Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,862	C→T	A31A (GCC→GCT)	hyaD →	putative hydrogenase 1 maturation protease HyaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,862	0	C	T	100.0%	128.0 / NA	38	A31A (GCC→GCT)	hyaD	putative hydrogenase 1 maturation protease HyaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/16);  total (22/16)

GGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCC  >  NC_000913/1035619‑1036097                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ggCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGACGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTAcc                                                                                                                                                                                                                                      >  2:397127/1‑251 (MQ=255)    tGGGGATGTGGCTGCTTGGCGAGTTTGTGATCGGTCATGTCTACATGGCGCTGCGCGCAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACt                                                                                                                                                                                                                                   >  2:381630/1‑251 (MQ=255)          tgtgGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGccc                                                                                                                                                                                                                              <  2:113614/250‑1 (MQ=255)                      gcgcGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGgaga                                                                                                                                                                                                                 <  1:100561/251‑1 (MQ=255)                            ttGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTc                                                                                                                                                                                                            <  2:335645/250‑1 (MQ=255)                            ttGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTc                                                                                                                                                                                                            <  1:202068/250‑1 (MQ=255)                              gtgATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGAt                                                                                                                                                                                                         >  1:251086/1‑251 (MQ=255)                               tgATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATg                                                                                                                                                                                                        >  1:207282/1‑251 (MQ=255)                                                       tGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATg                                                                                                                                                                                                        <  2:138090/227‑1 (MQ=255)                                                       tGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATg                                                                                                                                                                                                        >  1:138090/1‑227 (MQ=255)                                                        gCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCt                                                                                                                                                                               <  2:75278/251‑1 (MQ=255)                                                             aaGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTAACGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTggggg                                                                                                                                                                          >  2:243533/1‑250 (MQ=255)                                                                 catcatGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGt                                                                                                                                                                      >  1:319644/1‑251 (MQ=255)                                                                  atcatGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTc                                                                                                                                                                     >  1:184164/1‑251 (MQ=255)                                                                                           ccaccaTGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGtt                                                                                                                                                 <  2:251086/246‑1 (MQ=255)                                                                                                       aCGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGTCTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTg                                                                                                                                >  1:326946/1‑251 (MQ=255)                                                                                                            tACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTAc                                                                                                                           >  1:19480/1‑251 (MQ=255)                                                                                                                    ccACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGTGCAACGCGTGGTGGTCATGGGGCTGGGCACCCTGCTGCGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGCTGGCGGTACTCAGGGACTGACCTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctgg                                                                                                                    >  1:407455/1‑250 (MQ=255)                                                                                                                    ccACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCTGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGctgg                                                                                                                    >  1:409314/1‑250 (MQ=255)                                                                                                                                   aaTAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCg                                                                                                    <  1:328713/251‑1 (MQ=255)                                                                                                                                        gTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCt                                                                                               <  1:76056/251‑1 (MQ=255)                                                                                                                                         tAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCt                                                                                               >  2:186114/1‑250 (MQ=255)                                                                                                                                            cAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGc                                                                                           >  1:354928/1‑251 (MQ=255)                                                                                                                                             aaGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGcc                                                                                          >  2:92978/1‑251 (MQ=255)                                                                                                                                               ggatcGTTCATGAGCGAGCAACGCGTTGTGGTCCTGGTGCTGGGCAACCTGCTGTGGGCCGCTGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTCCCACTGGCCCGAGTATGTGGCGATTGTCGATGGCGGTACTCAGGGACTGAACTTTCTGGGGTATGTCTCAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTCCGGTCTGGCACCTTGCACGCTGCGACCCTATTCCgt                                                                                        >  2:66169/5‑250 (MQ=255)                                                                                                                                                 aGCGTTCCTGAGCGAGCAAAGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGGATGATCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGGTGATTCTCGATGCCATTGACTACGGGCTGGAACCGGGAACGCTGCGAACCTATGCCgg                                                                                        >  2:262828/1‑249 (MQ=255)                                                                                                                                                 aGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCgg                                                                                        >  1:223140/1‑249 (MQ=255)                                                                                                                                                           agcgagcAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTcc                                                                            <  1:308288/251‑1 (MQ=255)                                                                                                                                                                      cgTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTCTGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTAtctcc                                                                 >  2:73663/1‑250 (MQ=255)                                                                                                                                                                          gtggtCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAgcgc                                                             >  1:333506/1‑251 (MQ=255)                                                                                                                                                                                        gggCAACCTGCTGTGGTCCGATTAAGGCTTCGGCGTGCGGGTGGCGGAGCGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTTAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGAGTCTGTATGCCGTTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCAGTTCGTCTTAGCTCAGCCCGGAGAAAATGAGc                                                <  2:164390/250‑1 (MQ=255)                                                                                                                                                                                             aCCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCCGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCAt                                          <  2:207282/251‑1 (MQ=255)                                                                                                                                                                                                    gtgGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAAc                                    <  1:414143/250‑1 (MQ=255)                                                                                                                                                                                                                            gCGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGgcgttg            <  2:223140/250‑4 (MQ=255)                                                                                                                                                                                                                             cGGGTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTgttggcgttggc          <  1:231879/251‑1 (MQ=255)                                                                                                                                                                                                                              gggTGGCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGttggcgttggcg         >  2:311473/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggCGGAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGACGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGata     <  2:53709/251‑1 (MQ=255)                                                                                                                                                                                                                                     ggAACGGCTGTATGCTCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGTTGGCGGATATcc  >  1:316853/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            GGCTGGGGATGTGGCTGATTGGCGCGTTTGTGATCGGTCATGTCTACATGGCGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACCATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAAATAAGTAACAAGGAGCGTTCATGAGCGAGCAACGCGTGGTGGTCATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGAACGGCTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTACTCAGGGACTGAACTTGCTGGGGTATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCCGGAGAACGCATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCATCAGAACAGTTTCTCCGAAGTGTTGGCGCTGGCGGATATCC  >  NC_000913/1035619‑1036097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑