Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,193,645	G→A	D43D (GAC→GAT)	mnmA ←	tRNA‑specific 2‑thiouridylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,193,645	0	G	A	100.0%	308.7 / NA	86	D43D (GAC→GAT)	mnmA	tRNA‑specific 2‑thiouridylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/42);  total (44/42)

CAGGCGGCTCTTGCCATCGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCGTCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGAT  >  NC_000913/1193351‑1193939                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       cagGCGGCTCTTGCCATCGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCTGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCAtcttc                                                                                                                                                                                                                                                                                                   <  1:33317/300‑1 (MQ=255)      ggcTCTTGCCATCGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTccc                                                                                                                                                                                                                                                                                              <  1:490674/300‑1 (MQ=255)       gcTCTTGCCATCGACATTGGCCCGACGTACGTAATGACTGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAACATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCa                                                                                                                                                                                                                                                                                             >  2:18005/1‑300 (MQ=255)       gcTCTTGCCATCGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCa                                                                                                                                                                                                                                                                                             >  2:466534/1‑300 (MQ=255)             gCCATCGACATCGGCACGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTAACCGTCATCTTCTTCCCAGctctt                                                                                                                                                                                                                                                                                       >  2:254191/1‑300 (MQ=255)                  cGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCAAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATaa                                                                                                                                                                                                                                                                                  >  2:348500/1‑300 (MQ=255)                    aCATCGGCCCGACGTACGTAATGACCGTTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAAc                                                                                                                                                                                                                                                                                <  1:171656/300‑1 (MQ=255)                    aCATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAAc                                                                                                                                                                                                                                                                                <  1:128917/300‑1 (MQ=255)                       tCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAgg                                                                                                                                                                                                                                                                             <  1:764860/300‑1 (MQ=255)                       tCGGCCCGACGTACGTAAGGACCGGTAGCGATATAATCGGCACCGAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTGGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCGTCCCAGTTCTTCATAAACAgg                                                                                                                                                                                                                                                                             <  1:765121/300‑1 (MQ=255)                              gACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCAAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGa                                                                                                                                                                                                                                                                      >  1:289822/1‑300 (MQ=255)                               acgtacgtAATGACCGTTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTGCTTCATAAACAGGCCTTCGAc                                                                                                                                                                                                                                                                     <  2:374270/300‑1 (MQ=255)                                cgtacgtaATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGAcc                                                                                                                                                                                                                                                                    <  2:64556/300‑1 (MQ=255)                                cgtacgtaATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATNCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTNTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGAcc                                                                                                                                                                                                                                                                    <  2:710915/300‑1 (MQ=255)                                cgtacgnaATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATNCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGAcc                                                                                                                                                                                                                                                                    <  2:629032/300‑1 (MQ=255)                                 gtacgtaATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCt                                                                                                                                                                                                                                                                   >  1:254676/1‑300 (MQ=255)                                         tGACCGTTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATccc                                                                                                                                                                                                                                                           <  2:485667/300‑1 (MQ=255)                                            ccGGTAGCGATATAATCTGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCtgt                                                                                                                                                                                                                                                        <  1:312144/300‑1 (MQ=255)                                                  gCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGAACTGATATCCCTGTTGTTGc                                                                                                                                                                                                                                                  >  1:13145/1‑300 (MQ=255)                                                     atatAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAAc                                                                                                                                                                                                                                               >  1:219191/1‑300 (MQ=255)                                                     atatAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCAAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAAc                                                                                                                                                                                                                                               >  1:478040/1‑300 (MQ=255)                                                             ggCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGc                                                                                                                                                                                                                                       <  1:292605/300‑1 (MQ=255)                                                                 ccTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGaa                                                                                                                                                                                                                                   >  1:246117/1‑300 (MQ=255)                                                                  cTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCANACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGNTATCCCTGTTGTTGCAACAGCCAGGCAGaaa                                                                                                                                                                                                                                  <  2:543062/300‑1 (MQ=255)                                                                                  aaaTTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGNACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACAcc                                                                                                                                                                                                                  <  1:533425/300‑1 (MQ=255)                                                                                         aGGAAGGCTTTAAATTTGATCTCTTTTTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGAc                                                                                                                                                                                                           <  2:457795/300‑1 (MQ=255)                                                                                              ggCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGcc                                                                                                                                                                                                      <  1:756314/300‑1 (MQ=255)                                                                                                                 ttgttgCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTTCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCg                                                                                                                                                                                   >  1:720711/1‑300 (MQ=255)                                                                                                                           gAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATANACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGACGGACCTGCCGACGATTACTTTTTTTGCGGTTTCAGACa                                                                                                                                                                         >  2:671656/1‑300 (MQ=255)                                                                                                                           gAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACa                                                                                                                                                                         >  1:496048/1‑300 (MQ=255)                                                                                                                           gAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTCTTTTGCGATTTCAGACa                                                                                                                                                                         >  2:91004/1‑300 (MQ=255)                                                                                                                                tCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTGAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCTTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGa                                                                                                                                                                    <  2:587411/300‑1 (MQ=255)                                                                                                                                 ccGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGAt                                                                                                                                                                   <  1:183692/300‑1 (MQ=255)                                                                                                                                    gATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGAtcac                                                                                                                                                                >  1:290426/1‑300 (MQ=255)                                                                                                                                           cGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCNGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGAc                                                                                                                                                         <  1:521064/300‑1 (MQ=255)                                                                                                                                           cGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCNGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCCCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGAc                                                                                                                                                         <  1:521086/300‑1 (MQ=255)                                                                                                                                                gACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGa                                                                                                                                                    <  2:470488/300‑1 (MQ=255)                                                                                                                                                  ccGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAAc                                                                                                                                                  <  1:608043/300‑1 (MQ=255)                                                                                                                                                    ggCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACtt                                                                                                                                                <  1:9492/300‑1 (MQ=255)                                                                                                                                                     gCTTTCTATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCCGCCAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCNCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGAAGATTCCTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTc                                                                                                                                               >  1:518259/1‑300 (MQ=255)                                                                                                                                                       tttATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCaa                                                                                                                                             <  1:672998/300‑1 (MQ=255)                                                                                                                                                                   aaGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTc                                                                                                                                 <  2:734193/300‑1 (MQ=255)                                                                                                                                                                      gAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCAAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTAt                                                                                                                              >  2:325524/1‑300 (MQ=255)                                                                                                                                                                        aCAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCa                                                                                                                            <  2:744360/300‑1 (MQ=255)                                                                                                                                                                                aaGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGTGTATTCTATCACGCAGCCt                                                                                                                    <  1:156594/300‑1 (MQ=255)                                                                                                                                                                                aaGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCt                                                                                                                    <  1:153831/300‑1 (MQ=255)                                                                                                                                                                                    cGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGANGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTa                                                                                                                >  1:507690/1‑300 (MQ=255)                                                                                                                                                                                     gTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAg                                                                                                               <  1:239851/300‑1 (MQ=255)                                                                                                                                                                                      ttGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTAGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAGTCGACACCGCCGGACATGCCGACGATTAATTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTCTCACGTAGCCTTTTAGa                                                                                                              >  2:365625/1‑300 (MQ=255)                                                                                                                                                                                        gTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCACGGCGGCGTATTCTATCAAGCAGCCTTTTAGATg                                                                                                            >  1:624408/1‑300 (MQ=255)                                                                                                                                                                                        gTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATg                                                                                                            <  1:499452/300‑1 (MQ=255)                                                                                                                                                                                        gTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATg                                                                                                            >  2:401393/1‑300 (MQ=255)                                                                                                                                                                                              gTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACAccc                                                                                                      <  1:318301/300‑1 (MQ=255)                                                                                                                                                                                              gTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCAACGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACAccc                                                                                                      >  1:100637/1‑300 (MQ=255)                                                                                                                                                                                                           aaGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGcc                                                                                         >  1:277380/1‑300 (MQ=255)                                                                                                                                                                                                            aGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCa                                                                                        >  1:691307/1‑300 (MQ=255)                                                                                                                                                                                                                 aCGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTaa                                                                                   >  1:349569/1‑300 (MQ=255)                                                                                                                                                                                                                      gtgCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCg                                                                              >  1:623924/1‑300 (MQ=255)                                                                                                                                                                                                                           gTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAACATCGCCAat                                                                         >  2:439024/1‑300 (MQ=255)                                                                                                                                                                                                                                aTGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGAACTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATNCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCAtct                                                                    >  2:187294/1‑300 (MQ=255)                                                                                                                                                                                                                                           gTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATaa                                                         >  1:417483/1‑300 (MQ=255)                                                                                                                                                                                                                                             cGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACg                                                       <  1:693950/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGt                                                      <  1:82413/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGt                                                      <  2:468373/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCAGACAGCCGGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCGCACCGTCATCTTCTTCCCAGTTCTGCATAAACAGGCCTGCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTGTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCCATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGt                                                      <  2:251602/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCNGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAANCGCCAATCATCTCCAGCGGATAACGTTGccc                                                 <  2:526032/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGccc                                                 <  2:77045/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGc                                               >  2:474542/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGc                                               >  1:185384/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCAAGTTAAAATCGCAAATCATCTCCAGCGGCTAACGTTGCCCGc                                               >  1:185936/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATCCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGc                                               >  1:156940/1‑300 (MQ=255)                                                                                                                                                                                                                                                            cATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATa                                        <  1:355566/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATaacaa                                    >  1:133272/1‑300 (MQ=255)                                                                                                                                                                                                                                                                aGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCAAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATaacaa                                    >  2:733586/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gATCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAAAGTTGCCCGCTTTGATAACAACGAAt                               >  2:435621/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       tCCGCTGCCGCTGTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATAc                             <  1:476553/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ctgccgctgTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTc                         <  1:476062/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gctgTGCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCTACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGcca                    >  1:644451/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  tgtgCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGccacc                  <  2:161341/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gtgCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGccacca                 >  1:3888/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   gtgCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCAAGCGGATAACGTTGCCCGCTTTGATACCAACGAATACTTTCCGccacca                 >  2:414684/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gCAATATTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCNCGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAgc               >  1:540589/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        atatTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGNAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAgcggc            >  1:539414/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          atTCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCAGCCAACAGCGGcga          >  2:734568/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tCCTCACCGTCATCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAANGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGcgaac        >  2:172922/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  ccGTCATCTTCTTACCAGTTCTTCATAAACAGGCCTTCGAACTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCAGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATCGAATCACTCACGACATTGAACTTAAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCGTCTCAAGCGGATAACGTTGCCCGCTTTGCTAACGACGAATCCTTTCCGCCACCAGCGGcgaacgaaaat  >  2:84500/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       CAGGCGGCTCTTGCCATCGACATCGGCCCGACGTACGTAATGACCGGTAGCGATATAATCGGCACCTAAATCTTCGGCGGCAAATTCGAGGAAGGCTTTAAATTTGATCTCTTTGTTGCACAGAATATCCGGATTCGGCGTGCGACCGGCTTTATATTCGGCAAGGAACAGTTCGAAGACGTTGTCCCAGTACTCGGCAGCAAAGTTAACGGTGTGCAGTTCAATGCCGAGCTTGTCGCAGACAGCCTGGGCATCAGCCAGATCCGCTGCCGCTGTGCAATATTCCTCACCGTCGTCTTCTTCCCAGTTCTTCATAAACAGGCCTTCGACCTGATATCCCTGTTGTTGCAACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGAT  >  NC_000913/1193351‑1193939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑