Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,402	G→A	E199E (GAG→GAA)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,402	0	G	A	100.0%	198.2 / NA	56	E199E (GAG→GAA)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

ATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGT  >  NC_000913/1127110‑1127695                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      aTGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATNGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCAGAACACCAtt                                                                                                                                                                                                                                                                                                >  2:565199/1‑300 (MQ=255)   gCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGATAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCAtttt                                                                                                                                                                                                                                                                                              >  1:679209/1‑300 (MQ=255)                                     aaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGc                                                                                                                                                                                                                                                            <  1:423318/300‑1 (MQ=255)                                     aaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGc                                                                                                                                                                                                                                                            <  2:419229/300‑1 (MQ=255)                                     aaCTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATGTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGc                                                                                                                                                                                                                                                            <  1:408452/300‑1 (MQ=255)                                                                 tcgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATCGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAAc                                                                                                                                                                                                                                <  2:736488/300‑1 (MQ=255)                                                                 tcgtTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAAc                                                                                                                                                                                                                                <  2:112321/300‑1 (MQ=255)                                                                        gCACCACTCTACGGTGAGGTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCGGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGAGGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAg                                                                                                                                                                                                                         <  1:339988/300‑1 (MQ=255)                                                                           ccacTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGcc                                                                                                                                                                                                                      <  1:459787/300‑1 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATANACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGANCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  2:698185/1‑300 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAGACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  2:688003/1‑300 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGNTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  2:672187/1‑300 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGTATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGcagt                                                                                                                                                                                                                    >  2:439392/1‑297 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGcagt                                                                                                                                                                                                                    >  1:195366/1‑297 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  1:713501/1‑300 (MQ=255)                                                                             acTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTATGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  1:603204/1‑300 (MQ=255)                                                                             acTCTAAGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCAGGAAGTCAGCGTGAAACCGTGCAGGCCGt                                                                                                                                                                                                                    >  2:148746/1‑300 (MQ=255)                                                                              ctctACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGtg                                                                                                                                                                                                                   >  1:769620/1‑300 (MQ=255)                                                                                                          ccACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACgg                                                                                                                                                                                       <  1:202724/300‑1 (MQ=255)                                                                                                           cACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGa                                                                                                                                                                                      <  1:341104/300‑1 (MQ=255)                                                                                                            aCCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCANAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAANACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGat                                                                                                                                                                                     >  2:603436/1‑300 (MQ=255)                                                                                                            aCCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGat                                                                                                                                                                                     >  1:465437/1‑300 (MQ=255)                                                                                                                      cgtgggctcggagatgtgtataagagacaggCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAAt                                                                                                                                                                           <  1:358314/270‑1 (MQ=255)                                                                                                                               tGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCNGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACCGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTgagg                                                                                                                                                                  <  2:558617/300‑1 (MQ=255)                                                                                                                                 gATAAACATCTTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGTTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTgaggag                                                                                                                                                                <  2:357493/300‑1 (MQ=255)                                                                                                                                            tAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGACGTGCAGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAggg                                                                                                                                                     >  2:134581/1‑300 (MQ=255)                                                                                                                                                   aGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTg                                                                                                                                              <  2:679888/300‑1 (MQ=255)                                                                                                                                                    gCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAAtctgcctcttatacacatctcgcaggggata                                                                                                                                             >  2:354156/1‑270 (MQ=255)                                                                                                                                                    gCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGc                                                                                                                                             >  2:266278/1‑300 (MQ=255)                                                                                                                                                          ggCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTTGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAAc                                                                                                                                       <  2:2301/300‑1 (MQ=255)                                                                                                                                                               cacGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGCAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGAtt                                                                                                                                  >  2:416446/1‑300 (MQ=255)                                                                                                                                                                           ttCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCag                                                                                                                      <  2:118818/300‑1 (MQ=255)                                                                                                                                                                                   gCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGAGGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTNTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCCCCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCt                                                                                                              <  2:662036/300‑1 (MQ=255)                                                                                                                                                                                        atgatTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGc                                                                                                         >  1:448089/1‑300 (MQ=255)                                                                                                                                                                                               tCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTgg                                                                                                  >  2:486764/1‑300 (MQ=255)                                                                                                                                                                                                      gtggtgGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTc                                                                                           >  2:681087/1‑300 (MQ=255)                                                                                                                                                                                                        ggtggATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCgg                                                                                         >  2:304560/1‑300 (MQ=255)                                                                                                                                                                                                           ggATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCtg                                                                                      >  1:506425/1‑300 (MQ=255)                                                                                                                                                                                                                                             tctctGGATGGCGGTACGCTACTGACTCACGACGCTGGCGCAATGCTGTTTGCCGAACACCATTCTTCAGCTGGTCCTTTGCCGATCACCACCTGTCTGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCCTCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGTATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCGCTCAATTGAATGTGTGCaaagacag                                                    >  2:288014/1‑295 (MQ=255)                                                                                                                                                                                                                                                  ggATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCNGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGt                                               >  1:562170/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aTGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTc                                             <  1:748107/300‑1 (MQ=255)                                                                                                                                                                                                                                                         ggTACGCTACTGACTAACGACGCTGTCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCACTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGTTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaa                                        <  2:31781/300‑1 (MQ=255)                                                                                                                                                                                                                                                         ggTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGGCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCaa                                        <  2:644561/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gCTACTGACTAACGACGCTGGCGAAATGCTGTCTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGAATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTCGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAACAACGTGGGTTTGTCGGCTGTGCGCGGCACTTAATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccgc                                   >  2:27365/1‑300 (MQ=255)                                                                                                                                                                                                                                                               cTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGNTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCANAccgcc                                  >  2:621232/1‑300 (MQ=255)                                                                                                                                                                                                                                                               cTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAccacc                                  >  2:306661/1‑297 (MQ=255)                                                                                                                                                                                                                                                                      cTAACGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAAc                           >  1:474406/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        aaCGACGCTGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTATTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAg                         <  2:748680/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ttgctaaaTGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAGACCGCCGGCGAACAGGCCGTGCt                 <  2:57246/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                tGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTTAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGTGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt                 <  1:251710/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                tGGCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCt                 <  1:656285/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gCGAAATGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTgg               <  1:615926/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aTGCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTAAGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTAGGCTGTGCGCGGCACCTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGTTGGCGCaa          >  2:245178/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gCTGTTTGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGNTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACg        <  1:524503/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGt  <  1:704394/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tGCCGAACACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGt  <  2:14298/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      ATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGTTTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCCTCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACGTTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCCTCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGT  >  NC_000913/1127110‑1127695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑