Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	126.4 / NA	37	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/28);  total (9/28)

GTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTC  >  NC_000913/4223394‑4223876                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ggtCGCCAACCTTACGCACGAACCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTc                                                                                                                                                                                                                                             <  2:366013/249‑1 (MQ=255) gTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTc                                                                                                                                                                                                                                             <  2:97493/251‑1 (MQ=255)     gCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTa                                                                                                                                                                                                                                         <  2:229478/251‑1 (MQ=255)        aaCCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCg                                                                                                                                                                                                                                      <  1:223641/251‑1 (MQ=255)                     aaaCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGGCCGC‑ATTGGCGGGAATGGGGGCGACCATAAC‑AGCCTCCTTTTTTTGTTTCGTTGGAAtaatttt                                                                                                                                                                                                                         >  1:260714/1‑251 (MQ=255)                       aCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAAtcattttca                                                                                                                                                                                                                       <  1:244270/251‑1 (MQ=255)                                      cGTGGTTAGCAGGCGGGGGGTGGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAGCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTGTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGGCCATGAC‑AGTCTCCTTGTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTGAGCt                                                                                                                                                                                                        <  2:22010/251‑1 (MQ=255)                                         ggTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTGCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa                                                                                                                                                                                                      <  2:209918/250‑1 (MQ=255)                                          gTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATg                                                                                                                                                                                                    <  2:377216/251‑1 (MQ=255)                                                 ggCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTG‑CCGCGTTTCGCGGGGATGGGGGGGACCCAGAC‑AGTCTCCTTTTTTTGTATTGTGGGAATTCTTTTCTTTTTTTTTTTTTGCTTATGCAATAGt                                                                                                                                                                                             >  1:34688/1‑251 (MQ=255)                                                   cGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTa                                                                                                                                                                                           <  1:285173/251‑1 (MQ=255)                                                            gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTT‑TCGCGTTTTGCGGGGATGGGGGGGGCCCAGAC‑AGGCTCCTTTTTTTTTTTCGTGGGAATCTTTTTCTTTTTTTTTTTTTGCTAATGccattatttatgaaatg                                                                                                                                                                                   >  2:232760/1‑234 (MQ=255)                                                            gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGAGTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAAGCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactga                                                                                                                                                                                  <  1:342389/251‑1 (MQ=255)                                                                   tGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGAt                                                                                                                                                                            <  2:413938/250‑1 (MQ=255)                                                                             ggCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGtt                                                                                                                                                                 <  1:95000/251‑1 (MQ=255)                                                                                  gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTGACTGAACTGATCCGATGAGTTAATGTTGAAc                                                                                                                                                             <  1:380684/250‑1 (MQ=255)                                                                                     gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAt                                                                                                                                                         <  2:374083/251‑1 (MQ=255)                                                                                                      gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTT‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑CGTCTCCTTTTTCTTTATCTTGGGAATCATTTTTCTTTTTTTTTTTTGCTAATGCAATAGATACTGAACTGATCCGATGAGTTAATGTTTTACAAATCTCATGTT‑GCG‑tggtgg                                                                                                                                      >  1:221991/1‑251 (MQ=255)                                                                                                              gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCtt                                                                                                                                <  1:232760/249‑1 (MQ=255)                                                                                                                    ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACcaca                                                                                                                        <  2:176037/251‑1 (MQ=255)                                                                                                                      aTTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTG‑CCGCGTTTCCCGGGGAGGGGTGGGACCATGAC‑AGTGTCCTTTTTTTGTATCGTGGGAATCATTTTTCTTTTTTTTTTTAGCTAATGCACTAGTTACTGAACTGATTCGGAGAGGTAATTGTGTACAAAACTCATGTT‑TCG‑TGGGGGGCGTTTTTAcacc                                                                                                                         >  2:106234/1‑245 (MQ=255)                                                                                                                             aaTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGttt                                                                                                              <  2:11534/251‑1 (MQ=255)                                                                                                                                ccACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTAt                                                                                                            <  1:319416/250‑1 (MQ=255)                                                                                                                                       aGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGGCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTAt                                                                                                    <  1:366623/251‑1 (MQ=255)                                                                                                                                         tAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTAGCAACGGCGGGATTTTTG‑CCCCGCTTCGCGGGAATGGGGGCGAACATGAA‑AGTCTCCTTTTTCTGTATCGGGGGAATCATTTTTCTTTTTATTGTTTGCTACTGCAATAGTTAATGAACTGATCCCATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATgg                                                                                                  >  1:402413/1‑251 (MQ=255)                                                                                                                                          aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTGCCACAGA‑TGCGTTTATGCCAGTATgg                                                                                                  <  1:124748/250‑1 (MQ=255)                                                                                                                                                 ggCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGATCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGa                                                                                          <  1:207479/251‑1 (MQ=255)                                                                                                                                                     aCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACGGAACTGATCCGATGAGTTAATGTTGAACAAATCTCAGGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TTCGTTTATGCTAGTATGGTTTGTTTAAttt                                                                                      <  2:198965/251‑1 (MQ=255)                                                                                                                                                      cgcgTTAAAGACTGATCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTGTCTG‑CCGCGTTTCGCGTGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCg                                                                                                                                                                                                                                      <  1:402674/109‑1 (MQ=255)                                                                                                                                                       gcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAAttttt                                                                                    <  1:201696/251‑1 (MQ=255)                                                                                                                                                              aGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTC‑CCGCGCTTCGCGGGGATGGGGGGGGACATGGC‑CGACTCCTTTTTTTTTATCGGGGGAATCATTTTTTTTTTTTTTTTTTGATCATTCAATAGTTTCTGAACAGATCCCATTAGTTTATGTTTAAAAAATCTCATGTT‑GCGGTGG‑GGGGGCTTTTTCCACAGA‑TGCGTTTTTGCCAGTTTGGGTTGTTTAAtttttttttaat                                                                             >  2:249170/1‑244 (MQ=255)                                                                                                                                                              aGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTG‑CCCCGTTTCCCGGGGATGGGGGGGACCAAGACCAG‑CTCCTTTTTTTGTATCCTGGGAATCATTTTTTTTTTTTTTGTTTTCTCATGCAAATGTTTATGCACTGATTCCATGAGGTTATGTTTGACAAAACTCATGTTTGC‑‑TGGTGGTCGCTTTTTCCACAGAATAGGTTT‑TGCCCGTATTGTTTGTTGGAtttttttttaat                                                                             >  2:151123/1‑244 (MQ=255)                                                                                                                                                                      ccTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTg                                                                     <  2:135763/251‑1 (MQ=255)                                                                                                                                                                                                                  gcgTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgc                          <  2:34688/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa         <  2:260714/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTGCGACCATGAC‑AGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGaa         <  1:360698/251‑1 (MQ=255)                                                                                                                                                                                                                                          cATGAC‑AGTATCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTc  >  1:323113/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTG‑CCGCGTTTCGCGGGAATGGGTGCGACCATGAC‑AGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTT‑GCG‑TGGTGGTCGCTTTTACCACAGA‑TGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTC  >  NC_000913/4223394‑4223876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑