Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	358,368	G→A	P108L (CCC→CTC)	cynR ←	DNA‑binding transcriptional dual regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	358,368	0	G	A	97.6%	133.9 / NA	41	P108L (CCC→CTC)	cynR	DNA‑binding transcriptional dual regulator CynR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (21/19);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGAC  >  NC_000913/358123‑358612                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tttCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGcc                                                                                                                                                                                                                                                  <  1:60537/250‑1 (MQ=255)     atcatGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATa                                                                                                                                                                                                                                              >  1:141827/1‑250 (MQ=255)        atGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGt                                                                                                                                                                                                                                          >  1:26439/1‑251 (MQ=255)              gCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCg                                                                                                                                                                                                                                    <  1:9651/251‑1 (MQ=255)                   tCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTaacg                                                                                                                                                                                                                               >  2:215171/1‑249 (MQ=255)                       cGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTgg                                                                                                                                                                                                                           >  1:322274/1‑251 (MQ=255)                                          gCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAg                                                                                                                                                                                                        <  1:23144/251‑1 (MQ=255)                                                gggtgatgTTGCGCCACGACTATCGCGACACTTTGTGTCAGTAAAGGCATTGGCTCCAGCGCCGGCGAGTGCACGGTCGCGAAGGCATTCCCAACGTCCAACTCGTCGCGGCAAAGCATTTCCTCGATTTTCGCCTGCGACATTTCCTTTAGCTTGAGCGGGGTGCGGGGATAGTGCGCTGAGAGACCCGCCATTAAGGTGCCTATAAAGTAGCTCGTAAAGGTGGGGGTTAGGGCGATACgggccgatat                                                                                                                                                                                                  <  2:247667/248‑10 (MQ=255)                                                             acACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGGAGCTCGTAAAGGTGGGGGTGCCGGCGATACGCAGCGATCCTCGCGTCAGCTCg                                                                                                                                                                                     >  2:381959/2‑251 (MQ=255)                                                                   cTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAAc                                                                                                                                                                                >  1:134623/1‑250 (MQ=255)                                                                        gCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATg                                                                                                                                                                          >  1:137982/1‑251 (MQ=255)                                                                                      cAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTAc                                                                                                                                                            >  2:371057/1‑251 (MQ=255)                                                                                      cAGTAAAGGAATTGCCCCCATCGCCGGCGAATGCACAGGCGCGAAGGCAATCCCCACGTCCAACTCGTCGCGGCAAAGCATATCCTCGAGTTTCTCCTGCGACATTTCCTGTAGCGGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTAc                                                                                                                                                            <  2:17826/251‑1 (MQ=255)                                                                                                aTTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGccccc                                                                                                                                                    >  2:393295/1‑249 (MQ=255)                                                                                                aTTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGCCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGACCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAg                                                                                                                                                  <  1:104239/251‑1 (MQ=255)                                                                                                     ccccAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCACCGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGAGGCTGGGATACCGCGCATAAAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTCCCCGCCCCCAGTTCCt                                                                                                                                             <  1:290726/249‑1 (MQ=255)                                                                                                      tCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTg                                                                                                                                            >  2:76382/1‑251 (MQ=255)                                                                                                      cccAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGAAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCGGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTg                                                                                                                                            <  2:141827/250‑1 (MQ=255)                                                                                                         aGCTCCTGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATACCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGCGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGCAGCTCGTAACGGTGGGGGCGACGGCGATCCGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTgca                                                                                                                                          >  2:170529/1‑248 (MQ=255)                                                                                                                         caGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGTCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTAGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTAc                                                                                                                         >  2:174149/1‑251 (MQ=255)                                                                                                                             cgcgAAGGCAATCCCACCGCCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGAGGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTc                                                                                                                     <  2:26439/251‑1 (MQ=255)                                                                                                                                          ccAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACACCCGGCTGGCGTACTGTCGCCAGACTTCTcc                                                                                                        >  2:88971/1‑251 (MQ=255)                                                                                                                                                   aacccggcgcgCCAAAGCATATCCTCGATTTACTCCTGCGACATTTCCTGTGTCTGGACCGTGATGCGGGGATAGCGCGCAAAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCTCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGt                                                                                               <  2:152508/244‑1 (MQ=255)                                                                                                                                                          cgcgGAAAAGCATATCCGCGTTTTTCTCCTGCGACTTTTCCTGTAGCGGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACg                                                                                         <  2:134623/250‑1 (MQ=255)                                                                                                                                                              gCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCg                                                                                    <  1:325729/251‑1 (MQ=255)                                                                                                                                                                             gATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGt                                                                     >  2:67700/1‑251 (MQ=255)                                                                                                                                                                                    tcCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAAc                                                                >  2:128601/1‑249 (MQ=255)                                                                                                                                                                                    cccTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAg                                                              <  1:67700/250‑1 (MQ=255)                                                                                                                                                                                          caCATTTCCTGGAGCTGGGGCGTGATGCTGGGATAGCGCGCAAAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCGCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTTCCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCAc                                                        <  2:64344/250‑1 (MQ=255)                                                                                                                                                                                             atgccctgaggctgggggggggtGCTGGGATAGAGCGAATAGAATTCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGAGCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGc                                                      <  2:401341/228‑1 (MQ=255)                                                                                                                                                                                                           gAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTa                                       <  1:390921/251‑1 (MQ=255)                                                                                                                                                                                                              cGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACt                                    >  2:120741/1‑251 (MQ=255)                                                                                                                                                                                                              cGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACt                                    <  1:388229/251‑1 (MQ=255)                                                                                                                                                                                                                 gATGCGGGGATAGCGCGAAAAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGc                                 <  2:368341/251‑1 (MQ=255)                                                                                                                                                                                                                       gggATAGCGCGCATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCCGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATct                           >  2:202386/1‑251 (MQ=255)                                                                                                                                                                                                                       gggATAGAGCGCATAGAAATCCGCCGTTAAGAGGCCGATCAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAAGCGCCCGTTTACCCGCCCCCAGTTCCTGTACCGCCCGGCTGGCGTACTGTCGCCAGACTTCTCATGCATCAGTGAGACGAATCGTTCGCCAGCTACGGTCACACAGCGGCAAGCCTAAACTCTCCTCTAACTGGCGAATct                           >  2:234525/1‑251 (MQ=255)                                                                                                                                                                                                                                cgcATAGAAATCCGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGaaa                  <  1:76382/251‑1 (MQ=255)                                                                                                                                                                                                                                    tAGAAATCCGCCATTAATAGGCCGATCAAGTAGCGCGTAAAGGGGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCACCATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGGAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTAGCCAGCGACGGTCAAACAGCGGCCCGCCTAACCTCTCCTCTACCTGGCGAATCTGCTGGGAAAgctc              >  2:163159/1‑249 (MQ=255)                                                                                                                                                                                                                                         cgccGCCATTAAGAGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCTCAGGtt         <  2:362408/249‑1 (MQ=255)                                                                                                                                                                                                                                            cGCCATTACGAGGCCGATAAAGTAGCTCGTAAACGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGAGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTACCTGGCGAATCTGGTGGGAAAGCGCAGGTTggg      >  2:25749/1‑251 (MQ=255)                                                                                                                                                                                                                                                aTTAAGAGGCCGATAAAGTAGATCGTAAAGGTGGGGGTGACTGCGATAAGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGCGACGACTCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGAc  >  2:212534/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TTTCATCATGCAAGCGACTCAACGCCACCTGTTCATGGACGGCCAGCGGATGATGTTGCGCCACGACTAACGCTAAACTTTCTGTCAGTAAAGGAATTGCCTCCAGCTCCGGCGAATGCACAGGCGCGAAGGCAATCCCAACGTCCAACTCGTCGCGGCAAAGCATATCCTCGATTTTCTCCTGCGACATTTCCTGTAGCTGGAGCGTGATGCTGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGAC  >  NC_000913/358123‑358612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑