Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,164,339	2 bp→AT	coding (701‑702/1845 nt)	btuB →	cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,164,339	0	G	A	100.0%	119.2 / NA	34	W234Y (TGG→TAT)	btuB	cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/22);  total (12/22)
*	NC_000913	4,164,340	0	G	T	100.0%	114.8 / NA	34	W234Y (TGG→TAT)	btuB	cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/22);  total (12/22)

CGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATG  >  NC_000913/4164111‑4164577                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                              cggaaATTTCAGGAGGGAGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCggctat                                                                                                                                                                                                                          >  1:352302/1‑251 (MQ=255)    aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCggctatggc                                                                                                                                                                                                                       <  1:290326/251‑1 (MQ=255)         tCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGgctatggctatg                                                                                                                                                                                                                   <  2:28407/250‑1 (MQ=255)            ggagGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATa                                                                                                                                                                                                                >  1:105924/3‑250 (MQ=255)                              aGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGGCACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTGACTGATGCCTATAGCAGCTTTGTGCGCGGCTAAGGCTATGATAACCGTACCAATTATGAcgc                                                                                                                                                                                             <  2:163831/251‑1 (MQ=255)                                     aGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGGTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGtattat                                                                                                                                                                                      <  1:261674/251‑1 (MQ=255)                                       aaCTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATtc                                                                                                                                                                                    >  1:49236/1‑251 (MQ=255)                                         cTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCCTAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATtctc                                                                                                                                                                                  >  1:262699/1‑251 (MQ=255)                                           aggatgTCTCTACGCAGCAACAACTGGGGGATAAGACGCGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGGTATGATGTTTTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTGGTGCGCGGCTATGGCTAGGATAACCGTGCCAATTAGGACGCGTATTAGTCTcc                                                                                                                                                                                 <  2:231049/248‑1 (MQ=255)                                                       cGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTg                                                                                                                                                                    <  1:258322/251‑1 (MQ=255)                                                              acaacTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGAt                                                                                                                                                              >  1:82200/1‑250 (MQ=255)                                                                  cTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCg                                                                                                                                                         >  2:132927/1‑251 (MQ=255)                                                                                 cGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCa                                                                                                                                          <  1:288409/251‑1 (MQ=255)                                                                                  gggTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCaa                                                                                                                                         <  2:199588/251‑1 (MQ=255)                                                                                            gtttggCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACg                                                                                                                               <  2:107947/247‑1 (MQ=255)                                                                                            gTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACg                                                                                                                               <  1:12579/251‑1 (MQ=255)                                                                                                       aTGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGTCTgcg                                                                                                                     <  1:82739/250‑1 (MQ=255)                                                                                                           ccATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCtat                                                                                                                 >  2:195457/1‑250 (MQ=255)                                                                                                             aTACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAAc                                                                                                              <  2:367228/251‑1 (MQ=255)                                                                                                               aCTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACg                                                                                                             <  2:333715/250‑1 (MQ=255)                                                                                                                            atgTTGTTCCCTGTGGTTATAGCGGGAAGCACGCGCAAACCGATAACGCTGGTTTTTTAAGTAAACCGCTTTCTGGCGCGCTGGAGCATCACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGCTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCCAACTGATTaa                                                                                                >  2:25552/1‑250 (MQ=255)                                                                                                                              gttgttGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGAGTAAATc                                                                                             <  2:384134/251‑1 (MQ=255)                                                                                                                                tgttgCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATcac                                                                                           >  1:400340/1‑251 (MQ=255)                                                                                                                                     ccTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTc                                                                                      <  1:39321/251‑1 (MQ=255)                                                                                                                                             aaTACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAg                                                                              <  1:40689/251‑1 (MQ=255)                                                                                                                                                   ggAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAg                                                                        <  1:123265/251‑1 (MQ=255)                                                                                                                                                     aaCGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGcc                                                                      <  2:228362/251‑1 (MQ=255)                                                                                                                                                                      aTAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAAc                                                     >  1:387765/1‑251 (MQ=255)                                                                                                                                                                      aTAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAAc                                                     <  1:280446/251‑1 (MQ=255)                                                                                                                                                                          cGATGGTTCTTTAAGTAAAACGCTTTATGGAGCGCTTGAGCATAACATTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATAACCGTAAACTATATAGCCAAAGTTGGGACGCCGGGCTGCGCTATATCGGCGAAATGATTAAATCACAACTCATTACCAGCGATAGCCATAGCAATGATTACCACttcg                                                 <  2:6164/251‑4 (MQ=255)                                                                                                                                                                                 tttttAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATcccc                                           >  1:115918/1‑250 (MQ=255)                                                                                                                                                                                                                         ttACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCta                                                                           >  1:45222/1‑178 (MQ=255)                                                                                                                                                                                                                         ttACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCta                                                                           <  2:45222/178‑1 (MQ=255)                                                                                                                                                                                                                          tACTGATGCCTATAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAGCTCTATAGCCAAAGTTGGGACGCCGGGCTGCTCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAGTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagagg  <  2:275644/250‑3 (MQ=255)                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                              CGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATG  >  NC_000913/4164111‑4164577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑