Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,495	G→A	V143V (GTC→GTT)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,495	0	G	A	100.0%	166.0 / NA	45	V143V (GTC→GTT)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

TGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTC  >  NC_000913/400251‑400740                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      tGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCt                                                                                                                                                                                                                                                 >  1:275942/1‑251 (MQ=255)  gATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCtt                                                                                                                                                                                                                                                >  1:226902/1‑251 (MQ=255)  gATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCt                                                                                                                                                                                                                                                 <  2:154374/250‑1 (MQ=255)     cccTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGt                                                                                                                                                                                                                                             <  2:49879/251‑1 (MQ=255)                 gATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGgctg                                                                                                                                                                                                                                 >  1:323045/1‑251 (MQ=255)                 gATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGgctg                                                                                                                                                                                                                                 >  1:322014/1‑251 (MQ=255)                  aTCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGgctgc                                                                                                                                                                                                                                >  2:225987/1‑251 (MQ=255)                       tttATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGa                                                                                                                                                                                                                             >  2:387829/1‑249 (MQ=255)                              tcgaactcgaacGCCAGATCGCCGGCAATTGCGTACTGTTCTTCACTGGTAACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAAc                                                                                                                                                                                                                    >  2:381039/1‑251 (MQ=255)                                      gaacGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAAc                                                                                                                                                                                                            >  1:92600/1‑251 (MQ=255)                                            cAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAATAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACaa                                                                                                                                                                                                      <  1:398802/251‑1 (MQ=255)                                            cAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACa                                                                                                                                                                                                       >  1:306933/1‑250 (MQ=255)                                               aTCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTGTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGTATCGCGTAGCAGACGTCTGGTAACATCTTTGTCCATACAGGCTGCTGATGCCAGAACATCAGAACCTGCaagcg                                                                                                                                                                                                   <  2:125702/251‑4 (MQ=255)                                                      gCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAAtt                                                                                                                                                                                            >  1:81091/1‑251 (MQ=255)                                                       cAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTg                                                                                                                                                                                           <  2:224983/251‑1 (MQ=255)                                                                        tcACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTc                                                                                                                                                                           <  1:225987/250‑1 (MQ=255)                                                                               ttACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCa                                                                                                                                                                     >  1:188851/1‑249 (MQ=255)                                                                                 aCTTTGCTGACACCAACAGAAGAGCCATGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCACTGCAAgg                                                                                                                                                                  >  2:327619/1‑250 (MQ=255)                                                                                        tGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCGTCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACACGCAGCATTCCCTGCAAGGAACCATCt                                                                                                                                                          >  2:400884/1‑251 (MQ=255)                                                                                                aCAGAAGAGCCCTGATTAGCCGGTTTTAAAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAg                                                                                                                                                  <  2:156859/251‑1 (MQ=255)                                                                                                         cccTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTg                                                                                                                                         <  2:322014/251‑1 (MQ=255)                                                                                                                   ccGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCggg                                                                                                                               <  2:81091/251‑1 (MQ=255)                                                                                                                                           ccAGTTTAGACTCCAATTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCGACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAAc                                                                                                       <  1:277378/251‑1 (MQ=255)                                                                                                                                              gTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCGGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGc                                                                                                    <  1:237956/251‑1 (MQ=255)                                                                                                                                                     cTCCACTTCGGCAAAACTAATGTTGTGCCGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGt                                                                                             >  1:361463/1‑251 (MQ=255)                                                                                                                                                     cTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGt                                                                                             >  1:375061/1‑251 (MQ=255)                                                                                                                                                        cACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGtttt                                                                                          >  1:252314/1‑251 (MQ=255)                                                                                                                                                           ttCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTgcg                                                                                       >  1:60080/1‑251 (MQ=255)                                                                                                                                                                  aaaCTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAAtt                                                                                                                                                                                            >  1:343389/1‑143 (MQ=255)                                                                                                                                                                  aaaCTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAAtt                                                                                                                                                                                            <  2:343389/143‑1 (MQ=255)                                                                                                                                                                       aaTGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCt                                                                           <  1:103895/251‑1 (MQ=255)                                                                                                                                                                          gttgtGACGATTGGCGCGCGTAAGGGTAATAAATGGCGCAATGTTCAACCCGGCATCGCGTAGAAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCACCGGCTGACCGTTTTGCGCGTCGATAAGCTGAt                                                                        >  2:304630/1‑251 (MQ=255)                                                                                                                                                                          gttgtGACGATGGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGAt                                                                        <  2:306933/251‑1 (MQ=255)                                                                                                                                                                             gtgACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGc                                                                      <  1:387829/250‑1 (MQ=255)                                                                                                                                                                                                               gcAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGc                                   >  2:140006/1‑251 (MQ=255)                                                                                                                                                                                                                    gTTCAACCCGGCATCGCGAAGCAGACTTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACAGCCGCCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTTGCACCTGCGCAAGGCTGGGCGCCgtag                              <  2:365617/251‑4 (MQ=255)                                                                                                                                                                                                                      tCAGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAgg                             <  1:377363/250‑1 (MQ=255)                                                                                                                                                                                                                        aGCCCGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGgcg                          <  2:95208/251‑1 (MQ=255)                                                                                                                                                                                                                            cGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaac                      <  2:139559/251‑1 (MQ=255)                                                                                                                                                                                                                            cGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaac                      <  1:306650/251‑1 (MQ=255)                                                                                                                                                                                                                            cGGCATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGcgcaac                      <  1:190915/251‑1 (MQ=255)                                                                                                                                                                                                                               cATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAAc                                                                                                                                                                                                    >  1:9762/1‑74 (MQ=255)                                                                                                                                                                                                                               cATCGCGTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAAc                                                                                                                                                                                                    <  2:9762/74‑1 (MQ=255)                                                                                                                                                                                                                                    cgTAGCAGACGTTTGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATg              <  1:286857/251‑1 (MQ=255)                                                                                                                                                                                                                                                ttGGTAACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTc  >  1:43630/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      TGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACCTGCGCAAGGCTGGTCGCCGAAGGGCGCAACGCAATATGGGCAGGATCGTC  >  NC_000913/400251‑400740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑