Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,070,238	G→A	V39V (GTC→GTT)	rutF ←	FMN reductase RutF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,070,238	0	G	A	100.0%	178.0 / NA	51	V39V (GTC→GTT)	rutF	FMN reductase RutF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/17);  total (34/17)

GCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTA  >  NC_000913/1069948‑1070530                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                     gcgcAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTg                                                                                                                                                                                                                                                                                             >  1:589960/1‑300 (MQ=255)      aaaCAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCACGTCTGCCAGGGGGCGGCGGCAAAGCGGTGTTCCATGGGTGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGt                                                                                                                                                                                                                                                                                        >  2:637142/1‑300 (MQ=255)                             cGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGTTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCNTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGNGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGAc                                                                                                                                                                                                                                                                 <  2:607364/300‑1 (MQ=255)                                         ggCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGa                                                                                                                                                                                                                                                     >  1:349700/1‑300 (MQ=255)                                               tACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCNAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTGACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAatat                                                                                                                                                                                                                                               <  2:663975/300‑1 (MQ=255)                                                      gTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGAccgc                                                                                                                                                                                                                                        <  2:734215/300‑1 (MQ=255)                                                             gAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGNCTCCTGCCCGGCGCTAAGTGGATTTACACACAGCGTTCGAGTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCATTAATGTGGGCGGCGTATCGGTCACACTGCAAACTGCGCTGGCGGTGAACTCGGCGCGCCCGGCTGGACCGTCCGTGGTGAGAATAGGGACCGCCGCGccc                                                                                                                                                                                                                                 <  1:524798/300‑1 (MQ=255)                                                                      gCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGAc                                                                                                                                                                                                                        >  1:581817/1‑300 (MQ=255)                                                                      gCCTCTNCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCANAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATCGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGAc                                                                                                                                                                                                                        <  2:631901/300‑1 (MQ=255)                                                                                             ggTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACATAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTttgttg                                                                                                                                                                                                 >  2:286357/1‑300 (MQ=255)                                                                                             ggTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTttgttg                                                                                                                                                                                                 >  1:570379/1‑300 (MQ=255)                                                                                                          tGCCAGTGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGANTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTc                                                                                                                                                                                    <  2:667513/300‑1 (MQ=255)                                                                                                          tGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAGAAGTTTTGAAAGCTGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGANTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTc                                                                                                                                                                                    <  2:667522/300‑1 (MQ=255)                                                                                                          tGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTc                                                                                                                                                                                    <  1:177601/300‑1 (MQ=255)                                                                                                              aGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAg                                                                                                                                                                                <  1:599517/300‑1 (MQ=255)                                                                                                                                                   gccgccAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTCCAACAGCCCGCAGGCTTCTTCAAAgg                                                                                                                                           >  1:186259/1‑300 (MQ=255)                                                                                                                                                                                     gtgcTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCAGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGTGCGCATAAAGCTTgttgct                                                                                                         >  1:105840/3‑300 (MQ=255)                                                                                                                                                                                     gcgcTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGTGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACTGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTAATCGACACTGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAAACGTGGCAGGCGCGCATAAAGCTtgcttct                                                                                                         >  1:106157/1‑297 (MQ=255)                                                                                                                                                                                     gcgcTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGAGAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTgctgct                                                                                                         >  2:459172/1‑300 (MQ=255)                                                                                                                                                                                     gcgcTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTgctgct                                                                                                         >  1:463454/1‑300 (MQ=255)                                                                                                                                                                                          aaGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTCAAACCGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGc                                                                                                    >  2:469681/1‑300 (MQ=255)                                                                                                                                                                                             tgtATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCAt                                                                                                 <  1:331406/300‑1 (MQ=255)                                                                                                                                                                                              gtATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                                >  1:447628/1‑300 (MQ=255)                                                                                                                                                                                              gtATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                                >  2:407027/1‑300 (MQ=255)                                                                                                                                                                                              gtATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTAACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCCTAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCACAGGACCGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                                >  2:647091/1‑300 (MQ=255)                                                                                                                                                                                                 tttACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCATTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGcc                                                                                             >  1:643934/1‑300 (MQ=255)                                                                                                                                                                                                           gCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATc                                                                                   <  1:32098/300‑1 (MQ=255)                                                                                                                                                                                                                          tGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTc                                                                    <  1:51612/300‑1 (MQ=255)                                                                                                                                                                                                                            aaTGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAg                                                                  <  1:726894/300‑1 (MQ=255)                                                                                                                                                                                                                                       gacggacgCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGACAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACATCCCGCAGGCTTCTTCACAGGACAGCCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGAAGATTGCTCTTCAGcatgttgcacg                                                       >  2:206195/1‑290 (MQ=255)                                                                                                                                                                                                                                       gacggacgCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGNTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGNTGCCCg                                                       >  2:633023/1‑300 (MQ=255)                                                                                                                                                                                                                                               ccccACGATTCAGGCACACCAGTAATGTGGGCGGGGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATNCAGGACATCGCATCGCGAAAAGTTTGNTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAg                                               >  2:512934/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTTGCCGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgtcg                                         >  2:12982/1‑300 (MQ=255)                                                                                                                                                                                                                                                      aTTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgtcgt                                        >  1:764567/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ggCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACg                                   <  1:431270/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cacaCCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGTTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACgtg                                 <  1:286186/300‑1 (MQ=255)                                                                                                                                                                                                                                                               cacCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGtt                               >  1:82406/1‑300 (MQ=255)                                                                                                                                                                                                                                                               cacCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGtt                               >  2:375267/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGa                             >  2:163271/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGa                             >  1:436723/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTAAGCGTGCTGCACGTAAAAAAGGCGTCGTCCACGTGTTGa                             >  1:393290/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 ccAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTAAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGa                             >  2:384591/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATCGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGAc                            <  2:132281/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gTAATGTGAGCGGCATATCGGCCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACTGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACgg                          >  1:670052/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      aaTGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGT‑ATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCa                       >  1:717226/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                gggTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCACGAc              >  2:739229/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                gcgTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCNACAATGTTCATAGTTTGCTCCTTACANCAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGAc              >  2:518812/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTATCGGTCACACTGCAAACGGCGCTGGCTGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGTTGATAATATTGACCGCCGCACCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt            <  2:240522/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt            >  1:379955/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCCAAGCCCGACATCggg        >  2:722979/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            cacTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGAACGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCATCACAAAGGCGTCGTCCACGTGTTGACGGTCACAGCCCGACATCGGGCCGGTa  >  2:58686/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                     GCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTA  >  NC_000913/1069948‑1070530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑