Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	384,839	G→A	intergenic (‑223/‑393)	yaiS ← / → tauA	putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	384,839	0	G	A	100.0%	69.4 / NA	20	intergenic (‑223/‑393)	yaiS/tauA	putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

AAAACCTTATCCACTTAATGACACTCCATTTTATTTATTATACTACAAGCACAACGATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCACTAAAATGAAGTGTAGTAAT  >  NC_000913/384603‑385073                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           aaaaCCTTATCCACTTAATGACACTCCATTTTATTTATTATACTACAAGCACAACGATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGa                                                                                                                                                                                                                               <  1:4258/250‑1 (MQ=255)                          ccATTTTATTTATTATACTACAAGCACAACGATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAAGTAATTGCAttt                                                                                                                                                                                                      <  2:370480/250‑1 (MQ=255)                                          actacAAGCACAACGATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCtgt                                                                                                                                                                                      <  2:355424/250‑1 (MQ=255)                                                        gATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGttt                                                                                                                                                                       >  1:312208/1‑251 (MQ=255)                                                                  gagaCGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTa                                                                                                                                                             <  2:113544/251‑1 (MQ=255)                                                                            ctctGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGt                                                                                                                                                    >  2:115517/1‑250 (MQ=255)                                                                                         aGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGAt                                                                                                                                      >  2:336083/1‑251 (MQ=255)                                                                                             gTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTg                                                                                                                                    >  1:317280/1‑249 (MQ=255)                                                                                               tAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTAATTgaga                                                                                                                                 <  2:137924/250‑1 (MQ=255)                                                                                                                 tgttGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATAc                                                                                                              >  1:322840/1‑251 (MQ=255)                                                                                                                        aataaCTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCg                                                                                                        <  1:137163/250‑1 (MQ=255)                                                                                                                               gAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATataa                                                                                                 <  1:96568/250‑1 (MQ=255)                                                                                                                                         ggCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTttatta                                                                                      <  1:327396/251‑1 (MQ=255)                                                                                                                                                          gTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGCGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTattgatattgat                                                                     >  2:363388/1‑251 (MQ=255)                                                                                                                                                                cTGGGTAATGTTTCTATGCAATTCATATGTTAAGGGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAaat                                                               >  2:303972/1‑251 (MQ=255)                                                                                                                                                                 tGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAaat                                                               >  1:78006/1‑250 (MQ=255)                                                                                                                                                                            tCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGtaat                                                    >  1:110029/1‑250 (MQ=255)                                                                                                                                                                                                                           tATTTTGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCACTAAAATGAAGTgtagt     <  1:10838/250‑1 (MQ=255)                                                                                                                                                                                                                           tATTTTGTTTTATACATTATATTGAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAAGGTAATATGCGAGTGATGGATAATGATACAGTGATATAAACAATGCTAATATAATAGTTTTAATAGATGTATTGATATTGATAGAAATAATGAAGTAATATATCGCTTAATGTGGTTGTTTATGCATCACGAAAATGAAGTgtagt     <  1:414192/250‑1 (MQ=255)                                                                                                                                                                                                                              tttGTTTTATACATTATATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATAATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCACTAAAATGAAGTGTAGTAAt  <  2:280308/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                           AAAACCTTATCCACTTAATGACACTCCATTTTATTTATTATACTACAAGCACAACGATGCACTCAGAGACGTAATCTCTGGCGCAGCCAGATGTTAATTTATAAATGTTAAATGTTGCTAATAACTGAAAAGCAAGGGCTTTCAGACCCGTGGGTTCGACTGGGTAATGTTTCTATGCAATTCATATGTTAAGTGTTTGTATGTTTGGTATGTATAGTTATTTTGTTTTATACATTGTATTTAGATGTGATTTAGTTTGTCAATTAATTGCATTTAAAAAATATGTTCTGTGAACAAGCATTGTTTATATACATTATGTGAATGTAATATGCGAGTGATTGAGAATGATACAGTGATATATACAATGCGAATATAATAGTTTTATTATATGTATTGATATTGATAGAAATAATGAAGTAATAAATCTCGTAATGTGGTTGTTTATGCATCACTAAAATGAAGTGTAGTAAT  >  NC_000913/384603‑385073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑