Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,708,467	Δ168 bp		[proK]	[proK]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3708467	3708634	168	42 [0]	[24] 66	[proK]	[proK]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3708466	0 (0.000)	42 (0.730)	29/382	0.7	100%	intergenic (‑761/+150)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
	?	NC_000913	3708635 =	0 (0.000)					noncoding (58/77 nt)	proK	tRNA‑Pro

CGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGGGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTT  >  NC_000913/3708339‑3708929                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       aagatttaagcaaatataaaaaaagacaatggtttctgtcacaaatccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcgttcgcgccgcatccgacaatggcctgcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGaa                                                                                                                                                                                                                                                                                                     <  2:336627‑M2/4‑1 (MQ=255)      ttaagcaaatataaaaaaagacaatggtttctgtcacaaatccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTcc                                                                                                                                                                                                                                                                                                <  1:592405‑M2/9‑1 (MQ=255)          gcaaatataaagaaagacaattgtttctttcacaaatccgttaatggatagttagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGAcc                                                                                                                                                                                                                                                                                            <  2:380596‑M2/13‑1 (MQ=255)            aaatataaaaaaagacaatggtttctgtcacaaatccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGAcccc                                                                                                                                                                                                                                                                                          <  1:295942‑M2/15‑1 (MQ=255)            aaatagaaaaaaagacaatggttcctgtcacaaaccctttaatggatagtgaaatatggggcgcaaatttggcagggaaatgtgtgtcgttgggttcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttaccaggcctacgtcgggtcgaacctccctgcccccggaggttctcatcctcatgagctgcagaggcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGAcccc                                                                                                                                                                                                                                                                                          <  1:550372‑M2/15‑1 (MQ=255)            aaagataaaaaaagaaaatggtgtctgtcaaaaatccggtaatggatagtgagatagggggcgcaaatgtggcagggaaatgtgtggcgtgtagggcgaataaggcggtcgcgccgcagccgacaatggccggcacatttgactgaggcgacgctgacgcgtcttagcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcataaggagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGAcccc                                                                                                                                                                                                                                                                                          <  1:198792‑M2/15‑1 (MQ=255)                   aaaaaagacaatggtttctgtcacaaatccattaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTcc                                                                                                                                                                                                                                                                                   <  2:199758‑M2/22‑1 (MQ=255)                        agacgatcgtttctgtcacaaatccgttaattgatagtgagatagggggcgcaaattcggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacgtttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcgttgatagaggattCGAACCTCCGACCCCTTCGTCCcgaac                                                                                                                                                                                                                                                                              <  2:176883‑M2/27‑1 (MQ=255)                                 tttctgtcacaaatccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTgcgc                                                                                                                                                                                                                                                                     <  1:84706‑M2/36‑1 (MQ=255)                                         acaaatccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc                                                                                                                                                                                                                                                             <  1:593425‑M2/44‑1 (MQ=255)                                         acaaatccgtgaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcgttgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGc                                                                                                                                                                                                                                                             <  2:177392‑M2/44‑1 (MQ=255)                                           aaatccgttaattgatagtgagatatggggcgcaaatttggcaggtaaatgtgtntcgtgtagttcgaataaggcgttcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTg                                                                                                                                                                                                                                                           <  2:514876‑M2/46‑1 (MQ=255)                                           aaagacgttaatggatagtgagatatggggcgcagatttggcagggaaatgtgtgtcgtgtaggtcgaagaatgcggtcgcgccgcatccgacaattgccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCATGCTg                                                                                                                                                                                                                                                           <  2:62823‑M2/46‑1 (MQ=255)                                              tccgttaatggatagtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcgttgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTgcgc                                                                                                                                                                                                                                                        <  1:312846‑M2/49‑1 (MQ=255)                                                   taatggatagtgagatatggttcgcaaatttggcaggtaaatgtgtgtcgtgtaggtcgaataaggcagtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacggcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggcctgtgatagaggattCGAACCTCCGACCCCTCCGTCCCGAACGGAGTGCGCTACCATGCTGCGccaacc                                                                                                                                                                                                                                                   <  2:521786‑M2/54‑3 (MQ=255)                                                            gtgagatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGc                                                                                                                                                                                                                                          >  2:415667‑M2/238‑300 (MQ=255)                                                                gatatggggcgcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCgggg                                                                                                                                                                                                                                      >  1:180908‑M2/234‑300 (MQ=255)                                                                 acatggggcacaaatttggcagggaaatatgtgtcgtgtaggtcgaataatgcggtcgcaccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcatgcctacttcgagtcgaacctcccttcccccgtaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgc                                                                                                                                                                                                                                     <  2:198188‑M2/68‑1 (MQ=255)                                                                          gcaaatttggcagggaaatgtgtgtcgtgtagttcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTAc                                                                                                                                                                                                                            <  1:154070‑M2/77‑1 (MQ=255)                                                                          gcaaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTAc                                                                                                                                                                                                                            <  1:214024‑M2/77‑1 (MQ=255)                                                                            aaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg                                                                                                                                                                                                                          <  1:517458‑M2/79‑1 (MQ=255)                                                                            aaatttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcacgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg                                                                                                                                                                                                                          <  2:3996‑M2/79‑1 (MQ=255)                                                                               tttggcagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacttcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcgc                                                                                                                                                                                                                       <  1:596851‑M2/82‑1 (MQ=255)                                                                                    cagggaaatgtgtgtcgtgtagttcgaataaggcggtcgcgccgcatccgacaanggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATa                                                                                                                                                                                                                  <  2:516171‑M2/87‑1 (MQ=255)                                                                                    cagggaaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATa                                                                                                                                                                                                                  <  1:238329‑M2/87‑1 (MQ=255)                                                                                         aaatgtgtgtcgtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCTGGGCGCATCTTACTGCGCAGATACGccc                                                                                                                                                                                                             <  1:149593‑M2/92‑1 (MQ=255)                                                                                                    gtgtaggtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAAtcca                                                                                                                                                                                                  >  1:59866‑M2/198‑299 (MQ=255)                                                                                                          gtcgaataaggcggtcgcgccgcatccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATa                                                                                                                                                                                            >  1:440397‑M2/192‑300 (MQ=255)                                                                                                                                  tccgacaatggccggcacatttgcctgatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCgag                                                                                                                                                                    >  1:557310‑M2/168‑300 (MQ=255)                                                                                                                                                            gatgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGcc                                                                                                                                          <  1:183277‑M2/159‑1 (MQ=255)                                                                                                                                                              tgcgacgctgacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGc                                                                                                                                        <  1:237551‑M2/161‑1 (MQ=255)                                                                                                                                                                       gacgcgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCttt                                                                                                                               >  1:58938‑M2/131‑300 (MQ=255)                                                                                                                                                                           cgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGcc                                                                                                                           <  1:59484‑M2/174‑1 (MQ=255)                                                                                                                                                                           cgtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGcc                                                                                                                           <  2:345988‑M2/174‑1 (MQ=255)                                                                                                                                                                           cgtcttatcaggcctaagtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGTGGGGCGCATCTTCCTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCGGCCGGCCGCTTAGTTAGCCGCTGACTCTTTTgca                                                                                                                           >  2:338211‑M2/127‑299 (MQ=255)                                                                                                                                                                            gtcttatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCAGAACGAAGTGCGCTACCAGGCTGCGCCAATCAACGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGTCGCTTAGTTAGCCGCTGCCTCTTTTGCCt                                                                                                                          >  2:134985‑M2/126‑300 (MQ=255)                                                                                                                                                                                tatcaggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGNGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTAGTCAATCCCTTAATAGCAAAATGTCTTTTGATCGGCGAGAAAGTCAGCAGGCAGCTTAGTTAGCCGCTGCCTCTTTTGCCTGcga                                                                                                                      >  2:158526‑M2/122‑299 (MQ=255)                                                                                                                                                                                     ggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGCACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGTATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTACTCGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCACTTAGTTAGCCGCTGCATCTTTTGCCTACGAGAtgt                                                                                                                 >  1:22068‑M2/117‑300 (MQ=255)                                                                                                                                                                                     ggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCCTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTGGCCGCTGCCTCTTTTGCCTGCGAGAtgt                                                                                                                 >  2:4235‑M2/117‑300 (MQ=255)                                                                                                                                                                                     ggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCCTAATAGCAAAATGCCTTTTGATCGGCGAGAAGGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGAtgt                                                                                                                 >  2:78051‑M2/117‑300 (MQ=255)                                                                                                                                                                                     ggcctacgtcgagtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgcaaaaaagcctgctcgttgagcaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTNACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGAtgt                                                                                                                 >  1:507733‑M2/117‑300 (MQ=255)                                                                                                                                                                                     ggcctaagtcgcgtcgaacctccctgcccccggaggttctcatcctcatgagctgcagatgtaaaaaagcctgctcgttgagtaggcttttcgaatttggtcggtgatagaggattCGAACCTCCGACCCCTTCGTCCAGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTAATGCGCAGATACGCCCTCGTCAATCCCTTAATAGCCAAATGTCTTTTGATCGGNGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCNAGAtgt                                                                                                                 >  2:574674‑M2/117‑300 (MQ=255)                                                                                                                                                                                                              gCCCCCGGAGGTTCTCATCCTCATGAGCTGCCGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTAGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAAGGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTNAGCCGCTGCCTCTTTTGCCTGCGAGGTGTGACACCAGTTGTTGTTTTNGTTAAt                                                                                        >  2:576195/1‑300 (MQ=255)                                                                                                                                                                                                              gCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAAt                                                                                        >  1:520935/1‑300 (MQ=255)                                                                                                                                                                                                                ccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTc                                                                                      >  1:514056/1‑300 (MQ=255)                                                                                                                                                                                                                    ggAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTccacc                                                                                  <  1:367824/300‑1 (MQ=255)                                                                                                                                                                                                                     gAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTccacca                                                                                 >  1:49245/1‑300 (MQ=255)                                                                                                                                                                                                                         ttCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGAAACCAGTTGTTGTTTTCGTTAATTCCACCATCCg                                                                             >  1:261216/1‑300 (MQ=255)                                                                                                                                                                                                                                 cTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGta                                                                     >  1:376890/1‑300 (MQ=255)                                                                                                                                                                                                                                           gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGTTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGAc                                                           <  2:371480/300‑1 (MQ=255)                                                                                                                                                                                                                                                      aaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGTTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCatgatg                                                <  1:271877/300‑1 (MQ=255)                                                                                                                                                                                                                                                      aaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCatgatg                                                <  2:114608/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGNGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTAc                                       <  1:508835/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTAc                                       >  1:436246/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  ctgcgagatgtgtataagagacagcGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGc                                    >  1:697295/25‑300 (MQ=255)                                                                                                                                                                                                                                                                         ggCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCTAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGt                             <  2:668304/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGt                             <  1:341537/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggCTTTGCGAATTTGGTCGTTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCTGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCTGGAAAGTCAGCAGGCTTCTTAGTTAGCCGCTGCCTCTTTTGCCTGCTAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGTTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGt                             <  2:238579/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ttttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTGAGCCACTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGAc                          <  2:42884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              ttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACgg                        <  1:211122/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ggTCGGTGGTAGAGGATTCGAACCTCCGACCCCTTCGTACCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTCTGNCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACt               >  1:467205/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       ggTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACt               >  1:110515/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           ggTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCa           <  1:384436/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCAt          >  1:221950/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gTGATAGAGGATTCAAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGAAACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCATACAGCTCTACGTGACGGCGTGGCACTTTCAt          >  1:225225/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    ggATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGctt  >  1:452784/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       CGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGGGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTT  >  NC_000913/3708339‑3708929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑