| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,018,193 | C→T | S36N (AGT→AAT) | ycbZ ← | putative ATP‑dependent protease YcbZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,018,193 | 0 | C | T | 96.5% | 97.0 / NA | 29 | S36N (AGT→AAT) | ycbZ | putative ATP‑dependent protease YcbZ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (13/15); minor base G (0/1); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGATTCTTTTCTTCACTGGCCTA > NC_000913/1017948‑1018437 | cAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATACGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAAt > 2:341515/1‑251 (MQ=255) gCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTCTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGa < 1:19481/251‑1 (MQ=255) atcTGCACTCACTGAGTCCCGTAAGCGGATGCAGTGGACGGCAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAAtgtg > 2:56465/1‑251 (MQ=255) cactcactGCGTTACGTAAGCGGATGGAGTGGCCGGAAACCTCAGAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCTGTTGAGTATCAGTGAATAAAGGATCGTTTTCGGCATTCAAATGTGTCTGa < 2:397422/251‑1 (MQ=255) actcactGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAg < 1:298932/251‑1 (MQ=255) ggCCGGAAACCTCATAGTGACCGCCCACCAGTTGGGCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATACGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATCAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGTTATc > 2:353510/1‑250 (MQ=255) ccTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGtt > 2:393794/1‑190 (MQ=255) ccTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCGTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACGGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGtt < 1:393794/190‑1 (MQ=255) ttGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGGCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCGATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGAt < 2:405789/250‑1 (MQ=255) tAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGttt > 1:156472/1‑251 (MQ=255) ggCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCaaa < 1:160636/250‑1 (MQ=255) aaGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGCGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCcgcg > 1:47011/1‑250 (MQ=255) aTTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGAtt > 1:56225/1‑250 (MQ=255) cAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAAcc < 1:311754/250‑1 (MQ=255) cAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAAcc < 1:311774/250‑1 (MQ=255) gACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGc > 2:270926/1‑251 (MQ=255) ggCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGt > 1:298755/1‑180 (MQ=255) ggCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGt < 2:298755/180‑1 (MQ=255) ccTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACg > 1:333583/1‑251 (MQ=255) gCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGGAACCGGCACTACAGCACACGTAGAAAGCa < 1:341804/251‑1 (MQ=255) gTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGc < 2:120170/251‑1 (MQ=255) gcagcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGtt > 1:124757/1‑250 (MQ=255) gcagcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGtt < 1:398298/250‑1 (MQ=255) agcaACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTaa > 1:249215/1‑250 (MQ=255) cAGCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGAttctttt < 2:163689/251‑1 (MQ=255) gCCGCGGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGAttcttttct < 1:61108/251‑1 (MQ=255) cgcgGTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATTGGCGTTAAAGCTCACAGGATGGAGATTCTTttcttc > 1:96241/1‑251 (MQ=255) gTTGAGTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCGGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGATTCTTTTCTTCACtt < 2:47011/251‑2 (MQ=255) gTATCATTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGATTCTTTTCTTCACTGGCCTa < 2:159203/251‑1 (MQ=255) | CAACTTGCGTTAAAGTCGCAAAATTATCATCTGCACTCACTGCGTGACGTAAGCGGATGGAGTGGCCGGAAACCTCATAGTGACCGCCCACCAGTTGGCCTGCATCGCTTTGTAGCGTACGCGCGGCATTGGCAATAAGATTCAGATACTCAGACTCTTCCGGGGCCTTCGCCAGCATAAAAGAGGAGGATGCTCGCGTATGCAGCAACTGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTACAGCACACGTAGAAAGCACCGACAATACTCCTGGCATGGGCGTTAAAGCTCACAGGATGGAGATTCTTTTCTTCACTGGCCTA > NC_000913/1017948‑1018437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |