751‑M1/153‑1 (MQ=255)
                                                                                                                                                ggcaaataccgtcaagcggtgaaCCCTATACCACGCACCCGGTAACGGTGGTCTACATTCTGGCCTAGATGAAACTCGACTATTAAACGTTGATGGCGGTGCTGCTGCATGACTGGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacggctttgtggtaaaagcgtcgccgagctggtagaggggatgtcgaaacttgagaaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatggtgatggcgatggtgcaggatatccgcgtcatcc                                                                                                                                                  >  2:63298‑M2/12‑146 (MQ=255)
                                                                                                                                                ggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGGCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATcc                                                                                                                                                        >  1:556068‑M1/147‑300 (MQ=255)
                                                                                                                                                         gTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaac                                                                                                                                         >  1:33361‑M2/1‑137 (MQ=255)
                                                                                                                                                                cggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGa                                                                                                                                        <  2:353834‑M1/170‑1 (MQ=255)
                                                                                                                                                                      accctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAc                                                                                                                                  <  1:142106‑M1/176‑1 (MQ=255)
                                                                                                                                                                      accctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGANTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAACCTTGCCGNCCGTAc                                                                                                                                  <  2:541104‑M1/176‑1 (MQ=255)
                                                                                                                                                                      aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataaggaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgcccgtac                                                                                                                            <  2:567929‑M2/300‑177 (MQ=255)
                                                                                                                                                                      aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtac                                                                                                                            <  2:414665‑M2/300‑177 (MQ=255)
                                                                                                                                                                      aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgantatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgnccgtac                                                                                                                            <  2:545970‑M2/300‑177 (MQ=255)
                                                                                                                                                                      aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaacncaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtac                                                                                                                            <  2:589897‑M2/300‑177 (MQ=255)
                                                                                                                                                                      aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgacgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtac                                                                                                                            <  1:642987‑M2/300‑177 (MQ=255)
                                                                                                                                                                       ccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAcc                                                                                                                                 <  1:548398‑M1/177‑1 (MQ=255)
                                                                                                                                                                        ccTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtaccc                                                                                                                          >  2:420229‑M2/1‑122 (MQ=255)
                                                                                                                                                                         cTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtaccca                                                                                                                         <  1:348920‑M2/300‑180 (MQ=255)
                                                                                                                                                                         cTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgaaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtaccca                                                                                                                         <  2:130567‑M2/300‑180 (MQ=255)
                                                                                                                                                                             atCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaac                                                                                                                     >  1:299974‑M2/1‑117 (MQ=255)
                                                                                                                                                                             atCACGCACCCGGTAGCGGGTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑NC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatttcgatggtgcaggatatccgcgtcatcctcatcaaacctgccgaccgtacccacaac                                                                                                                     >  1:481096‑M2/1‑117 (MQ=255)
                                                                                                                                                                             agcacgcacccgggagcggttgcctgcattctggccgagatgaagctcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATACGGAACAGNTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGTCGATGGTGCAGGATATCCGCTTCATCCTCATCAAACTTGCCGACCGTACCCacaac                                                                                                                           >  2:149962‑M1/118‑300 (MQ=255)
                                                                                                                                                                             agcacacACCCGGTAGTGGTTGGCTGTATTCTGGACGAGAGGAAACTTGACTATGAAACGCCGATGGTGGCGCTGCTGCACGGCGTGGTTGAAGATACTCCCACCACCTGACAGGATCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAGACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCacaac                                                                                                                           <  1:365878/294‑1 (MQ=255)
                                                                                                                                                                                  gcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATgcg                                                                                                                      >  1:560749‑M1/113‑300 (MQ=255)
                                                                                                                                                                                     cccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCAc                                                                                                                   <  1:608699‑M1/191‑1 (MQ=255)
                                                                                                                                                                                       cGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgc                                                                                                           <  2:113782‑M2/300‑194 (MQ=255)
                                                                                                                                                                                       cGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctntttggtaaaagcgtcgccgagctggtngagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgc                                                                                                           <  2:446232‑M2/300‑194 (MQ=255)
                                                                                                                                                                                            gcggttgcctgcattctggccgagatgaaactcgactatgaaacgctggtggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGc                                                                                                            <  1:114042‑M1/198‑1 (MQ=255)
                                                                                                                                                                                                 tgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACt                                                                                                       >  1:312022‑M1/98‑300 (MQ=255)
                                                                                                                                                                                                 tgcctgcattctggccgagatgaaactcgacnatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAANGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACt                                                                                                       <  2:471001‑M1/203‑1 (MQ=255)
                                                                                                                                                                                                 tGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcact                                                                                                 >  2:303042‑M2/1‑97 (MQ=255)
                                                                                                                                                                                                                   gatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTcg                                                                                     <  1:92843‑M1/221‑1 (MQ=255)
                                                                                                                                                                                                                            cgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGc                                                                            <  1:325441‑M1/230‑1 (MQ=255)
                                                                                                                                                                                                                              actatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGNCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGACCGGACAAATGTCGCCGCATcgcac                                                                          <  2:156883‑M1/232‑3 (MQ=255)
                                                                                                                                                                                                                               gtatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaagatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGTAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAAAATGCGCACGATGGGCTCACTTAGCACGAACAAACGTCGCAGcatcgactg                                                                         <  2:654540‑M1/233‑5 (MQ=255)
                                                                                                                                                                                                                                                     ctgctgCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagc                                             <  2:224773‑M2/300‑256 (MQ=255)
                                                                                                                                                                                                                                                       gctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGCAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGccc                                                 <  1:105414‑M1/257‑1 (MQ=255)
                                                                                                                                                                                                                                                       gctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCNTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGccc                                                 <  1:444466‑M1/257‑1 (MQ=255)
                                                                                                                                                                                                                                                       gctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACNTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGccc                                                 <  2:493710‑M1/257‑1 (MQ=255)
                                                                                                                                                                                                                                                       gctgcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcacggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagccc                                           <  1:626602‑M2/300‑258 (MQ=255)
                                                                                                                                                                                                                                                         tgcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcntcatcaaacttgccgaccgtacccaaaacatgcgcacgctgggctcacttcgcccggacaaacgtcgacgcatcgcccgtgaaactctcgaaatttatagcccgc                                         <  2:578937‑M2/300‑260 (MQ=255)
                                                                                                                                                                                                                                                         tgcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgc                                         <  1:181880‑M2/300‑260 (MQ=255)
                                                                                                                                                                                                                                                         tgcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcacgtgaaactctcgaaatttatagcccgc                                         <  2:97946‑M2/300‑260 (MQ=255)
                                                                                                                                                                                                                                                         tgcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgcagagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataaggaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgaccgtgaaactctcgaaatttatagcccgc                                         <  1:181813‑M2/300‑260 (MQ=255)
                                                                                                                                                                                                                                                          gcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCNACCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATCGCCCGCt                                              <  2:492922‑M1/260‑1 (MQ=255)
                                                                                                                                                                                                                                                          gcATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgct                                        <  1:496857‑M2/300‑261 (MQ=255)
                                                                                                                                                                                                                                                               aCGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgacgcatcgcccgtgaaactatagaaatttatagcccgctggcgc                                   <  1:434332‑M2/300‑266 (MQ=255)
                                                                                                                                                                                                                                                                    attgaagatactcccgccacctaccaggatCNGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGNGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGt                                    <  2:456902‑M1/270‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aTTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgt                              <  1:39230‑M2/300‑271 (MQ=255)
                                                                                                                                                                                                                                                                       gaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGANATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCCCGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTATAGAAATTTATAGCTCGCTGGCGCACCGTTTa                                 <  2:153772‑M1/273‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCACGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAg                                <  1:600238‑M1/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCACGGACAAACGTCGCCGCATCGCCCGTGAAACTATCGAAATTTATAGCACGCTGGCGCACCGTTTAg                                <  2:3378‑M1/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aaGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttag                          <  1:6309‑M2/300‑275 (MQ=255)
                                                                                                                                                                                                                                                                         aGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttagg                         >  2:6306‑M2/1‑25 (MQ=255)
                                                                                                                                                                                                                                                                         aGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgagggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttagg                         >  2:18157‑M2/1‑25 (MQ=255)
                                                                                                                                                                                                                                                                           atactcncgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGNCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTa                             >  2:552022‑M1/24‑300 (MQ=255)
                                                                                                                                                                                                                                                                           aTACTCCCGCCACCT‑AC‑‑‑‑‑TGGGATcgggatagggaacagctgtttggtaaaagcgtcgccgagctggtatagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgctgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggta                       >  2:91147‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                           aTACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatngcgatggtgcaggatatccgcgtcatnctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggta                       >  2:443765‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                           aTACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggta                       >  2:505913‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                           aTACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctgggagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgtgctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggta                       >  2:92575‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                              ctcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATcc                          >  1:562717‑M1/21‑300 (MQ=255)
                                                                                                                                                                                                                                                                                    ccACCT‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggtatccaccaca              <  1:281816‑M2/300‑287 (MQ=255)
                                                                                                                                                                                                                                                                                     cacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACAt                   <  1:664576‑M1/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      aCCT‑AA‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcacgctggcgcaccgtttaggtatccaccacatt            <  1:330450‑M2/300‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         t‑AC‑‑‑‑‑CAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggtatccaccacattaaa         <  2:595402‑M2/300‑292 (MQ=255)
                                                                                                                                                                                                                                                                                                    ggatcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggtatccaccacattaaaaccga    <  2:288154‑M2/300‑297 (MQ=255)
                                                                                                                                                                                                                                                                                                    ggatcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaaattccgcgataagaaagaggcgccggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctaatcaaacttgccgaccgtacccacaacatgcgcacgctgggcgcccttcgcccgaacaaacgtcgccacatcgaacgtgaaaccctcgaaatttatagcacgctggcgaaccgtttaggtatccaccacactaaaaccga    <  2:177977‑M2/300‑297 (MQ=255)
                                                                                                                                                                                                                                                                                                    ggATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGAGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCACGACGATTATGGCGATGGTGCAGGACCTCCGCGTCATCCTCATCAAACTTGCCGNCCGTACCCACAACATACGCACACTGGGCTCACTTCGAACGGACAAAAGTCGCCGCATCGCCAGTGAAACTCTCAAACCTTATAGCCCGCTGGTGCACCGTTTCGGTAGCCACCACATTAAAACCGAACTCGa    >  2:159912/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                      atatGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCNATGGTGCAGGATATCCGCGTCATCCTNATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCACGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAag  >  2:442807/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                          
TTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCT‑AC‑‑‑‑‑CAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAG  >  NC_000913/3822362‑3822949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.