Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,037,810	C→T	P24L (CCC→CTC)	appC →	cytochrome bd‑II subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,037,810	0	C	T	95.2%	138.4 / ‑5.7	42	P24L (CCC→CTC)	appC	cytochrome bd‑II subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/19);  minor base G (0/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTATGTCAGTGGCTGGCGGAAGCTGCACCGACGTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACCCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCCT  >  NC_000913/1037570‑1038049                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ggTATGTCAGTGGCTGGCGGAAGCTGCACCGACGTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTGTTTGTACTCCTTACCCTg                                                                                                                                                                                                                                       >  1:142021/1‑251 (MQ=255)        cAGTGGCTGGCGGAAGCTGCACCGACGTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTGCAGGATAAAGAGGGAGATCTAGCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCAGGTGGGATGTCATTGATTTATCGCGCTGGCAGTGTGCTCTGACCGCGCTGTATCACTTTTGATTTGTACGCCTTGCCCGGTGGCTg                                                                                                                                                                                                                                 <  2:244278/250‑1 (MQ=255)               tggcGGAAGCTGCACCGACGTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGAttttttt                                                                                                                                                                                                                         >  1:5186/1‑251 (MQ=255)                                  gTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAAcc                                                                                                                                                                                                      >  1:151976/1‑251 (MQ=255)                                  gTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAACCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTCTTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGCCCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGCAAcc                                                                                                                                                                                                      >  1:55811/1‑251 (MQ=255)                                          aCGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGt                                                                                                                                                                                              >  2:217935/1‑251 (MQ=255)                                               aaGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTGTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTc                                                                                                                                                                                          <  1:156478/250‑1 (MQ=255)                                                   aTCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTg                                                                                                                                                                                                                                       >  1:50299/1‑201 (MQ=255)                                                   aTCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCgg                                                                                                                                                                                     >  2:80294/1‑251 (MQ=255)                                                   aTCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCg                                                                                                                                                                                      <  1:409478/250‑1 (MQ=255)                                                   aTCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATAACTCTGGTCGTAGAGTTTCAGGAGAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTGCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGAACGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTg                                                                                                                                                                                                                                       <  2:50299/201‑1 (MQ=255)                                                            aGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACTGCGCTGTATGACTCTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGATATTATGGAAACCAGTTACGTGGTCCCCGGCAAAACAAt                                                                                                                                                                            <  2:322724/251‑1 (MQ=255)                                                                        atCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGatat                                                                                                                                                                >  2:413321/1‑251 (MQ=255)                                                                        atCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGatat                                                                                                                                                                <  1:164650/251‑1 (MQ=255)                                                                                 ggCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCtt                                                                                                                                                       <  2:174627/251‑1 (MQ=255)                                                                                   ggAATACCGCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCGCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCt                                                                                                                                                     <  2:241020/250‑1 (MQ=255)                                                                                    gAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTg                                                                                                                                                    <  2:275864/251‑1 (MQ=255)                                                                                     aaTACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTCTCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTgg                                                                                                                                                   >  1:406399/1‑251 (MQ=255)                                                                                      aTACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTc                                                                                                                                                                                          <  1:222696/211‑1 (MQ=255)                                                                                      aTACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTc                                                                                                                                                                                          >  2:222696/1‑211 (MQ=255)                                                                                                    agagTTTCAGGATAAAGAGGGAGATCTACCATTATCGTGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGGGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCgg                                                                                                                                     <  1:191300/250‑1 (MQ=255)                                                                                                                 aaaGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGct                                                                                                                        <  2:18611/250‑1 (MQ=255)                                                                                                                   agagGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGCGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCtt                                                                                                                     >  1:29207/1‑251 (MQ=255)                                                                                                                    gagGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTg                                                                                                                    >  1:130540/1‑251 (MQ=255)                                                                                                                            tCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCTCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGctc                                                                                                                       <  2:393082/240‑1 (MQ=255)                                                                                                                            tCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTCTTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGctc                                                                                                                       >  1:393082/1‑240 (MQ=255)                                                                                                                               aCCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTgg                                                                                                              <  2:130540/246‑1 (MQ=255)                                                                                                                               aCCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTgg                                                                                                              <  1:208999/246‑1 (MQ=255)                                                                                                                                ccATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCg                                                                                                        >  2:311831/1‑251 (MQ=255)                                                                                                                                          gTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCAt                                                                                              <  1:78351/251‑1 (MQ=255)                                                                                                                                               ttttCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGt                                                                                         >  1:21983/1‑251 (MQ=255)                                                                                                                                                              tACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACt                                                                           >  2:107582/1‑250 (MQ=255)                                                                                                                                                                 aGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACt                                                                       >  2:327918/1‑251 (MQ=255)                                                                                                                                                                                                             ttGCTCTGACCGCGCTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCAcc                            <  2:67230/250‑1 (MQ=255)                                                                                                                                                                                                              tGCTCTGACCGAGTTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCg                           <  2:319039/250‑1 (MQ=255)                                                                                                                                                                                                                          gcTGTATCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGa                >  1:200421/1‑249 (MQ=255)                                                                                                                                                                                                                               aTCACTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCGGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGcctt          >  2:327476/1‑247 (MQ=255)                                                                                                                                                                                                                                 cACTTGTTATTGGGACGCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGGGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAAt       <  1:328758/251‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGAt                   >  2:273863/1‑237 (MQ=255)                                                                                                                                                                                                                                   cTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGAt                   <  1:273863/237‑1 (MQ=255)                                                                                                                                                                                                                                   cTTTTTATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATgg     >  1:271541/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttATTTGTACTCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCct  <  2:174730/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                GGTATGTCAGTGGCTGGCGGAAGCTGCACCGACGTAAAAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGAGGGAGATCTACCATTATCGGGTTATTTTTCTCTCTTCGCCTACAGGAGTGCGCATGTGGGATGTCATTGATTTATCGCGCTGGCAGTTTGCTCTGACCGCGCTGTATCACTTTTTATTTGTACCCCTTACCCTGGGGCTGATTTTTTTGCTGGCTATTATGGAAACCATTTACGTGGTCACCGGCAAAACAATCTACCGCGATATGACGCGCTTCTGGGGTAAGCTCTTCGGTATCAATTTTGCTCTTGGCGTGGCTACCGGCCTGACCATGGAGTTTCAGTTTGGTACTAACTGGTCATTCTATTCCAACTATGTGGGCGATATTTTTGGCGCACCGCTGGCGATGGAAGCATTAATGGCCT  >  NC_000913/1037570‑1038049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑