Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,008,711	G→A	D290N (GAT→AAT)	rlmL →	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,008,711	0	G	A	100.0%	190.6 / NA	54	D290N (GAT→AAT)	rlmL	fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/27);  total (27/27)

GCTCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAGATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGAC  >  NC_000913/1008422‑1008998                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                gctCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCa                                                                                                                                                                                                                                                                                       >  2:736005/1‑300 (MQ=255)                       ggTATGTTGCTGATTTAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTTAAAAATGTCGCGCAACTGACCAATCCGCTGCCGaaa                                                                                                                                                                                                                                                                 <  2:243063/300‑1 (MQ=255)                       ggTACGTTGCTGATTGAAGCAGCGATGTTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGaaa                                                                                                                                                                                                                                                                 <  2:354285/300‑1 (MQ=255)                            gTTGCTGATTGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGcc                                                                                                                                                                                                                                                            >  1:680588/1‑300 (MQ=255)                             ttGCTGATTGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCg                                                                                                                                                                                                                                                           >  1:711865/1‑300 (MQ=255)                                     ttaatcagcGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATNGCTCTCATTTTTACGGTTCGGACAGCNACGCACGGGTGGTTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGTAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGcac                                                                                                                                                                                                                                                   <  2:512978/295‑1 (MQ=255)                                     tGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGTGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGGAAAGGCCTGGCTGAGTATAGCCCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACACACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGGGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGcac                                                                                                                                                                                                                                                   <  2:167352/300‑1 (MQ=255)                                     tGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGcac                                                                                                                                                                                                                                                   <  1:219493/300‑1 (MQ=255)                                       aaGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCAGAAAGGGCCGTACGGCACAg                                                                                                                                                                                                                                                 >  1:270908/1‑300 (MQ=255)                                          cagcGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTTGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCACAAACTCGCGCCCGTAAAGGCCTGACTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTACACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAACGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAgtgt                                                                                                                                                                                                                                              <  2:22961/300‑1 (MQ=255)                                               aTGCTTGCGACCGATCGCGCACCATGCTTGCACCGTGTTCGTTTGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTCGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGTGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGc                                                                                                                                                                                                                                         <  2:455020/300‑1 (MQ=255)                                               aTGCTGTCGACCGATCGCGCACCATGCTTGCACCGTGGGCGTTTGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGNAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGc                                                                                                                                                                                                                                         <  2:672395/300‑1 (MQ=255)                                               aTGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTTGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGc                                                                                                                                                                                                                                         <  2:57717/300‑1 (MQ=255)                                               aTGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGTTGAAAAATTTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGGACGGCACAGTGTTGAGc                                                                                                                                                                                                                                         <  2:82077/300‑1 (MQ=255)                                                 gCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCaa                                                                                                                                                                                                                                       >  2:36531/1‑300 (MQ=255)                                                    ggCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAAccc                                                                                                                                                                                                                                    <  1:491426/300‑1 (MQ=255)                                                             tCGCGCACCAGGCTTGCACCGTGGGCGTTTGTGCTTTAGCGGCTGGGCGCAGCATAATGAAACTATCCGGCAGGAATTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTTAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGTTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACgg                                                                                                                                                                                                                           <  2:37485/300‑1 (MQ=255)                                                                  cACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAAc                                                                                                                                                                                                                      >  2:579266/1‑300 (MQ=255)                                                                       ggCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTg                                                                                                                                                                                                                 >  2:292035/1‑300 (MQ=255)                                                                       ggCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTg                                                                                                                                                                                                                 >  1:461512/1‑300 (MQ=255)                                                                       ggCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCATGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGACACACGGGGAACGTCTg                                                                                                                                                                                                                 >  1:232067/1‑300 (MQ=255)                                                                          tggaaCNGTGGGCGTTGTGCCTTTAGCGGCGGGGCGCAGCATGATTAAGCTATCNGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGTACAGCGACGCACGGGTGATTCAACGTACACACACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACGGACCAATCCGCGGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGAc                                                                                                                                                                                                              <  2:623858/296‑1 (MQ=255)                                                                            gCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAg                                                                                                                                                                                                            <  1:753988/300‑1 (MQ=255)                                                                                       gTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGgcgc                                                                                                                                                                                                 >  1:21139/1‑300 (MQ=255)                                                                                                      gCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATNCAACGTGCACGCACTAACGCCCGTCTNGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATa                                                                                                                                                                                  >  2:522743/1‑300 (MQ=255)                                                                                                         gggCGCAGCATGATGAAGCTATTTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGCCTTGCGGGGATTGGTGAACTAATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGcc                                                                                                                                                                               <  2:269061/300‑1 (MQ=255)                                                                                                         gggCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGcc                                                                                                                                                                               <  1:17146/300‑1 (MQ=255)                                                                                                          ggCGCAGCATGATGAAGCTATCTGGCAGGAGGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAGTCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCct                                                                                                                                                                              <  2:111615/300‑1 (MQ=255)                                                                                                                gcaTGATGAAGCTATCTGGCAGGAAGTGAAANCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCANGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGCCAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTggg                                                                                                                                                                        >  2:526772/1‑300 (MQ=255)                                                                                                                 caTGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGt                                                                                                                                                                       <  1:333811/300‑1 (MQ=255)                                                                                                                   tgatgaAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCg                                                                                                                                                                     >  1:100424/1‑300 (MQ=255)                                                                                                                      tgaAGCTATCTGGCAGTAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAGTCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGAt                                                                                                                                                                  <  1:588960/300‑1 (MQ=255)                                                                                                                      tgaAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGAt                                                                                                                                                                  <  1:760368/300‑1 (MQ=255)                                                                                                                                         gTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTtggt                                                                                                                                               >  1:351400/1‑300 (MQ=255)                                                                                                                                                                                gCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCCTAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGCTCTg                                                                                                        >  2:401890/1‑300 (MQ=255)                                                                                                                                                                                     gTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATAAGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGCTCTGCTAAg                                                                                                   >  2:139751/1‑300 (MQ=255)                                                                                                                                                                                      tataGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCANGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAANACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAgc                                                                                                  <  2:694503/300‑1 (MQ=255)                                                                                                                                                                                                aTTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGc                                                                                        <  2:424435/300‑1 (MQ=255)                                                                                                                                                                                                        ggTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCa                                                                                >  2:493049/1‑300 (MQ=255)                                                                                                                                                                                                             ggACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGTTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACaa                                                                           >  1:451081/1‑300 (MQ=255)                                                                                                                                                                                                             ggACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACaa                                                                           >  1:213236/1‑300 (MQ=255)                                                                                                                                                                                                                    gACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTAc                                                                    <  1:451552/300‑1 (MQ=255)                                                                                                                                                                                                                     aCGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACa                                                                   >  1:454381/1‑300 (MQ=255)                                                                                                                                                                                                                                            cgcacTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGAc                                            <  1:80317/300‑1 (MQ=255)                                                                                                                                                                                                                                              cacTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTg                                          >  1:160010/1‑300 (MQ=255)                                                                                                                                                                                                                                              cacTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTg                                          >  2:473281/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cccGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGa                                  <  1:645648/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTNACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTNCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGaaa                                >  2:708497/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ttGGTGAACTGATCACCTTTGCTGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTg                   >  1:623840/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ggTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCNGATTGCGCTGCATAGCCTGCTGGGTCNGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGcc                 >  2:521349/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ggTGAACTGATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCATACGGCACAATGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGTGCTGCTTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTAGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTCCACGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCaagctgca                 >  2:16868/1‑293 (MQ=255)                                                                                                                                                                                                                                                                               gATCACCTTTGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGCCGAAAGCAc         >  1:734237/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ttttaGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGAc  <  1:124867/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tttGAGGTGAAAAATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGAc  <  1:625405/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                GCTCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCAGCGATGCTGGCGACCGATCGCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAAGCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGTGATTCAACGTGCACGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAGATGTCGCGCAACTGACCAATCCGCTGCCGAAAGGGCCGTACGGCACAGTGTTGAGCAACCCGCCATACGGGGAACGTCTGGACAGCGAACCGGCGCTGATTGCGCTGCATAGCCTGCTGGGTCGGATCATGAAAAACCAGTTTGGTGGCTGGAATCTCTCTTTGTTTAGTGCCTCGCCGGATCTGCTAAGCTGCTTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGAC  >  NC_000913/1008422‑1008998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑