Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,114,752	G→A	A1454V (GCC→GTC)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,114,752	0	G	A	100.0%	217.8 / NA	60	A1454V (GCC→GTC)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/33);  total (27/33)

CCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCT  >  NC_000913/3114458‑3115047                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        cccGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGAcc                                                                                                                                                                                                                                                                                                    >  1:733753/1‑300 (MQ=255)       aaGCAGGCGCATCGGGCAAATGTGTTAACCTGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCaggag                                                                                                                                                                                                                                                                                              <  1:167747/300‑1 (MQ=255)       aaGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTANGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCaggag                                                                                                                                                                                                                                                                                              <  2:665897/300‑1 (MQ=255)       aaGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCaggag                                                                                                                                                                                                                                                                                              <  2:215739/300‑1 (MQ=255)             gcgcATCGGGCAAATGTGTTAACCCTGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCTGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGTAGGcacac                                                                                                                                                                                                                                                                                        <  2:117168/300‑1 (MQ=255)                       cAAATGTGTTAACCCTGTGCACCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTGCTTACTCTGCATACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGTTCCCTGTTCCGGTTTCGGCAGATCGATTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCg                                                                                                                                                                                                                                                                              <  2:66195/300‑1 (MQ=255)                       cAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGTAGGCACACAGCATCAGCg                                                                                                                                                                                                                                                                              <  2:285158/300‑1 (MQ=255)                       cAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCg                                                                                                                                                                                                                                                                              <  2:739995/300‑1 (MQ=255)                       cAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGACCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTNCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTNACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCg                                                                                                                                                                                                                                                                              <  2:602623/300‑1 (MQ=255)                            gtgtTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTcc                                                                                                                                                                                                                                                                         >  1:590317/1‑300 (MQ=255)                            gtgtTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCANCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTcc                                                                                                                                                                                                                                                                         >  1:512742/1‑300 (MQ=255)                                           gcCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTCAAGAACTCCGAAAGATCNGTCTGGACGCCCCAGGAGGCACACAGCATAAGCGTGTCCACTGCGTTNCCGTTg                                                                                                                                                                                                                                                          >  2:620306/1‑300 (MQ=255)                                            ccTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTgg                                                                                                                                                                                                                                                         >  2:689031/1‑300 (MQ=255)                                            ccTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTgg                                                                                                                                                                                                                                                         >  1:757903/1‑300 (MQ=255)                                                  ttCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTccctgtctcttatacacatctcgc                                                                                                                                                                                                                                                   <  1:55895/300‑23 (MQ=255)                                                             tGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTc                                                                                                                                                                                                                                        <  1:608410/300‑1 (MQ=255)                                                                ggcgCGAGCGCTTTATCCGGCCTACGTGCTTACTCGGCAGACATCTTAGGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGNGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTNGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCTAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTg                                                                                                                                                                                                                                     <  2:601558/300‑1 (MQ=255)                                                                         gcCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAAc                                                                                                                                                                                                                            >  2:376276/1‑300 (MQ=255)                                                                              aTCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTc                                                                                                                                                                                                                       <  1:627885/300‑1 (MQ=255)                                                                                               aCTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATcgc                                                                                                                                                                                                      <  1:585213/300‑1 (MQ=255)                                                                                                              tATGCTCGGTAACCTGATTAATGGTTTCCGTTCCCTGTTTCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGAt                                                                                                                                                                                       <  2:260445/300‑1 (MQ=255)                                                                                                                    cGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCa                                                                                                                                                                                 >  1:297620/1‑300 (MQ=255)                                                                                                                    cGGTAACCCGATTAATGGTTTCCGGTCCCTGTTCCGGTCTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTAGAAGCTCATCTCGCTCGCCCCCGGCAGCTGNTAAGCATTCGCGCCCGGATTCCATTTNTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCCGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGACGCGTGCTTTACGATGCATCa                                                                                                                                                                                 >  2:523659/1‑300 (MQ=255)                                                                                                                            tGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAAc                                                                                                                                                                         <  1:135451/300‑1 (MQ=255)                                                                                                                                     gTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAg                                                                                                                                                                >  1:677644/1‑300 (MQ=255)                                                                                                                                      tttCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGc                                                                                                                                                               <  1:287667/300‑1 (MQ=255)                                                                                                                                        tCCGGTCCCTGTTCCGGTGTCGGCAGATCGATTGACGCGAGCGTGTTGTAAGCCNACTGGCTCACACCGCCCTCGAAGCTCACCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCGTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCt                                                                                                                                                             <  2:555978/300‑1 (MQ=255)                                                                                                                                                cTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTACGCTGCGTTACCGTTGGATTCAGCACAGTAGTTCTTGCTGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTTCATCCTTTCATccc                                                                                                                                                     >  2:3998/1‑300 (MQ=255)                                                                                                                                                                 gATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTaaa                                                                                                                                    >  1:697898/1‑300 (MQ=255)                                                                                                                                                                   tCGATTGACGCGAGCGTGTTGTAATCCGACTGGCTCACACCGCCCTCTAAGCTCATCTCGCTCGCCCCCTGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTCTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAAc                                                                                                                                  <  2:388171/300‑1 (MQ=255)                                                                                                                                                                      aGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAActct                                                                                                                               >  1:492012/1‑300 (MQ=255)                                                                                                                                                                           cgcgAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCag                                                                                                                          <  1:81420/300‑1 (MQ=255)                                                                                                                                                                               aGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAAATGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAgagga                                                                                                                      >  1:42750/1‑300 (MQ=255)                                                                                                                                                                                    gttgtAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGcc                                                                                                                 >  1:9826/1‑300 (MQ=255)                                                                                                                                                                                    gttgtAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGca                                                                                                                 >  1:89076/1‑299 (MQ=255)                                                                                                                                                                                              gACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATAc                                                                                                       >  1:293101/1‑300 (MQ=255)                                                                                                                                                                                                  ggCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATNAGCGTGTCCGCTGCGTTACCGTTGGANTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                                                   >  2:507026/1‑300 (MQ=255)                                                                                                                                                                                                  ggCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTNACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                                                   >  1:558096/1‑300 (MQ=255)                                                                                                                                                                                                  ggCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                                                   >  1:105948/1‑300 (MQ=255)                                                                                                                                                                                                  ggCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCAtt                                                                                                   >  2:53466/1‑300 (MQ=255)                                                                                                                                                                                                  ggCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGACACCAtt                                                                                                   >  2:4179/1‑300 (MQ=255)                                                                                                                                                                                                                        cATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATCTCTTGATATCAAAGTTTTtctc                                                                             >  2:324639/1‑300 (MQ=255)                                                                                                                                                                                                                                    ccccGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCTAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCATAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCtt                                                                 <  2:494231/300‑1 (MQ=255)                                                                                                                                                                                                                                        ggCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGc                                                             <  1:2333/300‑1 (MQ=255)                                                                                                                                                                                                                                        ggCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGc                                                             <  2:687011/300‑1 (MQ=255)                                                                                                                                                                                                                                            gCTGGTAAGCATTCGCGCCCGGATTCCATTTNTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTNTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCtgtg                                                         >  2:540941/1‑300 (MQ=255)                                                                                                                                                                                                                                              tGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCATTCAGCTGTGCg                                                       >  1:301066/1‑300 (MQ=255)                                                                                                                                                                                                                                               gggaaGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                                      <  1:761015/297‑1 (MQ=255)                                                                                                                                                                                                                                               ggTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt                                                      <  1:572820/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaGCATTCGCGCCCGTATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa                                                   <  2:211467/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa                                                   <  1:299006/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaGCATNCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGANGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa                                                   <  2:616237/300‑1 (MQ=255)                                                                                                                                                                                                                                                         cgcgCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCANGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCANTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcagcag                                            <  2:697416/300‑1 (MQ=255)                                                                                                                                                                                                                                                         cgcgCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGTAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcagcag                                            <  2:242011/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cccGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg                                        >  2:371135/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ccATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGATGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc                                <  2:134584/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     ccATTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc                                <  1:53633/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       aTTTCTTAAAGAACTCCGAAAGATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAc                              <  1:570526/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gATCCGTCTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgt         >  1:499346/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   cTGGACGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGct  <  1:375820/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        CCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAGCACAGTAATTCTTGCCGCCAAACTTGTCATTGCTGACCTCATCGCCGCGTGCTTTACGATGCATCAACTGGAACAGGTTCCAGCCTTTCATCCCTTCACGCTCGCTGTAAAACTCTGGCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCT  >  NC_000913/3114458‑3115047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑