Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,164,123	C→G	A162G (GCA→GGA)	btuB →	cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,164,123	0	C	G	100.0%	190.7 / NA	52	A162G (GCA→GGA)	btuB	cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/29);  total (23/29)

AGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATG  >  NC_000913/4163878‑4164368                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGt                                                                                                                                                                                                                                                  <  1:278648/251‑1 (MQ=255)  ggTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCCCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAggggg                                                                                                                                                                                                                                                 >  2:39321/1‑249 (MQ=255)  ggTCAGCTCTCATCTATTTTTATTCGCGGGACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                                                                                                                                                                                 <  2:108080/251‑1 (MQ=255)    tCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                                                                                                                                                                               >  1:333715/1‑251 (MQ=255)       gCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGaa                                                                                                                                                                                                                                            <  2:127524/251‑1 (MQ=255)         tctcATCTATTTTTATTCGCGGTACCAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTCCGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCCCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAgggg                                                                                                                                                                                                                                                  >  2:188195/1‑242 (MQ=255)         tctcATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGt                                                                                                                                                                                                                                                  <  1:188195/243‑1 (MQ=255)          ctcATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGGGAGTGGTTCGGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCCATGCAAGAGGCGGGGTGGTGAAGATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAg                                                                                                                                                                                                                                           <  1:383901/249‑1 (MQ=255)            cATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCCCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGGGAATTTCATCACGACGCGCGATGAACCCGGAACGGAAATTTCGGGGGGGGGGGGAAGCaa                                                                                                                                                                                                                                        >  1:171567/1‑250 (MQ=255)                 aTTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATa                                                                                                                                                                                                                                      <  1:13017/247‑1 (MQ=255)                           gcgGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                                                                                                                                                                                        >  1:199588/1‑251 (MQ=255)                                        cAGTCATGTGTTGGTGTTCATTGCTGGCGTCCGCCTGAATCTGGCGGGGGTGAGTGGTTATGCCGACCTTAGCCCGTTCCCTATTGCGCTTGTCAAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGTGAATATCATAACGACGCGCGATGAACCCGGAAAGGAAATTTAAGGAGGGGGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgc                                                                                                                                                                                                           >  2:258322/1‑251 (MQ=255)                                                       gtTAATTGATGGCTTTCGCCTGAAGCTTGCGGGGGTGAGTGGTTCTGCCGACCTTATCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTTAATAGAGCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATATGCGGGGTGGTGAATATCATCACGACGCGCTATGAACCCTGAACTGAAATTTCAGGAGGGTGTGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTggggg                                                                                                                                                                                            <  1:255910/251‑1 (MQ=255)                                                       gtTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgggg                                                                                                                                                                                             <  1:254878/250‑1 (MQ=255)                                                                    gTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGt                                                                                                                                                                               >  1:228362/1‑251 (MQ=255)                                                                     tACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGGGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCCGAACTATGATGTCTCTACGCAGCAACAAATGGGGGATAAGACACGGGt                                                                                                                                                                               >  1:6164/1‑250 (MQ=255)                                                                             aaTCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACTGGTAACGCTGtt                                                                                                                                                                      >  2:82739/1‑251 (MQ=255)                                                                                  ggCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc                                                                                                                                                                  <  2:151062/250‑1 (MQ=255)                                                                                     gggggTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt                                                                                                                                                              >  2:123265/1‑251 (MQ=255)                                                                                        ggTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATg                                                                                                                                                           <  1:228150/251‑1 (MQ=255)                                                                                                     gCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATg                                                                                                                                               <  1:279664/250‑1 (MQ=255)                                                                                                            ttAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTatgat                                                                                                                                       >  1:367228/1‑251 (MQ=255)                                                                                                               gCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGAtgtt                                                                                                                                    <  1:224903/251‑1 (MQ=255)                                                                                                                 cAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGAtgttgt                                                                                                                                  >  2:72019/1‑251 (MQ=255)                                                                                                                  aGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGAGCTACGCTGTTTATGGTTCCGATGCAATCGGCGGGGTGGCGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTAAGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTTATGGTTATGATgttgtt                                                                                                                                 >  1:231049/1‑251 (MQ=255)                                                                                                                       ccTATTGCGGTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTGCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGAGGTCGCTACGCAGCAACAACTGGGGGATAAGACACGGGGAACGCGGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTa                                                                                                                            <  1:381747/251‑1 (MQ=255)                                                                                                                              cgcTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAAt                                                                                                                     >  1:274372/1‑251 (MQ=255)                                                                                                                                           tgtTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCaa                                                                                                         <  2:40036/250‑1 (MQ=255)                                                                                                                                            gtTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAg                                                                                                        <  2:90299/250‑1 (MQ=255)                                                                                                                                                 atatatCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGTAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTTGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGTACGCAAgcgc                                                                                                     >  1:198656/1‑248 (MQ=255)                                                                                                                                                   atatccgagagtcgcgCTCCGCTGTTTATGGTTCCGATGAAATAGGCGGGGTGGAGAATATCATCACGACCCGCGATGAAACCGGACCGGAAATTTTAGGAGGGTGAGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGAAACAACTGGGGGAAAAGACACGGGTAACGCTGTTGGGCGGTTATGCCCCTACTCATGGTTATGATTTTGGTGGCTATGGTAATACCGGAACGCAAGCGcagac                                                                                                 >  2:328363/13‑250 (MQ=255)                                                                                                                                                           gggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGAGCAGACAGATAACGa                                                                                        <  1:119111/251‑1 (MQ=255)                                                                                                                                                               cgcgCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGt                                                                                    <  1:133778/251‑1 (MQ=255)                                                                                                                                                               cgcgCTCCGCTGTTTAAGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGGGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTGGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAAGACCGGAACGTATGCGCAGACAGATAACTATGGt                                                                                    <  2:269320/251‑1 (MQ=255)                                                                                                                                                                   cTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTCTGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGttttt                                                                                >  2:365121/1‑251 (MQ=255)                                                                                                                                                                          gTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTaa                                                                         <  1:183850/251‑1 (MQ=255)                                                                                                                                                                             tATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTTGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAAc                                                                      >  1:107947/1‑251 (MQ=255)                                                                                                                                                                             tATGGTTCCGATGCAATAGACGGGGAGGTAAATATGATCCCCACGCGCGATGAACCCGGAACGGAACTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTCTGATGTCTCTACGCCCCAACAACCGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGCTGTTGTTGCCTATGGTAATACCGGCACGCAAGCGCAGACAGATAACGATGGTTTTTTACGTAAAAc                                                                      >  2:175739/1‑251 (MQ=255)                                                                                                                                                                              aTGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACg                                                                     <  1:359989/251‑1 (MQ=255)                                                                                                                                                                                     cGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAGGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGGAAAACTCTTTATg                                                              <  2:65957/251‑1 (MQ=255)                                                                                                                                                                                     cGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACACGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATg                                                              <  1:11109/251‑1 (MQ=255)                                                                                                                                                                                      gATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATaa                                                                                           <  2:114514/221‑1 (MQ=255)                                                                                                                                                                                      gATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATaa                                                                                           >  1:114514/1‑221 (MQ=255)                                                                                                                                                                                       aTGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGgc                                                            <  2:171567/251‑1 (MQ=255)                                                                                                                                                                                              aGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTg                                                      <  1:104316/250‑1 (MQ=255)                                                                                                                                                                                                  ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc                                                                                                                                                                  >  2:49618/1‑138 (MQ=255)                                                                                                                                                                                                  ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGc                                                                                                                                                                  <  1:49618/138‑1 (MQ=255)                                                                                                                                                                                                  ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGCAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGc                                                  >  2:280446/1‑250 (MQ=255)                                                                                                                                                                                                                catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATg                                   <  1:72019/251‑1 (MQ=255)                                                                                                                                                                                                                                          cggaaATTTCAGGAGGGAGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCggctat         >  1:352302/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCggctatggc      <  1:290326/251‑1 (MQ=255)                                                                                                                                                                                                                                                  tCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTATAGCGGCTTTGTGCGCGgctatggctatg  <  2:28407/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AGGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTGGTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATG  >  NC_000913/4163878‑4164368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑