Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	238,884	G→A	intergenic (‑148/+535)	ykfM ← / ← yafV	uncharacterized protein YkfM/2‑oxoglutaramate amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	238,884	0	G	A	100.0%	58.9 / NA	18	intergenic (‑148/+535)	ykfM/yafV	uncharacterized protein YkfM/2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

ACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCGATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGA  >  NC_000913/238639‑239121                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              aCTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTc                                                                                                                                                                                                                                          <  1:215551/251‑1 (MQ=255)           aaaaCAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAaaca                                                                                                                                                                                                                                <  1:203842/251‑1 (MQ=255)             aaCAAAGAAGCATTGAATGCAGGGAAAAATAATATAGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTAATCTCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATAAAGTTCCTGTTGCCGTTTGAGTACATCTCTCATTGAATATTTTAATTTTTCTGATAAATGACATTGAGCATCAATTTCATTTAAaacaa                                                                                                                                                                                                                               >  2:351015/1‑250 (MQ=255)                aaaGCAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAAAGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCTGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGt                                                                                                                                                                                                                           >  2:369902/1‑251 (MQ=255)                     agCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTacaca                                                                                                                                                                                                                      <  2:379125/251‑1 (MQ=255)                       cATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACt                                                                                                                                                                                                                    >  2:75565/1‑251 (MQ=255)                                cAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTc                                                                                                                                                                                                           <  1:146291/251‑1 (MQ=255)                                                       aaaaaCATCGAAAGAAACTCTTTTAATTTAACATGTAAGCGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAg                                                                                                                                                                                    <  1:238057/251‑1 (MQ=255)                                                                        ctctTTTAATTTAACATGTAAACGCATGGTTAATCCTCAAATCACGCGTGGCGCCATAAGAACATACATAAATACCGTCATGTTTTCCCTTTTCCATTTATCACGTTCCTGCTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTACTCCGACAAGAATTACTGCAAAGCCa                                                                                                                                                                    >  1:52890/1‑250 (MQ=255)                                                                                  ttaaCATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAacac                                                                                                                                                          <  2:158814/250‑1 (MQ=255)                                                                                                       aatacTCAGATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTATTAGTCCATCACTAATTGCATATTTTAATTATTCTGATAAAAGGCATTGAGCATCAATTTCATGTAAAACAACGGTACACACTATCCCTGACAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAATGAAGCCAGACTTct                                                                                                                                    <  2:98119/247‑2 (MQ=255)                                                                                                        aTCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTcc                                                                                                                                    >  2:294591/1‑250 (MQ=255)                                                                                                                                             aaaTGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTg                                                                                               <  2:142832/250‑1 (MQ=255)                                                                                                                                                                                            gTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGctct                                               >  2:338337/1‑251 (MQ=255)                                                                                                                                                                                                  gTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTc                                         >  1:50087/1‑251 (MQ=255)                                                                                                                                                                                                                        tAATTTTTCTGATAAATGGCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTctgc                   >  2:46064/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaTGGCATTGAGCATCAATTTCATTTAACACACCTGTACACACTATCCCTGTCAGTAAAATTGCTCATCCGACACAAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGc       >  2:202106/1‑250 (MQ=255)                                                                                                                                                                                                                                          gCATTGAGCATCAATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAgaga  >  2:60879/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              ACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTCCCTTTTCCATTTATCAAGTTCCTGTTGCCGTTTTAGTCCATCTCTAATTGCATATTTTAATTTTTCTGATAAATGGCATTGAGCATCGATTTCATTTAAAACAACTGTACACACTATCCCTGTCAGTAAAATTGCTCCTCCGACAAGAATTACTGGAAAGCCAAAGCTAACACAGGCAAAAGAAGCCAGACTTCCAATAGCGGTTGCAACACCACCGGCTATAACTGCTTTGGCCACATCCATTGAAAAATCGCCAAGAAAATTAACCAGATCTCGCTCTGAACTCATAATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGA  >  NC_000913/238639‑239121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑