Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,324	G→A	G179D (GGC→GAC)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,324	0	G	A	96.2%	151.7 / NA	52	G179D (GGC→GAC)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (19/31);  minor base C (0/2);  total (19/33)
Fisher's exact test for biased strand distribution p-value = 5.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGA  >  NC_000913/287085‑287561                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              aaccGGCCGCAACTTCCTCGTCGCCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGctct                                                                                                                                                                                                                                    <  2:188546/248‑1 (MQ=255)  aaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGctc                                                                                                                                                                                                                                     <  1:33950/249‑1 (MQ=255)      cccGCAACTCCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTCCCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTac                                                                                                                                                                                                                               <  2:84127/250‑1 (MQ=255)         gaaaCTTCCTCGGCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACAcc                                                                                                                                                                                                                            <  1:328753/249‑1 (MQ=255)            acgtCCTCGGCACCGCGCCCCCAATCGAGGGGCCAGGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGCCCCGCCCCTTTTCCCCGACGACGATGGCCGCAAATACTAGATCTACCGCCCGTGGGGGCCGCGCCACCACAGCACCCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGaa                                                                                                                                                                                                                         <  2:353705/248‑1 (MQ=255)               tCCTCGTCACGGCGCCCTCCATCGAGGGGCCAGGGGGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCGGTTTACCGACACGCCGCTCTGCTACACCGAAga                                                                                                                                                                                                                       <  2:339588/250‑2 (MQ=255)               tCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCGCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAga                                                                                                                                                                                                                       <  1:208782/250‑2 (MQ=255)                            gcCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATc                                                                                                                                                                                                         <  1:57505/251‑1 (MQ=255)                                           gCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACAcgcc                                                                                                                                                                                                                                         <  1:152694/204‑1 (MQ=255)                                            ccATGGACCGAGCCAAGCCCGATGGGCACCGGCGGGTTTGACCCGTCCCGGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCCCAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGta                                                                                                                                                                                         <  2:177807/251‑1 (MQ=255)                                               tGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGta                                                                                                                                                                                         <  1:76174/248‑1 (MQ=255)                                               tGGAGCGAGCCAATCCCGATGGGCAACGGCGGGGTTGACCCGTCCCCGTTCCACGACGACGATGGGCGCAAATACTATATCTACCGCCCGCGGGGGGCGCGCCCCCCCACCAACCCCCCCCACACCATCGTGTTACAGGCGGTTGACCCCCAGACCGGCACGCCCCCCCCCCCGCGCAAAAAGCTGTTTTCCGACACCCCCCTCCGCTACACCCAAGACGCGCCCCCGTATCGccccccggggtgggg                                                                                                                                                                                         >  2:76174/1‑235 (MQ=255)                                                        ccAATCCCGATGGGCAACGGCGGGTTTGACCCCTCCCCGTTTCACGACGACGATGGCCGCAATTACTATATTTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCCCCCAACACCATCGGGTTACAGGCGGTTGACCCCCAGACCCGCACACCCTCGCCCCAGCGCAAAAAGCTGTTTACCCACACGCCCCTCTGCTCCACCGAAGACGCGCACCCGGATCGCCCCCCGGGAGGGGACCACCTGATggg                                                                                                                                                                             >  1:348413/1‑250 (MQ=255)                                                                       aaCGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCAc                                                                                                                                                              <  1:13999/251‑1 (MQ=255)                                                                        aCGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTCCCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACCCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCAcc                                                                                                                                                             <  2:343731/251‑1 (MQ=255)                                                                             gggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCCCCAGCtc                                                                                                                                                        >  2:86833/1‑250 (MQ=255)                                                                              ggTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTAc                                                                                                                                                       >  1:79372/1‑251 (MQ=255)                                                                                              gTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtcgt                                                                                                                                         <  1:160460/249‑1 (MQ=255)                                                                                                       cgacgaTGGCCGCAAATACTATATCTCCCGCCCGGGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGtt                                                                                                                              <  2:42385/251‑1 (MQ=255)                                                                                                        gacgaTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTc                                                                                                                             <  1:271134/251‑1 (MQ=255)                                                                                                           gaTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGGACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGGCGTGGTGCTGCGTTCCa                                                                                                                           >  2:158356/1‑250 (MQ=255)                                                                                                            aTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCaa                                                                                                                          >  1:331242/1‑250 (MQ=255)                                                                                                            aTGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCaa                                                                                                                          >  1:220552/1‑250 (MQ=255)                                                                                                             tGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGCTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGCGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGCTCCaca                                                                                                                         >  2:286875/1‑248 (MQ=255)                                                                                                                     aatccattatCTACCGCCCGTGGGGGCCGCGCCACCACAGACACCCGCACAACCCCATCTTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTTCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCTAGGGCGGCACCAGCTACGATCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAc                                                                                                                <  1:246203/244‑1 (MQ=255)                                                                                                                      aTACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACg                                                                                                               <  1:760/251‑1 (MQ=255)                                                                                                                           atatCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGcc                                                                                                           <  1:213741/250‑1 (MQ=255)                                                                                                                           atatCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCg                                                                                                          >  1:52298/1‑251 (MQ=255)                                                                                                                             atCTCCCGCCCTGGGGGGCCGCGCCCCCACAGCACCCCGCCCAACACCATCGTTTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCCCCCGAGCGCAAAACGCTGTTTACCGACCCGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGt                                                                                                         <  2:148409/250‑1 (MQ=255)                                                                                                                                 cccGCCCGTGTGGGCCGCGCCCCCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAg                                                                                                    <  2:327392/250‑1 (MQ=255)                                                                                                                                       cGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCAc                                                                                              >  1:130974/1‑251 (MQ=255)                                                                                                                                        gTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCAcc                                                                                             >  2:332456/1‑251 (MQ=255)                                                                                                                                          gggggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCg                                                                                           >  1:93554/1‑251 (MQ=255)                                                                                                                                             ggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGAc                                                                                        <  1:332456/251‑1 (MQ=255)                                                                                                                                             ggCCGAGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCGCCGTTCCAAAAATATCGACGGGCCCTACGCGCTGCACCCggcc                                                                                        >  2:394746/1‑249 (MQ=255)                                                                                                                                                cgcgCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGt                                                                                      >  2:46043/1‑250 (MQ=255)                                                                                                                                                cgcgCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAAACCGCCACGCTCCCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCCCGGCTCCACGGAGGACGCCCACTTGAATCGCACCGCGGGAGGGAACTCCCGAATGCCCGCCGAGGGCGGCACCAGCTCCGGGCACGCCGCCGGGGGGCTGGGTCCCAAAAAAACCGACGGGCCgaccgggcggcccccggacga                                                                                      >  2:256313/1‑230 (MQ=255)                                                                                                                                                  cgcCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAAc                                                                                   <  2:41997/251‑1 (MQ=255)                                                                                                                                                             cAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCa                                                                        <  1:200828/251‑1 (MQ=255)                                                                                                                                                              aaCCCGCACAACCCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACAGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATCTCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGAcc                                                                         >  1:339970/1‑249 (MQ=255)                                                                                                                                                               ccccGCACAACACCACCGTGTTACAGGCGTTTGCCCCGCAGACCGGCACGCCCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCCCCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGc                                                                      <  1:237225/250‑1 (MQ=255)                                                                                                                                                                        acacCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTg                                                             <  1:276142/251‑1 (MQ=255)                                                                                                                                                                          cccATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAACCGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGcc                                                           <  2:90580/250‑1 (MQ=255)                                                                                                                                                                              ccGTGTTACAGGCGTTTGCCCCGCAGACCGGCACGCTCTCGCCCGGGCGAAAACCGCTGTTTCCCGCCACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCTGGACGTAACGATGATGACCAGCTGGCGCCTGCCGgag                                                       <  2:171856/250‑1 (MQ=255)                                                                                                                                                                                                      aGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGACGACGCGCACCTGTCTCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTCCGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGCGCTGCACCCGGACGTAACGATGATGAccc                                                                        >  1:395472/1‑209 (MQ=255)                                                                                                                                                                                                           ggCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTc                          >  1:93449/1‑251 (MQ=255)                                                                                                                                                                                                                     cGCCCGAGCGCAAAACGCTGTTTCCCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCtgctgc                  <  1:79214/249‑1 (MQ=255)                                                                                                                                                                                                                       cccGGGCGCAAACCGCTGTTTACCGCCACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGAc              <  2:138801/251‑1 (MQ=255)                                                                                                                                                                                                                        ccGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACg             >  1:289991/1‑251 (MQ=255)                                                                                                                                                                                                                             cgcAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATAc        <  1:411458/251‑1 (MQ=255)                                                                                                                                                                                                                             cgcAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATAc        <  1:236469/251‑1 (MQ=255)                                                                                                                                                                                                                                   aCGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGATGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGa  <  2:362048/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              AAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGA  >  NC_000913/287085‑287561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑