| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,054,886 | 0 | G | T | 28.2% | 149.4 / 22.5 | 86 | Q187K (CAA→AAA) | ygfB | PF03695 family protein YgfB |
| Reads supporting (aligned to +/- strand): ref base G (18/43); new base T (24/0); total (43/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAA > NC_000913/3054590‑3055181 | aGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAc < 1:61270/300‑1 (MQ=255) aaCGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACNAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGgcg < 2:517344/300‑1 (MQ=255) cgGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGTATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTGGCGcggt > 1:667642/1‑300 (MQ=255) gTTAAACAGAACACTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCAACAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGtccctcttgctctgtcg > 1:567686/1‑284 (MQ=255) aCAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGc < 1:190974/300‑1 (MQ=255) aCAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCAGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGCGCGGTCGGTTGc > 1:55324/1‑300 (MQ=255) cGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCATTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTGgcgtgggtggttgtgggtgg > 2:630379/1‑283 (MQ=255) ggTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGNCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTNTAGTGTAGAGGTGGTTTTTTTACTTCTTGCGCGGTTGGTTGTGGATGAGTaaaa > 2:638288/1‑299 (MQ=255) ggTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCAGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCAGGGATATCTCACTCATAACACTCTACTTACGTTTTTTGTTTTTAGTGTAGAGTGGGTTTTTGTACTTCTTGCGAGGTCAGTTGCGGATGAGtccaa > 1:228226/1‑296 (MQ=255) ggTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTACGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGTTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGGTTTTGTACTTCtcacaaagtcggtttctgatgggtcaag > 2:193498/1‑273 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGTGGAAGTACCAGAAGTCACGGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACGTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGGCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAgg < 2:198609/300‑1 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTCCCTCTGGCGCGGTTTGTTTCGGATGAGTaaaag > 2:89221/1‑298 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAgg < 1:107406/300‑1 (MQ=255) gTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCNGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTTTTGCGGTGTCGGTTGCTGATGAGTAAAgg > 1:490330/1‑300 (MQ=255) gAGTGTCATCGCTTTTAATCCGCACCAGCCCCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGAGTTGCATTTGCTCCAACAGGGCCTGACGGCGAAGCTGAAACTCTTGCAGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTCGAGTTGGTTTTTCTGCTTCTCGCGATGTCGGGTTCGGATGAGTAAAAGTGTCg > 1:187735/1‑300 (MQ=255) tgtCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATGCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg < 1:39597/300‑1 (MQ=255) gtCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCANAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAANTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCCTCTTGCGCGGTTGGGTGCGGACGAGTAAAAGTGTCGttgg > 2:545202/1‑297 (MQ=255) cGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAAc < 1:108330/300‑1 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGNTATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCCTCTTGCGCGGTCAGTTTCGGATGAGTAAAGGTGTCGTGGCAtcaaag > 1:505294/1‑295 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCTGTTTTTGTCCTTCTGGTGCGGTCGGTTTTGGAGGAGTAAAGAGGTCGTGGCATAACAg > 2:348083/1‑300 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCCTCTTGCGCGGTCTGTTTCGGATGAGTAAAGGTGTCGTGGCATAACaa > 1:5756/1‑299 (MQ=255) cTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTATTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAg < 1:583244/300‑1 (MQ=255) caccagcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCCTCTTGCGCGGTTGGTTTCGGGTGAGTAAAGGTGTCGTGGAACAACAACGCGGCAacac > 2:386232/1‑300 (MQ=255) caccagcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTTCGGATGAGTAAAAGTGTCGTGGCATAACAACGCGGCAacac > 1:16584/1‑300 (MQ=255) caccagcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAacac < 1:182236/300‑1 (MQ=255) gcaccGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGAAGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACg < 1:638668/300‑1 (MQ=255) gCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTGCGCGGTCTGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAGCGCGGCAACAAAAACGTATTCA‑ATGAtctcctccaacg > 1:404314/1‑292 (MQ=255) gCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCGACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGGTTTTTTACCTCCGGCTCAGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAAAAACGCAGCAacacaaaaatattcgccgagttcctccagca > 2:433786/1‑273 (MQ=255) aGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCa < 1:334555/300‑1 (MQ=255) gTACCAGAAGTCACTGTTCTGACGATAGGGGNATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGANGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACCTCTGGTGCGGTCGGGTGCGGATGAGTAAAAGTGTCGTGGCATCACAACCCGGCAACACAAACATACTCG‑ATGAGctctcccaaagacacttcca > 2:481552/1‑284 (MQ=255) cTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGAtc < 1:335688/300‑1 (MQ=255) cTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCAACAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACTTCTGGTGTAGTCGGTTTAAGGTGAGTCAAAGTGGCGTGGAATAAACGCACGGCAACAAAAACAGATTCG‑AGGATCTCTTCCAGAGGCA‑ATTCAAGATCTTCCTGagc > 1:1173/1‑298 (MQ=255) gTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTTTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAGCGCGGCAACACGAACATATTCG‑ATGATCTCTTCAAGCGACA‑CTTCCAACTCTTCCTGAtctt > 1:23381/1‑300 (MQ=255) cTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGAtcttcgt < 2:174152/300‑1 (MQ=255) cGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTGCTTCTTGTGCGGTCGGTTTCTGGTGAGGGAAGGGGTCGTGGACTCACCACTCGGGAAAACAAACGTATTCG‑ACGAGTTCTTCCAGCGACACTTTCAA‑CTCTTCCCGAtcttcgtcttt > 2:305712/1‑299 (MQ=255) gTATTCGCTGTCGGCGCTAAGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACTTTTTGTGCGGTGGGTTGTGGGTGAGGAAAGGTGTCGTGGAATAAAAGCGCGGAAACACGGAAGTATTCGGCTG‑CCTCTTCAAGCGAAA‑TTTCAAGCTCTTTTTGATCTTCGTTTTCGTTGTaaa > 2:3269/1‑299 (MQ=255) ggCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGNGTCGGTTTTTGTACTTCTGGCGCGGTNGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa < 2:475676/300‑1 (MQ=255) gcgcTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGTGCGGTTGGTTTTGGGTGAGTAAAGGTGTCGTGGCATAAAAACGCAGCAACACGAACGTACTCG‑ATGATCTCTTCCAACGACA‑CTTCCAGCCCTTCCCTATCTTCGTCTTCGTCGTAAACCAAGTGCGCaac > 1:361405/1‑299 (MQ=255) cGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGAGGTTTTTTTACCTCCGGCGAAGTCGGTTGCGGATGAGTAAAAGTGTCGTGGAATAAAAGCGCGGCAACAAGAACGTATTTG‑AGGATCTCTTCCAAAGAAA‑CTTCCAACTCTTCCTGGGCTTCTTCTTCGTCGTAACCCAATTgtggaatggtaca > 1:9016/1‑288 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCNGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTNTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCTTCTTGCGCGGTCGGTTGTGGATGAGTAAAAGTGTCGTGGCATAACAACACGACAAAACAAACATCTTCG‑CCGATCTCTTCCAACGACA‑TTTCCAACTCCTCCTTATCTTCCTCTTCATCATAAACCAGtcgctccaccttacgc > 2:481418/1‑285 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTTTCCCTCTTGCGCGGTCGGTTTTGGGTGAGTACAAGTGTCGTTGCACCACAACACGGCAACACAAACATCTTCG‑ACTCTCTCTTCCAACAACA‑TNTCCCCCTCCTCCCTATCTTTGTCTTCGTTGTAACACAATTGCGCaagcgtccaa > 2:517488/1‑292 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCGGATGAGTAAAGGTGTCATGGCACCACAACACGGCAACACAAACGTATTTG‑ATGATCTCTTCCAACAACA‑CTTCCAGCTCTTCCTTATCTTCGTCTTCGTCGTAAACCAATTGTGAAATGTTACGc > 1:532502/1‑300 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 1:85665/300‑1 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTTTCCTTCTTGTGCGGTCGGTTGCGGATGAGTAAAAGGGTCTTGGCATCACAACACAGAAACACAAACATCTTCT‑ATGGCCTCTTCAAACAACA‑CTTCAAACCCTTCCTTATCTTCGTCTTcgtcgtcaacaaattgctcaaagttccgc > 1:632971/1‑277 (MQ=255) gtgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCANAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:599450/300‑1 (MQ=255) gtgtTACGTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACTGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc < 2:300802/300‑1 (MQ=255) gggtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGAGCGGTCGGTTGCGGAGGAGGAAAGGTGTCGTGGCATAAAAACACGGAAAAACGAAAATATTTG‑ATGGTCTCCTCAAACAAAA‑TTTCACACTCTTCCTGGTCTTCGTCTTTGTCGTAACCCAGGTGTGAAACGTTACGc > 1:76977/3‑300 (MQ=255) tgtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCCTGCGCGGTCGGTTTCTGAGGAGGAAAGGTGTCGTGGCACCACAACACAGAAACAAAAACGTATTCT‑ATGATCTCCTCAAACGACC‑TTTCAAGCTCTTCCCTATCTTCATTTTCGTCTTAAACCAGCTGCGCAATGTTAcaca > 2:277015/1‑297 (MQ=255) gtTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAg < 1:586523/300‑1 (MQ=255) gtTACTTCTGATGCAGCAAAAATCAGCGCGGCGCTGCCTGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAg < 2:99609/300‑1 (MQ=255) gcagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTTCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTCCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGc < 1:175597/300‑1 (MQ=255) cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCt < 2:399908/300‑1 (MQ=255) aaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAcc < 1:315795/300‑1 (MQ=255) tCAGCGCTGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTTAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTACTNCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGtt < 2:165551/300‑1 (MQ=255) gcgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTTGGTTGCGGATGAGTAAAAGTGTCATGGCACAACAACGCGGCAAAACAAACATACTCG‑ATGATCTCCTCAAACGACA‑CTTCCAGCTCTTCCCGATCTTCATCTTCGTCGTAAACCAAGTGTGCAACGTTCCACAAAGCGTCGATAGCTTCACCAGtttttccggt > 1:96389/1‑293 (MQ=255) ccGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑ttt < 1:101229/300‑1 (MQ=255) gggTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGGCGGTTTTTGTCCTTCTTGCGCGGTCTGTTGCGGGTGAGTAAAGGTGTCGTGGCATCACAGCGCGGCAACACGAACTTCTTCT‑ATGATCTCCTCCAGCGACA‑TTTCAAACTCTTCCTGATCTTCGTCTTTGTCGTAGCCCAGTTGCGTAACGTTACACGAATCGTCGATCGTGTCACCGGTTTTGTCGGTCAC‑TTTAt > 2:66837/1‑300 (MQ=255) gTTGCATTTGCTCCACCAGGGCCTGACGGCGGCGCTTAAACTCTTGCCTGGATATCTCACTCACAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCGGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCCGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGCTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATcc < 2:448031/300‑1 (MQ=255) gTTGCATTCGCTCTACCAGGGCCTGACGGCGACGCTGAAACTCTTGTCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGGTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATcc < 1:53997/300‑1 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGc < 1:439606/300‑1 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTTGGTTTTTGTACTTCTTGTGCTGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGAAACACCAAAGTATTTG‑ATGATCTCCTCCAGCGACC‑TTTCCAACTCTTCCTTATCTTCTTCTTCGTCGTCACCAAATTTCGCAACGTTCCGCAAACCGTCGATCGCTTCACCAGTTTTTCCGGTCAccttcctccgactctg > 1:254497/1‑285 (MQ=255) gCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTNCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGt < 2:596906/300‑1 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACCTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCACAACAACGCGGCAACACAAACGTATTCG‑ATGATCTCTTCAAGCGAAA‑TTTCAAGCTCTTCCTGGTCTTCGTCTTCGTCGTAACCCAATTGCGAAATGTTCCGCAGATCGTCGATAGCTTCCCCAGGTTCGACAGTCCC‑TTTATCCAGCTTCGGTTgtgt > 1:96730/1‑297 (MQ=255) accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTCTTAGTGTAGAGTCGGTTTTTGTCCTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATCACAGCGCGGCAACACGAACGTCTTCG‑ATGATCTCTTCCAGCGACA‑CTTCAAACTCTTCCTGATCTTCGTCTTCGTCGTAAACCAATTGTGCAACGTTCCGAAGATCGTCGATAGCTTCAACGGTTTCGCCGGTCAA‑TTTCTCCCGCTTCGGTTGCGt > 1:247321/1‑300 (MQ=255) ggCCTGACGGCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGc < 1:611254/300‑1 (MQ=255) actgCGACGCTGAAACTCTTGCCTGGATATCTCACTCATAACACTCTCCCTACTTTTTTTTTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGAc < 2:64293/297‑1 (MQ=255) aCGGCGATGCGGAAACCCTTTCCTGGATAACTCACGCATAACACTCGCCTGACGTTTTTTGTTTTTAGTGGAGAGTCGTTTTTTGTACTTCTTGCGCGGTCGGTTGCGGATGATTAAAGGTGTCTTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGTTTGCTTAACGCCAAGAc < 2:325228/300‑1 (MQ=255) aCGGCGACGCTGAAACTCTTTCCTGGATATCTCACTCATAACACTCTCCTTACGNTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACGGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGAc < 2:518664/300‑1 (MQ=255) aCGGCGACGCGGAAACTCTTGCCGTGATATCTCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGGCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGAc < 2:76123/300‑1 (MQ=255) ctctTGNCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTNTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGAt < 2:572421/300‑1 (MQ=255) tGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCGGATTAGTAAAGGTGTCGTGGCATCACCACGCAGCCACACAAACGTCCTCG‑ATGACCTCTTCAAGAGAAA‑CTTCAAACTCTTCCTGATCTTCGTCTTCGTCGTCAACCAATTGTGCAATGTTACGCAAATCGTCGATCGCTTCCCCGGTTTTGCCGGTCAC‑TTTCTCCCGCTTCGGTTTCGTAACACCAAGACCAAGAAGGaaatggctgaa > 1:357710/1‑291 (MQ=255) tGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGAc < 1:597528/300‑1 (MQ=255) gcctggATATCTCACTCATAACACTCTCCTTACTTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCTTAACGCCAAGACCAAGCAGGAAGTGATTGAcc < 1:646908/296‑1 (MQ=255) gCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGAcc < 1:532000/300‑1 (MQ=255) gATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTTTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAAcc < 1:293270/300‑1 (MQ=255) gATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAAcc < 1:292810/300‑1 (MQ=255) atCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTGGCGCGGTCAGTTTCGGATGAGTAAAGGTGTCGTGGCCCCACAACACAGCAACACAAACGTCTTCG‑ATGATCTCCTCAAACGACA‑CTTCCAGCTCTTCCCGATCTTCATCTTCGTCGTAACCCCGTTGCGCAACGTTACGCAGATCGTCGATAGCTTCCCCAGTTTTGCCGGTCCC‑TTTATTCCGCCTCAGTTGTGTAACACCAAAAACAAGCAAGAAGTGATTGAACCAACCttc > 1:638970/1‑298 (MQ=255) atCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCCTCCTGCGCAGTTGGTTTCGGACGAGTAAAAGTGTCACGGCCTCACAACACGGAAACACAAACATATTCC‑ATGATCCCTTCCAGCGACA‑TTTCCAGCTCTTCCTGATCTTCGTCTTCGTCGTAAACCAGTTGTGCAACGTTACACAAATCGTCGACAGCTTCCCCGGTTTCTCCGGTCAC‑CTTCTCCAGCTTCGGTTGCGTAAAGCCAAGACCAAGCAGGAAGTGATTGGCCCCACCTGc > 1:543039/1‑300 (MQ=255) actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCGGTTTCGGATGAGTAAAAGTGTCGTGGCATAACAACACAGCAACACAAACATATTTT‑ATGATCTCTTCCAACGACA‑CTTCCCACTCTTCCTTATCTTCGTCTTCGTCGTCACCCAATTTCGCAATGTTCCGCAGATCGTCGATCGCTTCCCCAGTTTCGCCGGTCACCTTCTTCCA‑CTTCGGTTGCGTAACAACAAAAACAAACAAGAAGTGATTTACCCCACCTTCCaacg > 1:608765/1‑298 (MQ=255) ctcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTCCTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAACACGGCAACACAAACATACTCT‑ATGATCTCTTCAAACAACA‑TTTCCAGCTCTTCCTTATCTTCGTCTTCGTCGTCAACCAGTTTCGCAATGTTCCGCAGATCGTCGATAGCTTCACCAGTTTCTCCAGTCAC‑TTTCTCCCACTTCGGTTTCTTCACACAAAAACCCAACAGGAAGTGGTTTACCCAAccctcccccgc > 1:556961/1‑291 (MQ=255) caTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTCCTTCTTGCGCGGTCTGTTTCTGACGAATAAAAGTGTCGTGGCACCACAACACAGCAACACAAAAATCTTCT‑ATGATCTCCTCCAACGACA‑CTTCAAACTCTTCCCTATCCTCGTCTTCGTCGTAAACCAATTTTGAAACGTTCCGCAGATCGTCGATAGCTTCACCGGTTTCGACAGTCAC‑TTTCTCCAACTTCGGTTGTGTAAAGACAAAAACAAGCAGGAAGTGATTGAACCaaacctccaatgtat > 1:555250/1‑287 (MQ=255) aaCACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAg < 1:345494/300‑1 (MQ=255) cacTCCCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGcc < 2:96078/300‑1 (MQ=255) aCGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCNCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATNTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGaaaa < 2:544232/300‑1 (MQ=255) gtgtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCCAGCTCTTACTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGAACCAACCTGCCAATGCATCAGCACGATCGAAAACGCTGACATCATCGcc > 2:208914/1‑300 (MQ=255) ttctGTACTTCTGGCGCGGTTGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAGCGTGTTCG‑ATGATCTCTTCAAGCGGCG‑TTTCAAGCTTTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTGCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTACCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATaa < 2:196194/297‑1 (MQ=255) | AGGTCGCGAACGCGGTTAAACAGAACGCTGTGGTTATGAGTGTCATCGCTTTTAATCAGCACCAGCACCGCTTCCGGTTCGTTAAAGCCGGTGAAGTACCAGAAGTCACTGTTCTGACGATAGGGGTATTCGCTGTCGGCGCTACGTGTTACTTCTGGTGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCG‑ATGATCTCTTCAAGCGACA‑TTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCCGGTCAC‑TTTATCCAGCTTCGGTTGCGTAACGCCAAGACCAAGCAGGAAGTGATTGACCCAACCTGCCAATGCATCAGCCCGATCGAAAACGCTGACATCATCGCCATCAGGCAGATAA > NC_000913/3054590‑3055181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |