Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,946	G→A	W386* (TGG→TGA)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,946	0	G	A	100.0%	218.4 / NA	61	W386* (TGG→TGA)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/31);  total (30/31)

AAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTG  >  NC_000913/287705‑288192                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       aaGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCGTCACCCAATCGACCGTGGCGCGCCGCGGACAGCACTTc                                                                                                                                                                                                                                               >  1:326228/1‑251 (MQ=255)              cgcgCAGCTGACCGGGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCGGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAg                                                                                                                                                                                                                                  <  2:358483/251‑1 (MQ=255)                             gAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGt                                                                                                                                                                                                                   >  1:94238/1‑251 (MQ=255)                                aGGCCCGCAATTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGCGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtct                                                                                                                                                                                                                <  1:235611/251‑1 (MQ=255)                                aGGCCCGCAAGTGGCCCGGCAGCCTGCAGGCGGTCCGGGCAACTGGCGGGGCGGTTTCGACCCCAGTTCGCCTGACCCCGGGGTGCAGACCCTTCCCATTCCGGTCCACGACCCCCCCGGCTCGCCCACCGCGCGGCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtct                                                                                                                                                                                                                <  2:319528/251‑1 (MQ=255)                                 ggCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTtctc                                                                                                                                                                                                               <  2:348577/251‑1 (MQ=255)                                  gCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCCCAATTTGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCg                                                                                                                                                                                                              <  2:201353/251‑1 (MQ=255)                                     cGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCg                                                                                                                                                                                                           >  1:169875/1‑251 (MQ=255)                                            aGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGAATCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACt                                                                                                                                                                                                    >  1:176630/1‑251 (MQ=255)                                                    agcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGACTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagcag                                                                                                                                                                                            <  2:72281/251‑1 (MQ=255)                                                     gcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGGCACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACCTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCcagccgg                                                                                                                                                                                           >  1:287853/1‑248 (MQ=255)                                                     gcCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCaga                                                                                                                                                                                           <  1:148262/251‑1 (MQ=255)                                                          cAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGcgcg                                                                                                                                                                                      <  1:310057/251‑1 (MQ=255)                                                           aGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCgg                                                                                                                                                                                     <  2:143913/251‑1 (MQ=255)                                                            gCCGGTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCggg                                                                                                                                                                                    <  2:221094/251‑1 (MQ=255)                                                              cGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCCGAAACGCGGAAGTAGTTCTTGCCGGTGCACATCCAGCAGAGCGCGGGGc                                                                                                                                                                                  >  2:337090/1‑251 (MQ=255)                                                              cGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGc                                                                                                                                                                                  <  2:312002/251‑1 (MQ=255)                                                                     ggCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTg                                                                                                                                                                           <  1:89369/251‑1 (MQ=255)                                                                            ggCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGctact                                                                                                                                                                      <  2:214948/249‑1 (MQ=255)                                                                                ggACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTacaac                                                                                                                                                                 >  2:109243/1‑250 (MQ=255)                                                                                        tCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCGGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaaaaa                                                                                                                                                         <  2:369284/250‑1 (MQ=255)                                                                                             gCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTgg                                                                                                                                                     >  1:37101/1‑249 (MQ=255)                                                                                                   tCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACt                                                                                                                                              <  1:378603/250‑1 (MQ=255)                                                                                                     gCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGTCCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCt                                                                                                                                           >  2:279907/1‑251 (MQ=255)                                                                                                     gCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCt                                                                                                                                           >  1:324940/1‑251 (MQ=255)                                                                                                             cGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc                                                                                                                                   >  1:161875/1‑251 (MQ=255)                                                                                                                ggttGCAGACCCTTCGCATTTCGTTCGACGACACCCGCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACTGCTCTATGGCAACGACTCGCGCAATTCGACCTTCGCCCAATCGACCGTGGCGCGCCGCTGACGGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGTCGGTGCACTTCCAGCAGAGCGCGGGGCGGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACtgc                                                                                                                                <  2:339970/248‑3 (MQ=255)                                                                                                                      gggcccTGCGCATTCCGTTCGAGGCCCCCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAGCGACTCGCTCAATTCGACCTTCCCCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgaggag                                                                                                                          <  2:373833/248‑1 (MQ=255)                                                                                                                      aGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTCCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCCCCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCCGAAACGCGGCTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgagg                                                                                                                            >  2:380390/1‑249 (MQ=255)                                                                                                                         cccTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAggg                                                                                                                        >  1:74408/1‑250 (MQ=255)                                                                                                                           cTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCACCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACCGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGAc                                                                                                                      >  2:99164/1‑250 (MQ=255)                                                                                                                                  ttCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGt                                                                                                                 >  2:71950/1‑248 (MQ=255)                                                                                                                                    ccGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAc                                                                                                            >  1:180022/1‑251 (MQ=255)                                                                                                                                      gTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAcc                                                                                                           >  1:170895/1‑250 (MQ=255)                                                                                                                                          gacgacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGTTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCaa                                                                                                      <  2:204634/251‑1 (MQ=255)                                                                                                                                               cacCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa                                                                                                                                    <  2:142174/216‑1 (MQ=255)                                                                                                                                               cacCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGa                                                                                                                                    >  1:142174/1‑216 (MQ=255)                                                                                                                                                 cccTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCCATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGAAGAACCATCAAAGTTATc                                                                                               >  2:318282/1‑251 (MQ=255)                                                                                                                                                   cTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATcc                                                                                              >  2:189847/1‑250 (MQ=255)                                                                                                                                                   cTCGGCTCGCTCACCGCGCGCCCGGGCTTCCTCCGGCTCTATGGCAACCACTCCCTCAATTCGACCTTCACCCAATCGACCGAGGCGCGCCGCTGACAGCACTTCGCCCTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAAACATCAAAGGTAtacc                                                                                             >  2:189834/1‑248 (MQ=255)                                                                                                                                                      ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCCCCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCGCTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCt                                                                                          >  2:74291/1‑251 (MQ=255)                                                                                                                                                      ggCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATAGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCt                                                                                          >  2:133366/1‑251 (MQ=255)                                                                                                                                                          cgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgaggag                                                                                                                          <  1:71204/215‑1 (MQ=255)                                                                                                                                                          cgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACgaggag                                                                                                                          >  2:71204/1‑215 (MQ=255)                                                                                                                                                               cccGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAATAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACaa                                                                                 <  2:163108/250‑1 (MQ=255)                                                                                                                                                               acagcgcgcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCACAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAACGTTATCCAGCTCGACcaca                                                                                  >  2:230039/4‑250 (MQ=255)                                                                                                                                                                     cgcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCGCAACGTGcc                                                                           <  1:130663/251‑1 (MQ=255)                                                                                                                                                                     cgcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGACAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGcc                                                                           <  1:44571/251‑1 (MQ=255)                                                                                                                                                                      gcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCcg                                                                          >  1:256887/1‑251 (MQ=255)                                                                                                                                                                                   aCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTg                                                              <  1:2135/250‑1 (MQ=255)                                                                                                                                                                                                aaCGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCAt                                                <  2:169875/251‑1 (MQ=255)                                                                                                                                                                                                    aCTCGCTCAATTCGCCCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCTCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACGGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGGAGCCCATTCCg                                            <  2:371249/251‑1 (MQ=255)                                                                                                                                                                                                               tCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAAc                                  >  2:128032/1‑250 (MQ=255)                                                                                                                                                                                                                        aCCCAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGagag                        <  2:141494/251‑1 (MQ=255)                                                                                                                                                                                                                           cAATCGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGt                     >  1:376416/1‑251 (MQ=255)                                                                                                                                                                                                                              gcGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTg                  <  2:74408/250‑1 (MQ=255)                                                                                                                                                                                                                               cGACCGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTgg                 >  1:28981/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGc             <  1:215661/251‑1 (MQ=255)                                                                                                                                                                                                                                    gTGGCGCGCCGCTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCg            <  2:94238/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccgcTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACg    >  2:221695/1‑251 (MQ=255)                                                                                                                                                                                                                                              gcTGACAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTg  <  1:291496/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       AAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGCCTGCAGCCGTTCCGGGCAACTGGCGGGACGATTTCGACGCCAGTTCGCTTGACCCGGAGCTGCAGACCCTGCGCATTCCGTTCGACGACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAGGGTAGAACCATCAAAGTTATCCAGCTCGACCACAACGTGCCGTCGTGGCCGCTGCACGAGCAGCCCATTCCGGTGCCGGAACATGCGGAGAGCGTCTGGCTGCGGGTGGACGTG  >  NC_000913/287705‑288192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑