Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	314,622	G→A	intergenic (‑1380/‑669)	ykgR ← / → insE1	putative membrane protein YkgR/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	314,622	0	G	A	100.0%	60.4 / NA	18	intergenic (‑1380/‑669)	ykgR/insE1	putative membrane protein YkgR/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/5);  total (13/5)

AAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTG  >  NC_000913/314381‑314865                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aaaCAACCACGATTTCGTTATTCAGTTCTGGCCCCCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTCTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCCGCCACGGCTGGGCATGGGAAATACTACGGTAACTGCTGATAATCACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAAAGATGCGac                                                                                                                                                                                                                                            >  1:366272/1‑251 (MQ=255)        cACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGAATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAAc                                                                                                                                                                                                                                     >  2:355517/1‑251 (MQ=255)                                    cTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACagctaa                                                                                                                                                                                                           >  2:23607/1‑249 (MQ=255)                                                               tCAGGTTCTTTTTCCACTCGCTGTCACCTTTGCCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTc                                                                                                                                                                               <  2:214467/250‑1 (MQ=255)                                                                cAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACTTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATTCTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGTAAGTACGGGGGTGGCTCg                                                                                                                                                                              <  2:255185/250‑1 (MQ=255)                                                                                 cGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGGAATATGGTaacaggg                                                                                                                                                            >  2:292345/1‑245 (MQ=255)                                                                                                                                                 aTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAgg                                                                                                                     <  2:336636/226‑1 (MQ=255)                                                                                                                                                 aTGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAgg                                                                                                                     >  1:336636/1‑226 (MQ=255)                                                                                                                                                      aaaTACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGAttt                                                                                       <  1:157248/251‑1 (MQ=255)                                                                                                                                                                        tgATAATAACGTGGAGAAAAATGTAGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCCAAATGAATGTAGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAat                                                                      >  1:238513/1‑250 (MQ=255)                                                                                                                                                                          ataatcACGTGGAGAAAAACGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACAGCGCGCGTCAAACTGGATGTCGATAAAGATTTCTCGCTGAAGGATTTTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGCCAAATAtg                                                                   >  2:273541/1‑251 (MQ=255)                                                                                                                                                                                aCGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTc                                                             <  1:23607/251‑1 (MQ=255)                                                                                                                                                                                           aaTGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGCTGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGc                                                  >  1:183646/1‑251 (MQ=255)                                                                                                                                                                                                cgcgTCGTTTGCCGCCAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTTTGATACGCCGACAAATATGTTGTTAACTCAGGGGGCAATAc                                             >  2:404859/1‑251 (MQ=255)                                                                                                                                                                                                  cgtcgtTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCCAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTTTTATACGCCGACAAATATGTTGTTCACTCAGGGGGCAATACAt                                           >  2:106601/1‑251 (MQ=255)                                                                                                                                                                                                                      gggACATTTTTAAGCAGTCAGCCAGATAACGAAGCGACAGGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGAGTCAAACTGAATGTCGATAAAGATTTCTCGATGAAGGATTCTTCGCTGGAAATGCTTTATACGATTTATAATACGCCGACAAATATGTTGTTCACTAAGGGGGCAATACATCGTACAGACGATCGTACTc                        >  1:246838/1‑250 (MQ=255)                                                                                                                                                                                                                                     aGTCAGCCAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCTGGAAATACAGGAGTGGCTCGGGCAAAATGGTACTGCGCGCGCCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATTCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGttt        >  1:261063/1‑251 (MQ=255)                                                                                                                                                                                                                                           ccAGATAACGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTtggctg  >  1:327507/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCATGGGAAATACTACGGTAACTGCTGATAATAACGTGGAGAAAAATGTCGCGTCGTTTGCCGCAAATGCCGGGACATTTTTAAGCAGTCAGCCAGATAGCGATGCGACACGTAACTTTATTACCGGAATGGCCACAGCTAAAGCTAACCAGGAAATACAGGAGTGGCTCGGGAAATATGGTACTGCGCGCGTCAAACTGAATGTCGATAAAGATTTCTCGCTGAAGGATTCTTCGCTGGAAATGCTTTATCCGATTTATGATACGCCGACAAATATGTTGTTCACTCAGGGGGCAATACATCGTACAGACGATCGTACTCAGTCAAATATTGGTTTTGGCTG  >  NC_000913/314381‑314865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑