BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,360,740	G→A	P57S (CCT→TCT)	puuA ←	glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,360,740	0	G	A	100.0%	353.0 / NA	100	P57S (CCT→TCT)	puuA	glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (67/33); total (67/33)

Only 100 of 101 total aligned reads displayed.

GACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGACCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACC  >  NC_000913/1360444‑1361033

gttgtagagttgtgattcatacaggctgagttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTGCGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAAGAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAAt                                                                                                                                                                                                                                                                                                    >  2:653679‑M2/210‑300 (MQ=255)

gttgtagagttgtgattcatacaggctgagttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAAt                                                                                                                                                                                                                                                                                                    >  1:329706‑M2/210‑300 (MQ=255)

gttgtagagttgtgattcatacaggctgagttcacgggctgcggcagtaacaacgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCCTTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAAt                                                                                                                                                                                                                                                                                                    >  2:338469‑M2/210‑300 (MQ=255)

        gttgtgattcatacaggctgagttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTc                                                                                                                                                                                                                                                                                            >  2:744060‑M2/202‑300 (MQ=255)

              actcatacaggctgagttcacgggttgcggcaggaacaccgatacgttcagcaagcttcagtgcttcactgcgaaattcaggcgaatgctgtttacgggggtttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCTAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGa                                                                                                                                                                                                                                                                                      <  2:230710‑M2/105‑1 (MQ=255)

                 catacaggctgagttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGc                                                                                                                                                                                                                                                                                   >  1:625772‑M2/193‑300 (MQ=255)

                     caggctgagttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCNTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGANGCAAcc                                                                                                                                                                                                                                                                               >  2:616275‑M2/189‑300 (MQ=255)

                            agttcaagggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTaaa                                                                                                                                                                                                                                                                        <  1:484647‑M2/119‑1 (MQ=255)

                             gttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttcgccgcgtgtccactattgctgggtaagatcaCTTCTAATACGTTGACCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAAt                                                                                                                                                                                                                                                                       >  2:581451‑M2/181‑300 (MQ=255)

                             gttcacgggctgcggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAAt                                                                                                                                                                                                                                                                       >  1:737357‑M2/181‑300 (MQ=255)

                                          ggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCANTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAAc                                                                                                                                                                                                                                                          >  2:668024‑M2/168‑300 (MQ=255)

                                          ggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagnttactcacttagccgcgtgtccactantgctgggtaagatcaCTTCTACTACGTTGCACAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAAGAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGtaaaaa                                                                                                                                                                                                                                                          >  2:496583‑M2/168‑299 (MQ=255)

                                          ggcagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggtttttgactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAAc                                                                                                                                                                                                                                                          <  2:300723‑M2/133‑1 (MQ=255)

                                            cagtaacaccgatgcgttcagcaagcttcagngcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagttnactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCCGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACAt                                                                                                                                                                                                                                                        >  2:548067‑M2/166‑300 (MQ=255)

                                            cagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACAt                                                                                                                                                                                                                                                        >  2:220057‑M2/166‑300 (MQ=255)

                                            cagtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACAt                                                                                                                                                                                                                                                        >  1:438016‑M2/166‑300 (MQ=255)

                                              gtaacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCNACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCa                                                                                                                                                                                                                                                      >  2:177413‑M2/164‑300 (MQ=255)

                                                aacaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgntgggtaagatcaCTTCTACTACGTTGNCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGTTAAGTAAAACATCAAc                                                                                                                                                                                                                                                    <  2:522981‑M2/139‑1 (MQ=255)

                                                 acaccgatgcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACa                                                                                                                                                                                                                                                   >  1:86071‑M2/161‑300 (MQ=255)

                                                  caccgatgcgttcagcaagcttcatggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACat                                                                                                                                                                                                                                                  <  1:314529‑M2/141‑1 (MQ=255)

                                                         gcgttcagcaagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGt                                                                                                                                                                                                                                           >  1:701621‑M2/153‑300 (MQ=255)

                                                            ttcagcaagcttcagggcttcactgcgaaattcatgcgaatgctgtttacggggntttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttacttacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGNTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTAtt                                                                                                                                                                                                                                        <  2:706430‑M2/151‑1 (MQ=255)

                                                                 caagcttcagggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcanttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGt                                                                                                                                                                                                                                   <  1:527038‑M2/156‑1 (MQ=255)

                                                                      ttcagggcttcactgcgaaattcatgcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAgcgc                                                                                                                                                                                                                              <  1:316220‑M2/161‑1 (MQ=255)

                                                                      ttcagggcttcactgcgaaatgcaggcgaatgctgtttacggggttttttactgnttgatactgtttttgttatgtgattcagctctgactgagagtttacttacttagccgcgtgtccactattgttgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGGGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGTAACCATTTGAATCGGGAAGTAAAACATCAACATATTGCGTAGTCGGGTAgcgc                                                                                                                                                                                                                              <  2:559932‑M2/161‑1 (MQ=255)

                                                                           ggcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAg                                                                                                                                                                                                                         <  1:77903‑M2/166‑1 (MQ=255)

                                                                            gcttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAgg                                                                                                                                                                                                                        >  1:129346‑M2/134‑300 (MQ=255)

                                                                            gcttcacggcgaaattcaggcgaatactatttacgtggttttttactggttgatactggttttgtcatgtgagtcacctctgactgagagtttacgcacttagccgcgtgtccactattgctggttaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACATAAATACGCTTTCCACGGGAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAgg                                                                                                                                                                                                                        <  2:15471‑M2/167‑1 (MQ=255)

                                                                             cttcactgcgaaattcatgcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt                                                                                                                                                                                                                       <  1:407265‑M2/168‑1 (MQ=255)

                                                                             cttcactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt                                                                                                                                                                                                                       <  1:132656‑M2/168‑1 (MQ=255)

                                                                             cttcactgcgaaattcaggcgaatgctatttactgggttttttactggttgatantgttttggtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactagtgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGTTAGCGCTCCAGGt                                                                                                                                                                                                                       <  2:557080‑M2/168‑1 (MQ=255)

                                                                             cttcactgcgaaatgcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGt                                                                                                                                                                                                                       <  2:119468‑M2/168‑1 (MQ=255)

                                                                             cttcacggcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTGTTCGGGTAGCGCTCCAGGt                                                                                                                                                                                                                       <  1:446824‑M2/168‑1 (MQ=255)

                                                                                cactgcgaaattcaggcgaatgctgtttacggggttttttactggttgatacggtttttgtcatgttagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCTGGAAATAACACCCTTTCTCGAGCTCCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGGAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGGAGc                                                                                                                                                                                                                    <  1:311906‑M2/171‑1 (MQ=255)

                                                                                  ctgcgaaattcagtcgaatgctgtttacggggttttttactggttgatactgttnttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTNAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt                                                                                                                                                                                                                  <  2:708102‑M2/173‑1 (MQ=255)

                                                                                  ctgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGt                                                                                                                                                                                                                  <  1:301134‑M2/173‑1 (MQ=255)

                                                                                  ctgcgaaattcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCACAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGTTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGgtagtgt                                                                                                                                                                                                                  >  2:289620‑M2/128‑297 (MQ=255)

                                                                                         attcaggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGNAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAAAAGAAACACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACCTCAACATATTGCGTATTCGGGTAGCGATACAGGTAGCGTTTCACtt                                                                                                                                                                                                           >  2:193638‑M2/121‑300 (MQ=255)

                                                                                              ggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGc                                                                                                                                                                                                      >  1:741622‑M2/116‑300 (MQ=255)

                                                                                              ggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGAGCTACAGGTAGCGTTTCACTTCCTGc                                                                                                                                                                                                      >  2:729114‑M2/116‑300 (MQ=255)

                                                                                              ggcgaatgctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCAGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCACCATATTGCGTATTCGGGTAGCGCTCAAGGTAGCGTTTCACTTCCTGc                                                                                                                                                                                                      >  1:501885‑M2/116‑300 (MQ=255)

                                                                                                     gctgtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAgg                                                                                                                                                                                               <  1:182061‑M2/192‑1 (MQ=255)

                                                                                                        gtttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAANTACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGgcgc                                                                                                                                                                                            >  2:673279‑M2/106‑300 (MQ=255)

                                                                                                        gtttaaggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCTTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCCTTTAAATCGGTAAGTAAAACATCAACANATTGCGTATTCGGGTAGCGCTCAAGGTAGCGTTTAACTTACTGCGTAAAGgcgc                                                                                                                                                                                            >  2:557658‑M2/106‑300 (MQ=255)

                                                                                                          ttacggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGNAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTa                                                                                                                                                                                          >  1:511362‑M2/104‑300 (MQ=255)

                                                                                                             cggggttttttactggttgatactgtttttgtcatgtgagtcacctctgactganagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAccc                                                                                                                                                                                       <  2:566641‑M2/200‑1 (MQ=255)

                                                                                                                 gttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGcc                                                                                                                                                                                   >  1:105409‑M2/97‑300 (MQ=255)

                                                                                                                  ttttttactggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCct                                                                                                                                                                                  <  1:463395‑M2/205‑1 (MQ=255)

                                                                                                                           ggttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGa                                                                                                                                                                         >  1:105935‑M2/87‑300 (MQ=255)

                                                                                                                             ttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGAct                                                                                                                                                                       >  2:728510‑M2/85‑300 (MQ=255)

                                                                                                                             ttgatactgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCACTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGAct                                                                                                                                                                       <  1:245912‑M2/216‑1 (MQ=255)

                                                                                                                                   ctgtttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGaa                                                                                                                                                                 >  2:246680‑M2/79‑300 (MQ=255)

                                                                                                                                      tttttgtcatgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAANACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACaa                                                                                                                                                              <  1:527746‑M2/225‑1 (MQ=255)

                                                                                                                                              atgtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTctct                                                                                                                                                      >  1:743051‑M2/68‑300 (MQ=255)

                                                                                                                                                gtgagtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTGCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTAc                                                                                                                                                    >  2:715773‑M2/66‑300 (MQ=255)

                                                                                                                                                   agtcacctctgactgagagtttcctcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGCAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGAGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTc                                                                                                                                                 >  2:731601‑M2/63‑300 (MQ=255)

                                                                                                                                                   agtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGANGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTc                                                                                                                                                 >  2:171765‑M2/63‑300 (MQ=255)

                                                                                                                                                   agtcacctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTc                                                                                                                                                 >  1:407693‑M2/63‑300 (MQ=255)

                                                                                                                                                       acctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACg                                                                                                                                             >  1:701239‑M2/59‑300 (MQ=255)

                                                                                                                                                        cctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGTGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGa                                                                                                                                            >  2:206027‑M2/58‑300 (MQ=255)

                                                                                                                                                        cctctgactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGa                                                                                                                                            >  1:231749‑M2/58‑300 (MQ=255)

                                                                                                                                                             gactgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTg                                                                                                                                       >  1:158158‑M2/53‑300 (MQ=255)

                                                                                                                                                              actgagagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCNTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTgg                                                                                                                                      <  1:527893‑M2/249‑1 (MQ=255)

                                                                                                                                                                   gagtttactcacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCAAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTcc                                                                                                                                 >  1:193333‑M2/47‑300 (MQ=255)

                                                                                                                                                                            cacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCACCGATATTGGTTTCCATGAttctt                                                                                                                        >  2:288291‑M2/38‑300 (MQ=255)

                                                                                                                                                                            cacttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGAttctt                                                                                                                        >  1:475112‑M2/38‑300 (MQ=255)

                                                                                                                                                                             acttagccgcgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGAttcttc                                                                                                                       >  1:399106‑M2/37‑300 (MQ=255)

                                                                                                                                                                                      cgtgtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGgt                                                                                                              >  1:199020‑M2/28‑300 (MQ=255)

                                                                                                                                                                                         gtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGNTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCCTGATTCTTCGCCTTTGgtttg                                                                                                           >  1:511511‑M2/25‑300 (MQ=255)

                                                                                                                                                                                         gtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGgtttg                                                                                                           >  1:490485‑M2/25‑300 (MQ=255)

                                                                                                                                                                                         gtccactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTATGAATGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGgtttg                                                                                                           >  1:59393‑M2/25‑300 (MQ=255)

                                                                                                                                                                                            cactattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCCACCTCAACAACACTGGTTTCCATGATTCTTCGCCTTTGgcttgttt                                                                                                        >  2:581901‑M2/22‑300 (MQ=255)

                                                                                                                                                                                              ctattgctgggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTc                                                                                                      >  1:760975‑M2/20‑300 (MQ=255)

                                                                                                                                                                                                      gggtaagatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGNGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTANTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGtt                                                                                              >  2:704412‑M2/12‑300 (MQ=255)

                                                                                                                                                                                                           agatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCaa                                                                                         <  1:692060‑M2/294‑1 (MQ=255)

                                                                                                                                                                                                            gatcaCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCNTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCaaa                                                                                        >  1:552221‑M2/6‑300 (MQ=255)

                                                                                                                                                                                                                 cttctACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACGCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTa                                                                                   >  2:478230/1‑300 (MQ=255)

                                                                                                                                                                                                                 cttctACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGTGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAACCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTa                                                                                   >  2:70033/1‑300 (MQ=255)

                                                                                                                                                                                                                   tctACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTaaa                                                                                 >  1:604698/1‑300 (MQ=255)

                                                                                                                                                                                                                    ctactaCGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTaaaa                                                                                >  2:241654/1‑300 (MQ=255)

                                                                                                                                                                                                                    ctactaCGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTaaaa                                                                                >  1:396115/1‑300 (MQ=255)

                                                                                                                                                                                                                           gTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTAACCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATg                                                                         >  2:254574/1‑300 (MQ=255)

                                                                                                                                                                                                                                   gAATATCCATTGCAAATACCGAGGCCGGGAANTAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATANTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGAACACCAt                                                                 >  2:552606/1‑300 (MQ=255)

                                                                                                                                                                                                                                   gAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCAt                                                                 >  1:287910/1‑300 (MQ=255)

                                                                                                                                                                                                                                   gAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCAAAGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTAGCATTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCAt                                                                 >  2:223566/1‑300 (MQ=255)

                                                                                                                                                                                                                                        tCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGNTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCNTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGaa                                                            <  2:705113/300‑1 (MQ=255)

                                                                                                                                                                                                                                        tCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGaa                                                            <  1:761760/300‑1 (MQ=255)

                                                                                                                                                                                                                                        tCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGaa                                                            <  2:723352/300‑1 (MQ=255)

                                                                                                                                                                                                                                        tCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGGAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGaa                                                            <  1:761752/300‑1 (MQ=255)

                                                                                                                                                                                                                                          cATTGCAAATACCGAGGCCGTGAAATAACACCCTTCCTCGAGCTTCTTCAGGCTTGAAACAGAAGTGCGCTTTCCACGGAAGCAACCATTTAAATTGGTAAGTGAAACATCAACATATTGCTTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATc                                                          <  2:423839/300‑1 (MQ=255)

                                                                                                                                                                                                                                           aTTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCt                                                         >  1:169205/1‑300 (MQ=255)

                                                                                                                                                                                                                                           aTTGCAAATACCGAGGCCGGGAAATAACACCCTTTCGCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCt                                                         <  2:480846/300‑1 (MQ=255)

                                                                                                                                                                                                                                                 aaTACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGc                                                   <  1:342157/300‑1 (MQ=255)

                                                                                                                                                                                                                                                              ggAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGttt                                      >  1:567009/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                aaaTAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGt                                    >  1:265389/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                        cccTTTCTCGAGCTTCTTCAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTCCCCCGCCTCTCTTCTGACTGCTGACCAAAGTTCTCTAACTCAACGATATTGGTTTCCATGATTCTTAGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAACATATAGTGACCACCATTCGAATCTGTATGCAAACTAAATGCTTGTCAAACGtt                            >  2:211197/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                     ttcttcAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGc               >  2:405619/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                      tcttcAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCa              >  1:308713/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                      tcttcAGGCTTGAAACAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTGCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCANCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCa              >  2:561146/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                                  aaaCAGAAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAAcc  >  1:621239/1‑300 (MQ=255)

GACCGGGAACAATCCGCGCTGGCGCAGCTGCTGCCACAGGCGGTTAAGAACGTTCCGCGGCTCAACGTCAAAGGGAGCGCCATCTTCATCGACCATGGTCAGGAGCATCTGACCGATAAACTCTGGATCGGCGGCAGAAGGAGTTAAGGAACCGAGAACAGGAACACAGGTACGATCCGGCTCGCCCATTTCCTGACCCAGACCTGCTTCTTCTACTACGTTGCCCAGAATATCCATTGCAAATACCGAGGCCGGGAAATAACACCCTTTCTCGAGCTTCTTCAGGCTTGAAACAGGAATACGCTTTCCACGGAAGCAACCATTTAAATCGGTAAGTAAAACATCAACATATTGCGTATTCGGGTAGCGCTCCAGGTAGCGTTTCACTTCCTGCGTAAAGGCGCTACCCCGCCTCTCTTCTGACTGCTGAACAAAGTTCTCTACTTCAACGATATTGGTTTCCATGATTCTTCGCCTTTGGTTTGTTTTCCGCTCGTTATCAAAGCGTAAAATATAATGACCACCATTCGAATCTGTATGCAAACTAAATGTTTGTCAAATGTTAAATTGAGTTTGCAAAAATGAAAACC  >  NC_000913/1360444‑1361033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`