Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,070,014	A→G	L114P (CTG→CCG)	rutF ←	FMN reductase RutF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,070,014	0	A	G	97.8%	157.4 / NA	45	L114P (CTG→CCG)	rutF	FMN reductase RutF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/23);  minor base T (0/1);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCC  >  NC_000913/1069720‑1070307                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      cgcgTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGcc                                                                                                                                                                                                                                                                                                  >  1:464934/1‑300 (MQ=255)            cGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTg                                                                                                                                                                                                                                                                                       <  1:753679/300‑1 (MQ=255)             gCCCATTACTGCCGTCTGAGCAAACGGCCATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                                                                                                                                                                      >  2:283718/1‑300 (MQ=255)             gCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGCAGGGTGCGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCCGTCAAACGAAACCGGAGCCTCTTCCAGtcgc                                                                                                                                                                                                                                                                                      >  1:132990/1‑297 (MQ=255)             gCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCNCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACNCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                                                                                                                                                                      >  2:709427/1‑300 (MQ=255)                cATTACTGCCGTCTGAGCAAACGGCAATCGTTTATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCGAACGAAACCGGAGCCTATTCCAGTTGCggg                                                                                                                                                                                                                                                                                   >  2:51453/1‑300 (MQ=255)                    acggCCGTCTGAGCAAACGGCAGTCGTCCATCCTGCGCTACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCATTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCGTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACACTCACCACCTGGCTGATACGGCAGTCAAACGAAACCTGAGCCTCTTCCAGTTGCGGGCATc                                                                                                                                                                                                                                                                               <  2:273103/297‑1 (MQ=255)                              gAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGANGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCAcccc                                                                                                                                                                                                                                                                     <  1:539895/300‑1 (MQ=255)                              gAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCAcccc                                                                                                                                                                                                                                                                     <  1:482915/300‑1 (MQ=255)                                                                     tctctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCGCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATggg                                                                                                                                                                                                                              >  2:645967/1‑300 (MQ=255)                                                                     tctctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCACGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATggg                                                                                                                                                                                                                              >  2:424602/1‑300 (MQ=255)                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCAGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGt                                                                                                                                                                                                                           >  1:171708/1‑300 (MQ=255)                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTACTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGt                                                                                                                                                                                                                           >  1:242201/1‑300 (MQ=255)                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCAATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCCAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTATTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCAGTGTTCCATGGGCGt                                                                                                                                                                                                                           >  2:32022/1‑300 (MQ=255)                                                                                                           ccGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGcc                                                                                                                                                                                        <  1:764516/300‑1 (MQ=255)                                                                                                                      cATCAGCTGTCTACTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGCACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCACAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgt                                                                                                                                                                             >  2:353988/1‑300 (MQ=255)                                                                                                                      cATCAACTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgt                                                                                                                                                                             <  2:446219/300‑1 (MQ=255)                                                                                                                             tGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTa                                                                                                                                                                      <  1:708656/300‑1 (MQ=255)                                                                                                                              gTCTCCTTAAGGAGGTTAACAAGCAGGGCGCGTCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                                                                                                                     <  2:20926/300‑1 (MQ=255)                                                                                                                              gTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATAAGGCAGTCAAACGAAACCGGAGCCTATTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac                                                                                                                                                                     >  2:729287/1‑300 (MQ=255)                                                                                                                                ctcCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGCCGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                                                                                                                   <  2:735955/300‑1 (MQ=255)                                                                                                                                ctcCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac                                                                                                                                                                   <  1:503809/300‑1 (MQ=255)                                                                                                                                  ccTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacacac                                                                                                                                                                 >  1:120207/1‑300 (MQ=255)                                                                                                                                    ttAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGAGCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAg                                                                                                                                                               <  1:708590/300‑1 (MQ=255)                                                                                                                                     taatgangTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACNCCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGc                                                                                                                                                              <  2:628468/296‑1 (MQ=255)                                                                                                                                           ggTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa                                                                                                                                                        >  1:21817/1‑300 (MQ=255)                                                                                                                                           ggTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTACTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa                                                                                                                                                        >  1:22392/1‑300 (MQ=255)                                                                                                                                                                    catGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAANCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAgacgg                                                                                                                               >  1:545411/3‑300 (MQ=255)                                                                                                                                                                                      aCCACANCAGCCCTTAGGGTGTGTTGTGACGATGAATCGCTTCGATGGCGCAAANCAGAATGTCGTGGGTGCCGACGCTCACCACCTTGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAAGGCCGGCCAGACGGACGCCCCACGATTCAGGc                                                                                                             <  2:626964/300‑1 (MQ=255)                                                                                                                                                                                      aCCACANCAGCCCGTAGTGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAANCAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGc                                                                                                             <  2:626774/300‑1 (MQ=255)                                                                                                                                                                                       ccACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGca                                                                                                            <  1:583252/300‑1 (MQ=255)                                                                                                                                                                                              aGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGt                                                                                                     >  1:670868/1‑300 (MQ=255)                                                                                                                                                                                                cccGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTaa                                                                                                   <  2:273850/300‑1 (MQ=255)                                                                                                                                                                                                  cGTAGGGTGTGGTGTGACGATGAGTCGCGTCGATGGCGCAAAACAGAATGTCGTGGGTGCCTACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCATCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCattaccc                                                                                                 <  2:730747/300‑7 (MQ=255)                                                                                                                                                                                                  cGTAGGGTGTGGTGTGACGATGAAGAGCTGCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTTGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCTGCTCCTTCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTaggg                                                                                                 <  2:351088/300‑4 (MQ=255)                                                                                                                                                                                                      gggTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCCGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTggg                                                                                             >  2:272151/1‑300 (MQ=255)                                                                                                                                                                                                                 gACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCGGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGGTCGATTTGCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTAGCgg                                                                                  <  1:112977/300‑1 (MQ=255)                                                                                                                                                                                                                       gAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCacac                                                                            <  2:416506/300‑1 (MQ=255)                                                                                                                                                                                                                                     gcgcAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTg                                                              >  1:589960/1‑300 (MQ=255)                                                                                                                                                                                                                                          aaaCAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCACGTCTGCCAGGGGGCGGCGGCAAAGCGGTGTTCCATGGGTGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGt                                                         >  2:637142/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 cGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGTTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCNTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGNGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGAc                                  <  2:607364/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             ggCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGa                      >  1:349700/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCNAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTGACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAatat                <  2:663975/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAAACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGAccgc         <  2:734215/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGNCTCCTGCCCGGCGCTAAGTGGATTTACACACAGCGTTCGAGTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCATTAATGTGGGCGGCGTATCGGTCACACTGCAAACTGCGCTGGCGGTGAACTCGGCGCGCCCGGCTGGACCGTCCGTGGTGAGAATAGGGACCGCCGCGccc  <  1:524798/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      CGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCC  >  NC_000913/1069720‑1070307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑