Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,613,774	C→T	V36V (GTC→GTT)	nikA →	Ni(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,613,774	0	C	T	100.0%	312.2 / NA	87	V36V (GTC→GTT)	nikA	Ni(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/38);  total (49/38)

CTGCACCCCTACTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAC  >  NC_000913/3613493‑3614069                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        cTGCACCCCTACTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCAc                                                                                                                                                                                                                                                                                       >  1:353601/1‑300 (MQ=255)      ccccTACTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTctgtctcttatacacatctcgcag                                                                                                                                                                                                                                                                                  >  2:283389/1‑277 (MQ=255)         ctattCCCTTCACGCTCACCGCCGACAGTGTGCAATGGATCGATTCGGTTAACTAATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCTCCGCACTCTATTGGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTacac                                                                                                                                                                                                                                                                               <  2:59797/296‑1 (MQ=255)         cTACTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTacac                                                                                                                                                                                                                                                                               <  1:54173/300‑1 (MQ=255)         cTACTCCATTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCATTTAACTGATCCGCCCACCCGACTGCCCATCGATTGATCCAGAACAGTTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCTATGCCGCTGCACCAGATGAAATCACCACCGCCTTGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTacac                                                                                                                                                                                                                                                                               <  1:51126/300‑1 (MQ=255)         cTACTCCATTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTTGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTacac                                                                                                                                                                                                                                                                               <  1:457521/300‑1 (MQ=255)           aCTCCCTTTACGCTCACTTCCGACAGTGTGCAAGGGATCGATTCAGTTAACTGATCCGCCCACCCGACTACCCATCTATTGATCCAGAACAGGGAATCAGGAGGACGAAGACTGAAAATCGTCATACTTATTTCCTCCATCTATTTTAATCCATGGGGGTGACCATGCTCTCCACACCCCGCCGCACTCTATTGGCGCTGCTGGCTTGGGCGTTTTGTAGCGTCCATGCCGCTGCACCAGATGAAATCACAACCGCTTGGCTGGTGAATGGTGGGCCACTAAACCCACACCTTTACACGc                                                                                                                                                                                                                                                                             <  1:38048/300‑1 (MQ=255)            gtCCCTTTACGTTGACCGCCGACAGTGTGCAATGGATTGATTCAGTTAACTGATCCGCCCACCCGACTGTCCATCTATTGATCCAGGACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCGTGCTCTCCCCACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGGAATCACCACAGCCTGGCCGGTGAATGTTAGGCCACTAAACCCGCACCTTTACACGcc                                                                                                                                                                                                                                                                            >  2:163825/2‑300 (MQ=255)            cTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATNCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGANATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGcc                                                                                                                                                                                                                                                                            <  2:708616/300‑1 (MQ=255)               ccTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTaa                                                                                                                                                                                                                                                                         >  1:672322/1‑300 (MQ=255)                                  cAGTGTGCAATGGATCGATTCATTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGc                                                                                                                                                                                                                                                      <  2:455287/300‑1 (MQ=255)                                  cAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAANGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCNCAGAGc                                                                                                                                                                                                                                                      >  2:616763/1‑300 (MQ=255)                                    gtgtGCAATGGATCGATTCAGTTAACTGATCNGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAANCCATTGGGGTTACCATGCTCTCCACAGTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCAACACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCCGAGCAt                                                                                                                                                                                                                                                    >  2:525642/1‑300 (MQ=255)                                    gtgtGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCAt                                                                                                                                                                                                                                                    >  1:67641/1‑300 (MQ=255)                                      gtgCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATgg                                                                                                                                                                                                                                                  <  1:336156/300‑1 (MQ=255)                                        gtaaTGGATCGATTCCGTTACCTGCTCCGCCCCCCCGACTGCCCATCTATTGATCCAGAACAAGTAATCAGTATGACGAATACTTCCCATCGTCATACGTATTTCCGCCATCTATTTTAATCACTTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTCTCGTCCACGCAGCTACACCAGATAAAATCAACACCGCCTGGACGGTGAATGTTGGTCCACTACACCAGCACCTTTACCCTCCTAACTAGATGTTTGCACagagtctgggt                                                                                                                                                                                                                                                >  2:399533/3‑293 (MQ=255)                                               aTCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAAc                                                                                                                                                                                                                                         <  2:465927/300‑1 (MQ=255)                                                      ccgttcaCTGATCCGCCCACCCGACTGCCCATCCATCGATCCAGCACAGGTAATCAGTATGACGAATACTCAAAATCATCATACTTATTTCCGCCATCTATTTTAATCCATTGGTGTTACTATGCTCTCCACACTCCGCCGCACTTTATTTTCGCTGCTGACTTGTTTGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCAGTGAATGTTGGGCCACTACACCCGCACCTTTACCGGCCTAACCAGATGTTCACCCAGAGCATGGTTTATGCACCATTGGt                                                                                                                                                                                                                                  >  2:297300/7‑300 (MQ=255)                                                                         cccGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGt                                                                                                                                                                                                               >  1:334193/1‑300 (MQ=255)                                                                                gCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGa                                                                                                                                                                                                        <  2:20077/300‑1 (MQ=255)                                                                                gCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTNGCGCTGCTGGCTTGTGCGTCTTTTATNGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGa                                                                                                                                                                                                        >  2:497974/1‑300 (MQ=255)                                                                                 cccATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGAt                                                                                                                                                                                                       >  2:102603/1‑300 (MQ=255)                                                                                   cctCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCTTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATTGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATcc                                                                                                                                                                                                     <  2:304771/298‑1 (MQ=255)                                                                                   cATCTATGGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATcc                                                                                                                                                                                                     <  2:360610/300‑1 (MQ=255)                                                                                      cTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCAGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGt                                                                                                                                                                                                  >  1:8863/1‑300 (MQ=255)                                                                                            aTCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGNCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg                                                                                                                                                                                            >  2:665457/1‑300 (MQ=255)                                                                                            aTCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg                                                                                                                                                                                            >  1:354496/1‑300 (MQ=255)                                                                                                 gAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaa                                                                                                                                                                                       >  2:166703/1‑300 (MQ=255)                                                                                                    cAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGc                                                                                                                                                                                    >  1:469124/1‑300 (MQ=255)                                                                                                    cAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTACACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATTAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGc                                                                                                                                                                                    >  2:278705/1‑300 (MQ=255)                                                                                                      ggTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTg                                                                                                                                                                                  >  1:238271/1‑300 (MQ=255)                                                                                                      ggTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCAATAAACCCGCACCTTTACACGCCTAACCAGCTGTTCGCCCAGAGCATGGTTTATGAACNATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCAGTGGCTGGCAAAAAGCTg                                                                                                                                                                                  >  2:564462/1‑300 (MQ=255)                                                                                                              gTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTNGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCCTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACAATTGGTGNAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCAtt                                                                                                                                                                          >  2:706941/1‑300 (MQ=255)                                                                                                              gTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCAtt                                                                                                                                                                          >  1:331344/1‑300 (MQ=255)                                                                                                                                    tCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTTGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGAc                                                                                                                                                    <  2:241411/300‑1 (MQ=255)                                                                                                                                    tCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGAc                                                                                                                                                    <  1:727914/300‑1 (MQ=255)                                                                                                                                        aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTc                                                                                                                                                <  1:183974/300‑1 (MQ=255)                                                                                                                                            attgccGCCATCTATTGTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGNACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAccc                                                                                                                                            <  2:674388/296‑1 (MQ=255)                                                                                                                                            aTTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAccc                                                                                                                                            <  2:288772/300‑1 (MQ=255)                                                                                                                                            aTTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAccc                                                                                                                                            <  1:247913/300‑1 (MQ=255)                                                                                                                                            aTTTACGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAccc                                                                                                                                            <  1:418305/300‑1 (MQ=255)                                                                                                                                                      tCTATTTTAATCCATTTGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCNCCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGtgatga                                                                                                                                  <  1:513201/300‑1 (MQ=255)                                                                                                                                                      tCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGtgatga                                                                                                                                  <  2:330759/300‑1 (MQ=255)                                                                                                                                                         aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGTTGGCTTGTGCGTCTTTTATCGTACATGCCGCTGCACCAGATGAAATCAACAACGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTTGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGATTCGGTGATCCCGTGGCTGGCAAAAAGCTGGATTCCTTCAGAAGCTGGTAAAACCTGGACCTTCACCCTGTGTGATGACGt                                                                                                                               >  2:77072/1‑300 (MQ=255)                                                                                                                                                                      ggggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCCTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGAAGGTTCGGTGATCCCGTGGCTGGCAAACAGCTGGAATCATTCAGAAGACGGTCAAACCTGGAACTTCACCCTGCGTGATGAAGTGAAATTCTCCAAc                                                                                                                  >  2:411929/1‑300 (MQ=255)                                                                                                                                                                        ggTTACCATGCTCTCCACACTCCGCCGCACTTTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACgg                                                                                                                <  2:71452/300‑1 (MQ=255)                                                                                                                                                                            aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGATGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAAACTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGaa                                                                                                            >  2:64183/1‑300 (MQ=255)                                                                                                                                                                            aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCAGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGaa                                                                                                            >  2:139870/1‑300 (MQ=255)                                                                                                                                                                            aCCATGCTCTCCACACTCCGCCGCACTCTATNTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCNGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGaa                                                                                                            >  2:537987/1‑300 (MQ=255)                                                                                                                                                                                 gCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGtt                                                                                                       >  1:352122/1‑300 (MQ=255)                                                                                                                                                                                   tctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCg                                                                                                     >  1:145871/1‑300 (MQ=255)                                                                                                                                                                                   tctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCg                                                                                                     >  2:433934/1‑300 (MQ=255)                                                                                                                                                                                   tctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCAGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCg                                                                                                     >  1:202099/1‑300 (MQ=255)                                                                                                                                                                                   tctcCACACTCCGCCGCACTCTATTTGCGCCGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAACCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCg                                                                                                     >  1:498434/1‑300 (MQ=255)                                                                                                                                                                                    ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAACCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGa                                                                                                    >  1:26745/1‑300 (MQ=255)                                                                                                                                                                                    ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTAGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGa                                                                                                    >  1:605863/1‑300 (MQ=255)                                                                                                                                                                                            tCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCANGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAANTTCTCCAACGGTGAACCGTTCGATGCCGAgg                                                                                            <  2:541675/300‑1 (MQ=255)                                                                                                                                                                                             ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCAACCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAggc                                                                                           >  1:386975/1‑300 (MQ=255)                                                                                                                                                                                                     ctctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAg                                                                                   >  2:678053/1‑300 (MQ=255)                                                                                                                                                                                                       ctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGaa                                                                                 >  1:354300/1‑300 (MQ=255)                                                                                                                                                                                                       ctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCAGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGaa                                                                                 >  1:354344/1‑300 (MQ=255)                                                                                                                                                                                                       ctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCATAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCNGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGaa                                                                                 >  2:174405/1‑300 (MQ=255)                                                                                                                                                                                                                ctgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAANCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCcgc                                                                        >  1:520993/1‑300 (MQ=255)                                                                                                                                                                                                                ctgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCcgc                                                                        >  1:46722/1‑300 (MQ=255)                                                                                                                                                                                                                     ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGt                                                                   >  1:668631/1‑300 (MQ=255)                                                                                                                                                                                                                       cTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGc                                                                 >  1:487638/1‑300 (MQ=255)                                                                                                                                                                                                                            gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGTGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGAt                                                            <  2:493252/300‑1 (MQ=255)                                                                                                                                                                                                                            gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGNCGGTTCGGTGATCCCGTGGCTGGCAANAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCTTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGAt                                                            <  2:520591/300‑1 (MQ=255)                                                                                                                                                                                                                            gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAANAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGNGGCGGCAGAAAACTTCCGCGCAGTGCTCGAt                                                            <  2:533659/300‑1 (MQ=255)                                                                                                                                                                                                                            gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGAt                                                            <  1:10622/300‑1 (MQ=255)                                                                                                                                                                                                                            gCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCACCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGTTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGAt                                                            <  2:287588/300‑1 (MQ=255)                                                                                                                                                                                                                                      tCGTCCNTGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGNGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAAc                                                  <  2:635599/300‑1 (MQ=255)                                                                                                                                                                                                                                      tCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAAc                                                  <  1:140643/300‑1 (MQ=255)                                                                                                                                                                                                                                         tCCATGCTGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGTTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTc                                               <  2:310822/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGGTCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAAACTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCAAGCctggctg                                    >  2:38993/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ccAGATGAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGANATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAg                                 <  1:509704/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gAAATCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCa                           >  2:492409/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aaTCACCACCGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCaaa                         >  1:313303/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   accaccGCCTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAg                     >  1:640545/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ccTGGCCGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGAttgttg              <  1:129091/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               ccGGTGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCAAGCCTGGCTGGAGTTGGCAAACCAGATTGTTGATGtt         >  2:241960/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tGAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCTTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAg     <  2:373217/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ggAATGTTGGGCCACGAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGTTTTATGAACCATTGTTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAg     <  2:290150/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gAATGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGc    <  2:51915/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aTGTTGGGCCACTAAACCCGCACCTTTACACTCCTAACCAGATGTTCGCCCATAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCNGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGAGGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCGGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAc  <  2:658884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aTGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCNGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAc  <  2:658874/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aTGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAc  >  1:162042/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aTGTTGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAc  >  2:684862/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        CTGCACCCCTACTCCCTTTACGCTCACCGCCGACAGTGTGCAATGGATCGATTCAGTTAACTGATCCGCCCACCCGACTGCCCATCTATTGATCCAGAACAGGTAATCAGTATGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGGACTCATTCAGAAGATGGTAAAACCTGGACCTTCACCCTGCGTGATGACGTGAAATTCTCCAACGGTGAACCGTTCGATGCCGAGGCGGCGGCAGAAAACTTCCGCGCAGTGCTCGATAACCGTCAACGTCACGCCTGGCTGGAGCTGGCAAACCAGATTGTTGATGTTAAAGCAC  >  NC_000913/3613493‑3614069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑