Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,884	G→A	H157H (CAC→CAT)	rutF ←	FMN reductase RutF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,884	0	G	A	100.0%	176.0 / NA	49	H157H (CAC→CAT)	rutF	FMN reductase RutF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/29);  total (20/29)

TAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGA  >  NC_000913/1069592‑1070157                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                  tAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTANTGCCGTCTGAGCAAACGGCAATCGTTNATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAAc                                                                                                                                                                                                                                                                            <  2:508065/300‑1 (MQ=255)   acgggggACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTg                                                                                                                                                                                                                                                                          <  1:699855/297‑1 (MQ=255)   aCTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGGACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCANCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTNACAAGCAGGGCGCATCAGCGCATGATAACTg                                                                                                                                                                                                                                                                          <  2:537032/300‑1 (MQ=255)                           aaaGAAAAACAGTAACATGCCAATCCCCGACATGAAAATGGGAAGACTGGGATCCAGCCCCATCAGAGTCGGCACGAACACCGTCGCGCCAAGCATCGCCACCGCTTGGTGAATGCCCATTACTTCCTTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCACTCTCTGTAGAGGTCGATTTTAACGGCCAGTTAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAGCTGCAATCGAACCACACCACCCCGTAg                                                                                                                                                                                                                                                  <  2:313790/300‑1 (MQ=255)                            aaGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCAGTAgg                                                                                                                                                                                                                                                 >  1:621340/1‑300 (MQ=255)                             aGAAAAACATTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCAGCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAACAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAggg                                                                                                                                                                                                                                                <  2:388685/300‑1 (MQ=255)                             aGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGTATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAggg                                                                                                                                                                                                                                                <  2:73145/300‑1 (MQ=255)                             aGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGNACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAggg                                                                                                                                                                                                                                                <  1:541826/300‑1 (MQ=255)                             aGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAggg                                                                                                                                                                                                                                                <  1:15944/300‑1 (MQ=255)                                 aaaCAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtg                                                                                                                                                                                                                                            >  1:679314/1‑300 (MQ=255)                                   aCAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCAATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtggt                                                                                                                                                                                                                                          >  2:586252/1‑300 (MQ=255)                                    cAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATNAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCANGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtggtg                                                                                                                                                                                                                                         <  2:706933/300‑1 (MQ=255)                                     aGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtggtgt                                                                                                                                                                                                                                        <  1:24736/300‑1 (MQ=255)                                     aGTAACATGCCGATCCCCGACATTAAAATGGAAGGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtggtgt                                                                                                                                                                                                                                        <  2:584712/300‑1 (MQ=255)                                       tAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGa                                                                                                                                                                                                                                      <  2:449626/300‑1 (MQ=255)                                        aaCGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGAc                                                                                                                                                                                                                                     >  1:646014/1‑300 (MQ=255)                                                                                        aTCAGAATCGGCATTAAAACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTTCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGTAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGc                                                                                                                                                                                     <  2:435304/300‑1 (MQ=255)                                                                                               tCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGTTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGACGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTcaccac                                                                                                                                                                              <  2:6781/300‑1 (MQ=255)                                                                                                    aTTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGc                                                                                                                                                                         <  1:123561/300‑1 (MQ=255)                                                                                                          acCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATAc                                                                                                                                                                   <  1:166538/300‑1 (MQ=255)                                                                                                             gTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGc                                                                                                                                                                <  1:316698/300‑1 (MQ=255)                                                                                                                gcgcCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCAGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGt                                                                                                                                                             >  2:328044/1‑300 (MQ=255)                                                                                                                                 cgcgTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGcc                                                                                                                                            >  1:464934/1‑300 (MQ=255)                                                                                                                                            cGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTg                                                                                                                                 <  1:753679/300‑1 (MQ=255)                                                                                                                                             gCCCATTACTGCCGTCTGAGCAAACGGCCATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                >  2:283718/1‑300 (MQ=255)                                                                                                                                             gCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGCAGGGTGCGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCCGTCAAACGAAACCGGAGCCTCTTCCAGtcgc                                                                                                                                >  1:132990/1‑297 (MQ=255)                                                                                                                                             gCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCNCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACNCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGc                                                                                                                                >  2:709427/1‑300 (MQ=255)                                                                                                                                                cATTACTGCCGTCTGAGCAAACGGCAATCGTTTATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCGAACGAAACCGGAGCCTATTCCAGTTGCggg                                                                                                                             >  2:51453/1‑300 (MQ=255)                                                                                                                                                    acggCCGTCTGAGCAAACGGCAGTCGTCCATCCTGCGCTACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCATTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCGTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACACTCACCACCTGGCTGATACGGCAGTCAAACGAAACCTGAGCCTCTTCCAGTTGCGGGCATc                                                                                                                         <  2:273103/297‑1 (MQ=255)                                                                                                                                                              gAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGANGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCAcccc                                                                                                               <  1:539895/300‑1 (MQ=255)                                                                                                                                                              gAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCAcccc                                                                                                               <  1:482915/300‑1 (MQ=255)                                                                                                                                                                                                     tctctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCGCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATggg                                                                        >  2:645967/1‑300 (MQ=255)                                                                                                                                                                                                     tctctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCACGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATggg                                                                        >  2:424602/1‑300 (MQ=255)                                                                                                                                                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCAGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGt                                                                     >  1:171708/1‑300 (MQ=255)                                                                                                                                                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTACTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGt                                                                     >  1:242201/1‑300 (MQ=255)                                                                                                                                                                                                        ctGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCAATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCCAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTATTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCAGTGTTCCATGGGCGt                                                                     >  2:32022/1‑300 (MQ=255)                                                                                                                                                                                                                                           ccGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGcc                                  <  1:764516/300‑1 (MQ=255)                                                                                                                                                                                                                                                      cATCAGCTGTCTACTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGCACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCACAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgt                       >  2:353988/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cATCAACTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAgt                       <  2:446219/300‑1 (MQ=255)                                                                                                                                                                                                                                                             tGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTa                <  1:708656/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gTCTCCTTAAGGAGGTTAACAAGCAGGGCGCGTCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac               <  2:20926/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATAAGGCAGTCAAACGAAACCGGAGCCTATTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTac               >  2:729287/1‑300 (MQ=255)                                                                                                                                                                                                                                                                ctcCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGCCGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac             <  2:735955/300‑1 (MQ=255)                                                                                                                                                                                                                                                                ctcCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacac             <  1:503809/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  ccTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTacacac           >  1:120207/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    ttAAGGAGGTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGAGCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAg         <  1:708590/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     taatgangTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACNCCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGc        <  2:628468/296‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa  >  1:21817/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           ggTTAACAAGCAGGGCGCATCAGCGCATGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCGGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTACTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGa  >  1:22392/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                  TAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGGCTGATACGGCAGTCAAACGAAACCAGAGCCTCTTCCAGTTGCGGGCATCCGGTCACCCCCGTCTGCCAGCGGGCGGCGGCAAAGCGGTGTTCCATGGGCGTTTTGCCGCCAAAAAGGTTTGAAAGCGGCTCCTGCCCGGCGCTAAGTGTATTTACACACAGCGTTCGA  >  NC_000913/1069592‑1070157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑