Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,277	G→A	R188H (CGT→CAT)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,277	0	G	A	100.0%	210.9 / NA	61	R188H (CGT→CAT)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/33);  total (28/33)

TTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGT  >  NC_000913/276989‑277564                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                ttGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGt                                                                                                                                                                                                                                                                                      <  1:639403/300‑1 (MQ=255)                aGCTGGCGCGTAAAGCCCTTTAAGCGTACCTTGCAGAGAACCCGCAGGCGGGGACGCTTTTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGTCGATTATCATTGTAGCGTTGAGGCATTTCAGTCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                       <  2:390748/300‑1 (MQ=255)                aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                       <  2:724398/300‑1 (MQ=255)                aGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                                                                       <  1:336268/300‑1 (MQ=255)                    ggCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTTCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTgcg                                                                                                                                                                                                                                                                   >  1:608608/1‑300 (MQ=255)                    ggCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTgcg                                                                                                                                                                                                                                                                   >  2:255436/1‑300 (MQ=255)                        cgTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGAc                                                                                                                                                                                                                                                               >  1:697835/1‑300 (MQ=255)                                       gCGTATCTGGCAGAGAACCCGCAGGCTGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGACTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGATGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGc                                                                                                                                                                                                                                                <  2:199833/300‑1 (MQ=255)                                       gCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGc                                                                                                                                                                                                                                                <  1:39856/300‑1 (MQ=255)                                         gTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGa                                                                                                                                                                                                                                              >  2:446084/1‑300 (MQ=255)                                         gTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCACGCGCGTGGAAGCCTTGCTGGATGCCGNGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGTTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCCTTTACCCTGCGCGACGGCGAACACCCGAGCGa                                                                                                                                                                                                                                              >  2:187198/1‑300 (MQ=255)                                          tATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGNCTTACGGCGCGTATCTGGCGGATGGCTGTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGAc                                                                                                                                                                                                                                             <  2:571211/300‑1 (MQ=255)                                           aTCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACg                                                                                                                                                                                                                                            >  1:487806/1‑300 (MQ=255)                                                 cAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgc                                                                                                                                                                                                                                      <  1:571309/300‑1 (MQ=255)                                                  agagAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTAcgcc                                                                                                                                                                                                                                     <  2:300459/300‑1 (MQ=255)                                                     gaACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTACGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCt                                                                                                                                                                                                                                  >  2:313885/1‑300 (MQ=255)                                                              ggCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGt                                                                                                                                                                                                                         >  1:710572/1‑300 (MQ=255)                                                                        cTTCTGGTGGCGGGATCCGTCGGGCCTGACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGgttgcgttg                                                                                                                                                                                                               <  2:354157/300‑1 (MQ=255)                                                                                      aTCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATcc                                                                                                                                                                                                 <  1:103896/300‑1 (MQ=255)                                                                                                         gcgTATCTTGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGc                                                                                                                                                                              <  2:409412/300‑1 (MQ=255)                                                                                                             aTCTGGCGGATGGCTCCGAATCCCATGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCATTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCATGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGTGCGGGCGTGGTTCTCCTTTACCCTGCGCGACAGTGAACACCTGAGCGACGGTACGCCGCGGCGTGACGTGGTTGCATTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTa                                                                                                                                                                          >  2:248363/1‑300 (MQ=255)                                                                                                               cTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCTTGGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAAc                                                                                                                                                                        <  1:706260/300‑1 (MQ=255)                                                                                                               cTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAAc                                                                                                                                                                        <  1:706104/300‑1 (MQ=255)                                                                                                                         gCTCTGAATACCGTTGCAATTATCATTGTAGCGTTTAGGCATTTCAGGTGTTTCATCGCCCGCGCGTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTNCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGACCTGTTGACCGCATATCCGCATGCACGGGCGTGTTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGTATTAACTGTATTgcgc                                                                                                                                                              <  2:660046/300‑1 (MQ=255)                                                                                                                         gCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTNACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                              >  1:564509/1‑300 (MQ=255)                                                                                                                         gCTCTGAATACCGTGGCGACTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTgcgc                                                                                                                                                              >  1:697395/1‑300 (MQ=255)                                                                                                                           tctGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTg                                                                                                                                                            >  1:264396/1‑300 (MQ=255)                                                                                                                                   ccGTGGCGATTATCATTGTAGCGTTTAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCNCATATCCGCATGCGCGGGCGTGGTTCNCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAacac                                                                                                                                                    <  2:511902/300‑1 (MQ=255)                                                                                                                                   ccGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAacac                                                                                                                                                    <  1:97825/300‑1 (MQ=255)                                                                                                                                    cGTGGCGATTATCATTGTAGCGTTGATGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGTCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAAcacc                                                                                                                                                   <  2:312971/300‑1 (MQ=255)                                                                                                                                                 aTTGTAGTGTTTAGGCATTTCAGTCGTTTCATCGCCCGCGCGGGGAAGCCTTGCTGGATGCCGGGGCCAATCTGCTGGCCTGCGAAACCCTGCCGAACTTTTCCGAGATTGACGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGTGGGCGTGGTTCTCATCTACCCTGCGCGACAGCGAACACCTGATCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTgc                                                                                                                                      <  2:391926/300‑1 (MQ=255)                                                                                                                                                  ttGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTAAGAGATTGAGGCGTTGGCAGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGTGCTAGGCATTAAATGTATTGCGCTGGAAAACCACACCGCTGCGTTgca                                                                                                                                     >  2:237733/1‑300 (MQ=255)                                                                                                                                                            gAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGATGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTacac                                                                                                                           <  1:74491/300‑1 (MQ=255)                                                                                                                                                                    tCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAAc                                                                                                                   <  1:584028/300‑1 (MQ=255)                                                                                                                                                                      aGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACgg                                                                                                                 <  1:195994/300‑1 (MQ=255)                                                                                                                                                                        ctgcgagatgtgtataagagacaggCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGtg                                                                                                               >  1:357564/25‑300 (MQ=255)                                                                                                                                                                         cGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCAGGGGCCGATCTGCTGGCCTGCGAAACCATGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGTGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGTGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAGAACACCACGGCTGCGTTGCAGCATTTACACGGTTTAACGGtac                                                                                                              >  2:200766/1‑298 (MQ=255)                                                                                                                                                                              cATCGCCCGCGCGTGGAATCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCg                                                                                                         <  2:204487/300‑1 (MQ=255)                                                                                                                                                                              cATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCg                                                                                                         <  1:163887/300‑1 (MQ=255)                                                                                                                                                                                  gCCCGCGCGTGGAAGCCTTGCTGGATGCCGGNGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGNTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCtgg                                                                                                     >  2:527532/1‑300 (MQ=255)                                                                                                                                                                                       cgcgTGTAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGNGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGNCGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGgtggtg                                                                                                <  2:600620/300‑1 (MQ=255)                                                                                                                                                                                       cgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGgtggtg                                                                                                >  2:464042/1‑300 (MQ=255)                                                                                                                                                                                       cgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGgtggtg                                                                                                >  1:136019/1‑300 (MQ=255)                                                                                                                                                                                           tGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCCGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATc                                                                                            >  2:201086/1‑300 (MQ=255)                                                                                                                                                                                                gCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAAc                                                                                       >  2:314937/1‑300 (MQ=255)                                                                                                                                                                                                gCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAAc                                                                                       <  1:86508/300‑1 (MQ=255)                                                                                                                                                                                                 ccTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACt                                                                                      <  1:168984/300‑1 (MQ=255)                                                                                                                                                                                                           tGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCa                                                                            >  1:495958/1‑300 (MQ=255)                                                                                                                                                                                                                  gCCGATCTGCTGGCCTGCTACACCCTGCCGAATTTTTCCTAGATTGATGCGTTGGCCGAACTGTTGACCGCATATCCGCATGCGCGGGCGTGGTCCCCATTTACCCTGCGCGACAACGAACACCGGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTTGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTATACGGTTTAACGGTGCTGCCACTGGTGGTGTAGCCAAACTCGGGCGAGCATTACGac                                                                     <  2:265057/300‑2 (MQ=255)                                                                                                                                                                                                                                cTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTNTTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAAc                                                       >  1:541229/1‑300 (MQ=255)                                                                                                                                                                                                                                 tGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCNAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTNGGGCGAGCATTACGATGCCGTGAGCAAAAcc                                                      <  2:529956/300‑1 (MQ=255)                                                                                                                                                                                                                                     aaaCCCTGCCGAATTTTGCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAACATTACGATGCCGTGAGCAAAACCTGGc                                                  <  2:682077/300‑1 (MQ=255)                                                                                                                                                                                                                                       aCCCTGCCGAATTTTTCCTAGGTTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGcat                                                <  2:654263/300‑1 (MQ=255)                                                                                                                                                                                                                                                             ttGAGGCGTTGGCCGAGCTTTCTACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCTTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTTGCATCATCActttgaccccccccccc                          <  2:444553/300‑17 (MQ=255)                                                                                                                                                                                                                                                                  gCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTg                     >  1:413657/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cGAGCTGTTGACCGCATATCCGCATACGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCACTGGTGGTGTATCCGAACTCGGGCGAGCGTTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTa             <  2:11127/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCt          >  1:753538/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gCTGTTGACCACATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAAGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCAACGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTCTCCGAACTCGGGCGAGCATTACGATGCCGCGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCt          >  1:283650/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                gTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGcc       >  1:728951/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     ccGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGt  >  1:83685/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     ccGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGt  >  2:412825/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                TTGGCAAAAGCGTGGAGCTGGCGCGTAAAGCCCGTGAAGCGTATCTGGCAGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCAGGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCATCATCACGGTGAACATTGCGCGCAGCTGGCGGATTATCTGCCGCAGT  >  NC_000913/276989‑277564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑