Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,233,309	G→A	S470F (TCT→TTT)	nhaB ←	Na(+):H(+) antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,233,309	0	G	A	100.0%	254.9 / NA	74	S470F (TCT→TTT)	nhaB	Na(+):H(+) antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/31);  total (43/31)

ATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACAAACTGA  >  NC_000913/1233035‑1233587                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                       aTTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGc                                                                                                                                                                                                                                                               <  1:323534/300‑1 (MQ=255)         gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTca                                                                                                                                                                                                                                                       >  2:741739/1‑300 (MQ=255)         gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTca                                                                                                                                                                                                                                                       >  1:142792/1‑300 (MQ=255)           aaTATGCATCGAGTGCGCAGTTTAAACCACTTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCCATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGAAGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagc                                                                                                                                                                                                                                                     >  2:440337/1‑300 (MQ=255)           aaTATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTcagc                                                                                                                                                                                                                                                     >  1:446945/1‑300 (MQ=255)              atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAGAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagg                                                                                                                                                                                                                                                  >  2:476012/1‑300 (MQ=255)              atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGTGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagg                                                                                                                                                                                                                                                  <  2:228384/300‑1 (MQ=255)              atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTNTTATCAAAGCGTTGCTATCCAGCCCANTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagg                                                                                                                                                                                                                                                  >  2:508193/1‑300 (MQ=255)               tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGCGGTAAGcagga                                                                                                                                                                                                                                                 >  2:219403/1‑300 (MQ=255)               tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagga                                                                                                                                                                                                                                                 >  1:312467/1‑300 (MQ=255)               tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCACGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCAGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACNCCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcagga                                                                                                                                                                                                                                                 >  2:571899/1‑300 (MQ=255)                gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCAGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaa                                                                                                                                                                                                                                                >  1:371811/1‑300 (MQ=255)                gCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATACGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaa                                                                                                                                                                                                                                                <  1:136976/300‑1 (MQ=255)                 cATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGcaggaac                                                                                                                                                                                                                                               <  1:684538/300‑1 (MQ=255)                              gTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCt                                                                                                                                                                                                                                  >  1:682674/1‑300 (MQ=255)                                 tAAACCAATTCATTCGCTGCGCCACCAGTAGGCAGCAAAAATAGGACGCAATTGNACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGAc                                                                                                                                                                                                                               <  2:569902/300‑1 (MQ=255)                                   aaCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGCCGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCg                                                                                                                                                                                                                             >  1:127922/1‑300 (MQ=255)                                           cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCNGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGAAGCCTGACCGCTCGGCGt                                                                                                                                                                                                                     >  2:187428/1‑300 (MQ=255)                                                gCTGCGCCACCATGAGGCAGCAAAAATAGTACGCAATTGGACGATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGa                                                                                                                                                                                                                <  2:281337/300‑1 (MQ=255)                                                      ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAgg                                                                                                                                                                                                          >  1:655043/1‑300 (MQ=255)                                                         ccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCAATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGAACGTTCGGCGTAGCGACGGAGGGCa                                                                                                                                                                                                       >  1:759580/1‑300 (MQ=255)                                                             gaggAAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGAATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAtt                                                                                                                                                                                                   <  1:622073/300‑1 (MQ=255)                                                                            cgccaccattgaaccattctcgggcgacccggCAAACCGGGCATTTATATGCCCGGTACGTTGTTATCAAAGCGCTGCTATCCAGCCCATTTGCATAAACCATTCGATTACAGGGGCCCGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGCGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAACGAGGCGAATCACTGGCGCGAGTGCAGAGGTCCGCAGGAACAGGAACGCAGCATGCACGTTCGGCGTAGCGACGGAGGGCAGATTGGTACTGGTATTAAt                                                                                                                                                                                    >  2:80876/31‑300 (MQ=255)                                                                              aCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATgg                                                                                                                                                                                  <  1:752120/300‑1 (MQ=255)                                                                              aCGCAATTGGACAATTATCGAGCGAAAAGGCAAACCGGGCATTTACATGCCCGGTAAGTTGTTATCAAATCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACAGGTATGAATgg                                                                                                                                                                                  <  1:323826/300‑1 (MQ=255)                                                                                         cAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCACGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACAAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATTGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCcagc                                                                                                                                                                       <  2:270837/300‑1 (MQ=255)                                                                                                        aaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTc                                                                                                                                                        <  2:367248/300‑1 (MQ=255)                                                                                                            cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAANCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACg                                                                                                                                                    <  1:519967/300‑1 (MQ=255)                                                                                                             aaaCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGANTCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGNCGACCGCCAGCAGCTCGTATTGCTTCAACGt                                                                                                                                                   <  2:543897/300‑1 (MQ=255)                                                                                                                     cATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAGTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAATAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAgcgc                                                                                                                                           <  2:120940/300‑1 (MQ=255)                                                                                                                        ttATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCAc                                                                                                                                        >  1:71277/1‑300 (MQ=255)                                                                                                                                  ggTAAGTTGTTATCAAATCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGc                                                                                                                              <  2:406091/300‑1 (MQ=255)                                                                                                                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGTTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGATGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACATGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGc                                                                                                                              <  1:189840/300‑1 (MQ=255)                                                                                                                                  ggTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGNTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGc                                                                                                                              <  1:536243/300‑1 (MQ=255)                                                                                                                                          gttATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGAACGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCg                                                                                                                      >  1:757514/1‑300 (MQ=255)                                                                                                                                                      ttGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGTCAAGCGTAAACTCGNCGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGAAGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGNATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGatat                                                                                                          >  2:709776/1‑300 (MQ=255)                                                                                                                                                      ttGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAGGGAGGGGCAATCCACACCATGCGGCCATAAAAAAGGTAACTCAATGGCGCGATTGCAGAGGCCAGCAGAAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACAGGTCTCACTGGCGACCGCCAGCAGCTCGTCTTGCTCCACCGTGCTAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGagat                                                                                                          >  1:140160/1‑297 (MQ=255)                                                                                                                                                                   cATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCCGAGCCAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGCGGGCAGATTGGTACAGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTAAAAGTGATAGCGCCACTTTCCCTTACAGCTTTCGCTTAGTTGATATAAATTGTCTCCaa                                                                                             >  2:50343/1‑299 (MQ=255)                                                                                                                                                                   cATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCAc                                                                                             >  1:658980/1‑300 (MQ=255)                                                                                                                                                                        gCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGa                                                                                        >  2:592313/1‑300 (MQ=255)                                                                                                                                                                          aTAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGGCTACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGGATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACANGAACGCAGCCTGACCGTTCGGCGTAGNGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACg                                                                                      <  2:502931/300‑1 (MQ=255)                                                                                                                                                                               ccATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTagc                                                                                 <  1:597134/300‑3 (MQ=255)                                                                                                                                                                                               gCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGAcagc                                                                 <  1:186054/300‑1 (MQ=255)                                                                                                                                                                                                   gCGTAAGCTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGGGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACTAAGACGTTATCCGAAATGGATGACAGCAGAc                                                             <  2:147588/300‑1 (MQ=255)                                                                                                                                                                                                   gCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGAc                                                             >  1:743930/1‑300 (MQ=255)                                                                                                                                                                                                   acGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAACCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGAc                                                             >  2:211376/2‑300 (MQ=255)                                                                                                                                                                                                    cGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGCACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGAcc                                                            >  2:277503/1‑300 (MQ=255)                                                                                                                                                                                                              aCGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAATGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACTGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCAGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAata                                                  >  2:328358/1‑300 (MQ=255)                                                                                                                                                                                                              aCGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAata                                                  >  1:666351/1‑300 (MQ=255)                                                                                                                                                                                                                   gagCAAGCCGACGAGTGTCAGGACGAGGGTGNAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGNTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGaa                                             >  2:527104/1‑300 (MQ=255)                                                                                                                                                                                                                    agCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGANCGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                                            <  1:514614/300‑1 (MQ=255)                                                                                                                                                                                                                    agCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACTTGATAGCGCCACTTTCCATTGCCGCTTTCGCCTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                                            <  2:442161/300‑1 (MQ=255)                                                                                                                                                                                                                    agCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                                            <  2:71038/300‑1 (MQ=255)                                                                                                                                                                                                                                 gtgtCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAg                               >  1:623221/1‑300 (MQ=255)                                                                                                                                                                                                                                  tgtCAGGACTAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGNCGAATCAATTGCGCGAGTGCAGAGTTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTACCACTTTCGCTGCGTTGAGATAAATCGTCCCCACGAAGACGTTATCCGAAATGGACGACAGCAGACCGGTGAAAAGATAAAACAGCGACAGCTGAGc                              <  2:558960/300‑1 (MQ=255)                                                                                                                                                                                                                                   gtCAGGACGCGGGTGTAAGGCAGGGCCCTCTACACCATGCGGCCATAAAAGAGGCGCATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGTAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGCCCTGGATGACAGCAGACCATTGAAAATATAGAACAGCGACAGCTGAgaa                             >  2:210818/1‑298 (MQ=255)                                                                                                                                                                                                                                    tCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCAt                            <  1:446595/300‑1 (MQ=255)                                                                                                                                                                                                                                        gACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATNAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGCTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCNGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTc                        <  2:705486/300‑1 (MQ=255)                                                                                                                                                                                                                                            aGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGAt                    >  1:66939/1‑300 (MQ=255)                                                                                                                                                                                                                                             gggTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATNGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATg                   >  1:535557/1‑300 (MQ=255)                                                                                                                                                                                                                                              ggTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAACGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACACAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAACTCGTATTGCTTCAACGTGATAGCGCAACTTTCCATTGCCGATTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGACATGGATGACAGCAGACTGTTGAAAATATAGAACAGCGACAGCCGAGCCTATTCCgctac                  >  2:311787/1‑296 (MQ=255)                                                                                                                                                                                                                                               gtgtAAGGCCGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGCACGTTCGGCGTAGCGACGGAAGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAACTCGTATTGCTTCAGCGTGATAGCGCCAATTTACATTGCCGCTTTAGCTCCGTTGATATAAATCGTCCCCACGAAAACGTTATCCGAAATAGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGca                 >  2:33256/1‑299 (MQ=255)                                                                                                                                                                                                                                               gtgtAAGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGcc                 >  1:277597/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aGGCAGGGCCATCCACACCATGCTGCCATAAAAGAGGCGAATCAATTGCGCGAGNGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCC‑AAATGGATGACNGCAGACCGTTGAAAATATAGAACAGCGACAGcctgtctcttagacacatcccgcag           <  2:700701/300‑25 (MQ=255)                                                                                                                                                                                                                                                    aGGCAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGNAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCAGAAATGGATGACANCAGACCGTTGAAAATATCGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCaa            >  2:706529/1‑300 (MQ=255)                                                                                                                                                                                                                                                       cAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAGTGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAacac         <  2:110575/300‑1 (MQ=255)                                                                                                                                                                                                                                                       cAGGGCCATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAacac         <  2:223135/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gCCATCCACACCCTGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCCCGAAGACGCTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAAAGCGAAAGCTGAGCATGTTCCGATGCATGCAACACAAAc     >  1:74529/1‑300 (MQ=255)                                                                                                                                                                                                                                                            ccATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACAAACt    <  1:220747/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCCGCAGGAACAAGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAAACTGGTACCGGTCTTAATGGCGACCGCCAGCAGCTCGTATTGCTTCCACGTGATAGCGCCACTTTCAATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCAACGAAGACGCTATCCGAAATGGATGGCAGCCGACCGTTGAAAATATAGAAAAGCGAAAGCTGAGAATATTTCGATGCCTGCAacaacaactt   >  2:293184/1‑293 (MQ=255)                                                                                                                                                                                                                                                             cATCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGACTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGCTATAAATCGTCCCCCCGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACAAACTg   >  2:724842/1‑300 (MQ=255)                                                                                                                                                                                                                                                              aTCCACACCATGCGGCCATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAACTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGCACAGCGACCGCTGAGCATGTTCCGATGCCTGCACCACAAACTGa  >  2:582891/1‑300 (MQ=255)                                                                                                                                                                                                                                                              aTCCACACCATGCGGCCATAAAAGAGGCGAANCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGNGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACAAACTGa  >  2:540234/1‑300 (MQ=255)                                                                                                                                                                                                                                                              aTCCACACCATGCGGCAATAAAAGAGGCGAATCAATGGCGCGAGTGCAGAGGCCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCAACTTTCCATTGCCGCTTTCGCTTCGTTGATATCAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACCAACTGa  >  1:300803/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                       ATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGCCTGCAACACAAACTGA  >  NC_000913/1233035‑1233587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑