Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,064	G→A	R213H (CGT→CAT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,064	0	G	A	100.0%	217.6 / NA	61	R213H (CGT→CAT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/34);  total (27/34)

GCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATC  >  NC_000913/1175768‑1176356                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      gCTCGATATCAACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGACGGGGCGTATTCCCAGCCAGCGCCTGTTCAATTGGATTGGCACTTTCGCCGCCAACAGTGAAGTAGATGGCTATGAAATGCTGGTGAATATTAAGGATGCCTCGCGTCTGATGCATTa                                                                                                                                                                                                                                                                                                    >  1:698364/1‑300 (MQ=255)    cGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATcc                                                                                                                                                                                                                                                                                                 >  1:20574/1‑300 (MQ=255)                     aaaaaGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCGAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGAATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCg                                                                                                                                                                                                                                                                                <  1:750473/300‑1 (MQ=255)                              cACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATGATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCTGCAGGCAATATTACCGGCTGGCGtt                                                                                                                                                                                                                                                                       >  2:480233/1‑300 (MQ=255)                                    cacCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGc                                                                                                                                                                                                                                                                 >  2:143482/1‑300 (MQ=255)                                    cacCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGc                                                                                                                                                                                                                                                                 <  1:144285/300‑1 (MQ=255)                                     acCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCt                                                                                                                                                                                                                                                                <  2:493545/300‑1 (MQ=255)                                         tATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGAt                                                                                                                                                                                                                                                            >  1:308679/1‑300 (MQ=255)                                                   aTGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGa                                                                                                                                                                                                                                                  >  1:392280/1‑300 (MQ=255)                                                       gAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAATAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGt                                                                                                                                                                                                                                              <  2:381888/300‑1 (MQ=255)                                                       gAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGTGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATTAAATGCTGGTGAATATTGAGGATGCCTCGCGTCCGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGt                                                                                                                                                                                                                                              <  2:381478/300‑1 (MQ=255)                                                       gAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTTTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGt                                                                                                                                                                                                                                              <  2:741514/300‑1 (MQ=255)                                                                   ccTCGAGCCGTGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAg                                                                                                                                                                                                                                  <  1:42262/300‑1 (MQ=255)                                                                             gggAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACCGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATGTTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTACGTCAGCaaaaa                                                                                                                                                                                                                        >  1:762956/1‑300 (MQ=255)                                                                                  atatAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATNTTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGcc                                                                                                                                                                                                                   >  1:550001/1‑300 (MQ=255)                                                                                  atatAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCATGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTAGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCNTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAANACTGcc                                                                                                                                                                                                                   >  2:618765/1‑300 (MQ=255)                                                                                       aTGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAg                                                                                                                                                                                                              >  1:168479/1‑300 (MQ=255)                                                                                                   gCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGc                                                                                                                                                                                                  >  2:426833/1‑300 (MQ=255)                                                                                                        caacTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGAc                                                                                                                                                                                             <  2:491863/300‑1 (MQ=255)                                                                                                        caacTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATACTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTTGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAATGCAGCAAATGGCAGGAc                                                                                                                                                                                             <  2:275266/300‑1 (MQ=255)                                                                                                             tGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGTAAATGGCAGGACTGGCg                                                                                                                                                                                        <  1:485494/300‑1 (MQ=255)                                                                                                             tGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCg                                                                                                                                                                                        <  1:270342/300‑1 (MQ=255)                                                                                                             tGCCTCACAGCTAGGCGGTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCg                                                                                                                                                                                        <  2:87429/300‑1 (MQ=255)                                                                                                                          ggCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGc                                                                                                                                                                           >  2:575704/1‑300 (MQ=255)                                                                                                                          ggCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATNACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGc                                                                                                                                                                           >  2:668547/1‑300 (MQ=255)                                                                                                                           gCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCANGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAANTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCg                                                                                                                                                                          <  2:695659/300‑1 (MQ=255)                                                                                                                                        tgagcAAATCCGCTTGATTGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                             <  2:678163/296‑1 (MQ=255)                                                                                                                                        tGATCAAATCCTCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                             <  1:285695/300‑1 (MQ=255)                                                                                                                                        tGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCTCCAACAGTGAAGTCGNTGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCNATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                             <  2:607517/300‑1 (MQ=255)                                                                                                                                        tGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                             <  1:672047/300‑1 (MQ=255)                                                                                                                                        tGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACGGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGc                                                                                                                                                             <  1:24321/300‑1 (MQ=255)                                                                                                                                         gATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGcc                                                                                                                                                            >  1:423495/1‑300 (MQ=255)                                                                                                                                         gATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGcc                                                                                                                                                            >  2:471627/1‑300 (MQ=255)                                                                                                                                                             aCCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAatat                                                                                                                                        >  1:620523/1‑300 (MQ=255)                                                                                                                                                                          cagTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACATTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActg                                                                                                                           <  1:257660/300‑1 (MQ=255)                                                                                                                                                                           agTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTTTTCAATGTGATTGGCACTTTCGCCGCCAACATTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgc                                                                                                                          <  2:446140/300‑1 (MQ=255)                                                                                                                                                                           agTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgc                                                                                                                          <  1:63201/300‑1 (MQ=255)                                                                                                                                                                            gTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTActgct                                                                                                                         >  1:679016/1‑300 (MQ=255)                                                                                                                                                                                       tGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTg                                                                                                              <  2:441565/300‑1 (MQ=255)                                                                                                                                                                                       tGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTg                                                                                                              <  1:345222/300‑1 (MQ=255)                                                                                                                                                                                            cGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGcc                                                                                                         <  2:341278/300‑1 (MQ=255)                                                                                                                                                                                                ttCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTg                                                                                                     >  1:61335/1‑300 (MQ=255)                                                                                                                                                                                                           cgcCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAAc                                                                                          >  1:263819/1‑300 (MQ=255)                                                                                                                                                                                                             catGTTCAATTTTATGGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAGATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGTCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACat                                                                                        <  2:156175/298‑1 (MQ=255)                                                                                                                                                                                                                              gcgcTTTCGCCGCCAACAGTTAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCGGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGc                                                                      <  2:357902/297‑1 (MQ=255)                                                                                                                                                                                                                              gCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGc                                                                      <  1:695210/300‑1 (MQ=255)                                                                                                                                                                                                                                    tCGCCGCCAACAGTGAATGCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATgg                                                                <  1:436663/300‑1 (MQ=255)                                                                                                                                                                                                                                          ccAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATgg                                                          <  1:472370/300‑1 (MQ=255)                                                                                                                                                                                                                                                gTGAAGTCGATGGCTATGAAATGCTGGTGAATAGTGAGGAGGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGGGGCTGGATGAGCCGCTGAAANTCGACTCAGTAAGTCAGCAAAAACTGCCTGAATGCAGCAAATGGCAGGACTGGCGTGATCGNAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTGAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGc                                                    <  2:608535/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAg                                        >  1:22139/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAg                                        >  2:748188/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aTGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGCGCCTGATTGTCGCCGTTGCGGCGTTTACCATTATTACCT‑ACATAGGGCTGATGGTAATGGAGAAGCAGGGCGAAATAGCGa                                     >  2:163560/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ggTGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAAc                           >  2:116130/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tGAATATTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGc                         <  1:450550/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              atatTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTACCATTATTACCTCAA‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAg                      >  2:398015/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              atatTGAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTCGCGATCCTGCAAACGCAAg                      >  2:408826/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   gAGGATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCATGATTGTCGCCGTTGCGGCGTTTAACATTATTAACTAAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTa                 >  2:35061/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     ggATGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAAc               <  1:35151/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aTGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTc             <  2:167458/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aTGCCTCGCGTCTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTc             <  1:600934/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  cTGATGCATTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGAATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGTGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTGATGGAGAAGCAGGGCGAAGTAGCGATCCTGCACACGCAAGACTTAACTCAGCGACAAATc  >  2:338289/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                      GCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCAC‑TAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATC  >  NC_000913/1175768‑1176356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑