Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,277	G→A	R188H (CGT→CAT)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,277	0	G	A	98.2%	175.8 / NA	54	R188H (CGT→CAT)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (26/27);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCAT  >  NC_000913/277038‑277518                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   agagAACCCGCAGGCGGGATCGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGTATGCCGGGGCCGATCTGCTGGCCTGCTAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGt                                                                                                                                                                                                                                         <  2:413308/251‑1 (MQ=255)           cAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCACGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGGTCTCAtt                                                                                                                                                                                                                               >  2:356308/1‑251 (MQ=255)                 gcaaCGCTTCTGGTGGCGGGGTCCGGCGGGCCTTACGGCGCGGACCTGGCGGATGGCTCCGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGGTTCATCGCCCGCGCGTGGGAGCCTTGCTGTAAGCCGGGGGCGGTCTGCTGGCCTGCGAAACCCTGCCGCATTTTTCCGAGCTTTAGGCGTTTGCCCTGCTGTTGACCGCCTATCCACATGGGCGGGCGGTGTTCTCATTTAcccc                                                                                                                                                                                                                         >  2:221642/3‑250 (MQ=255)                   aaCGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTAccc                                                                                                                                                                                                                          >  1:383796/1‑248 (MQ=255)                   aaCGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCAGTTAccc                                                                                                                                                                                                                          <  2:383796/248‑1 (MQ=255)                               ggCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGaa                                                                                                                                                                                                            <  2:247799/250‑1 (MQ=255)                                    gATCCGTCGGGCCTTACGGCGCGTATCTGGCGGACGGCTCTGAATACCGTGGCGATTCTCATTGTAGCGTTGAGGCATTTCAGGAGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTTACCGAATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTg                                                                                                                                                                                                      >  2:284226/1‑251 (MQ=255)                                           cGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACg                                                                                                                                                                                               >  1:280284/1‑251 (MQ=255)                                           cGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACg                                                                                                                                                                                               <  2:34517/251‑1 (MQ=255)                                                     ggCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCt                                                                                                                                                                                     <  1:342841/251‑1 (MQ=255)                                                     ggCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCt                                                                                                                                                                                     <  1:342825/251‑1 (MQ=255)                                                          gTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTg                                                                                                                                                                                <  1:272476/251‑1 (MQ=255)                                                           tATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTg                                                                                                                                                                                >  2:405237/1‑250 (MQ=255)                                                                          tctGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCt                                                                                                                                                                >  1:241940/1‑251 (MQ=255)                                                                          tctGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATAGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGTCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCt                                                                                                                                                                >  1:269503/1‑251 (MQ=255)                                                                           ctGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGgttgcgttgc                                                                                                                                                                 <  1:150555/249‑1 (MQ=255)                                                                                 aCCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCggg                                                                                                                                                          <  2:386796/250‑1 (MQ=255)                                                                                      ggCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATcc                                                                                                                                                    >  2:352739/1‑251 (MQ=255)                                                                                            tATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGACCACCTGAGCGACGGTACGCCGCt                                                                                                                                                                                     >  2:358973/1‑212 (MQ=255)                                                                                            tATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCt                                                                                                                                                                                     <  1:358973/212‑1 (MQ=255)                                                                                            gaTCATGGTAGCGTGGAGTAATTTCAGGCGTTTCAGCGCCCGCGGGTGTAAGCCTTGCTGGATGCCGGGGCCGAGCTGCGTTCCTGCGAAACCCTGCCGAAGTTTTCCGAGATTGAGGCGGTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCGCATTTACCCTGCGCGGCAGCGAACGCCTGAGCGACGGTACGCCGGTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑ggt                                                                                                                                             <  2:195885/250‑1 (MQ=255)                                                                                               cATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑ggtggt                                                                                                                                          <  1:359020/251‑1 (MQ=255)                                                                                                   gTAGCGTTGAGGCATTTCAGGCGTGTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGgcg                                                                                                                                      <  2:369760/251‑1 (MQ=255)                                                                                                     ggcggtGAGGCATTTCAGGCGTTTCAGCGCCCGCGCGTGGAAGCCGTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCt                                                                                                                                    <  2:148025/246‑1 (MQ=255)                                                                                                          tGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGGGGCGCTAGGc                                                                                                                                >  1:96388/1‑250 (MQ=255)                                                                                                                aTTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGCGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGGTATTCGCA‑GGGGGGGGCGCTCGGCATTTACt                                                                                                                         >  2:139952/1‑251 (MQ=255)                                                                                                                   tCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGt                                                                                                                       <  2:106965/250‑1 (MQ=255)                                                                                                                      ggCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGCAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGGGGGGGCGCTAGGCATTAAATGTAttt                                                                                                                   >  2:384542/1‑250 (MQ=255)                                                                                                                             cATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGGGGCGCCACGCATTAACTGTATTGCGCTggg                                                                                                            >  2:138128/1‑250 (MQ=255)                                                                                                                              aTCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTCGCTGGCGGGGTATCCGCC‑GGTGGTGGGGTCAGGCATTAACTGTATTGCGGTggg                                                                                                            >  2:326940/1‑249 (MQ=255)                                                                                                                                 gCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAac                                                                                                        <  1:82491/251‑1 (MQ=255)                                                                                                                                  cccGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGGGGGGGCGCTAGGCATTAACTGTATTGCGCTGGAAAac                                                                                                        >  1:247270/1‑250 (MQ=255)                                                                                                                                     gcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGtggctggc                                                                                                                                                             <  1:318294/195‑1 (MQ=255)                                                                                                                                     gcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGc                                                                                                                                                             >  2:318294/1‑195 (MQ=255)                                                                                                                                     gcgcgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAAcacc                                                                                                     >  1:376419/1‑250 (MQ=255)                                                                                                                                        cgTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACaccaccc                                                                                                 >  1:134847/1‑250 (MQ=255)                                                                                                                                         gTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGGTATCCGCC‑GGGGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACAACACCGc                                                                                                >  2:78498/1‑251 (MQ=255)                                                                                                                                            gAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGc                                                                                             <  2:408840/251‑1 (MQ=255)                                                                                                                                            gAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGc                                                                                             <  1:78498/251‑1 (MQ=255)                                                                                                                                               gCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGtt                                                                                          <  1:385340/251‑1 (MQ=255)                                                                                                                                                              ggggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTgcgc                                                                                                                                                                                                                     <  2:82826/114‑1 (MQ=255)                                                                                                                                                              ggggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTgcgc                                                                                                                                                                                                                     >  1:82826/1‑114 (MQ=255)                                                                                                                                                                ggCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCGGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGtt                                                                         <  1:284226/251‑1 (MQ=255)                                                                                                                                                                            ggCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGGGGTGGCGCTAGGCATTCACTGTATTGCGCCGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGtgctgc                                                             >  2:67649/1‑251 (MQ=255)                                                                                                                                                                              ccTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCg                                                           <  2:96388/251‑1 (MQ=255)                                                                                                                                                                                     aaCCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTGGACCGCATATCCGCATGCGCGGGCGTGGTGCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTTCGTGACGTGGTTGCTTTGCTGGCGGGGTATCCGCAAGGTGG‑GGCGCTAGGCATGAACTGGATTGTGCTGGAAAACACCCCCGCTGGGGTGCAGGATTTACACGGTTTAACGGTGCGGcccgtggggg                                                    >  1:276/1‑243 (MQ=255)                                                                                                                                                                                      aCCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGGGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCCGCATTTTCACGGTTTAACGGTGCTGCCGCtgggggg                                                   >  2:356621/1‑247 (MQ=255)                                                                                                                                                                                            ccGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATcc                                             <  1:106016/251‑1 (MQ=255)                                                                                                                                                                                                     tCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTACTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCgg                                     <  2:287391/250‑1 (MQ=255)                                                                                                                                                                                                          gaTTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGc                               >  1:88175/1‑251 (MQ=255)                                                                                                                                                                                                                               gTTGACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATTCCGTGAGCaaa           >  1:349231/1‑250 (MQ=255)                                                                                                                                                                                                                                  gACCGCATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGGTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAAcc        <  2:398106/250‑1 (MQ=255)                                                                                                                                                                                                                                     cGCATAGCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGc    <  2:241940/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATATCCGCATGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACAGCTGGGCGACGGTACGCCGCTGCGTGGCGAGGTGGCGTGGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGcat  <  2:318287/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   AGAGAACCCGCAGGCGGGAACGCTTCTGGTGGCGGGATCCGTCGGGCCTTACGGCGCGTATCTGGCGGATGGCTCTGAATACCGTGGCGATTATCATTGTAGCGTTGAGGCATTTCAGGCGTTTCATCGCCCGCGCGTGGAAGCCTTGCTGGATGCCGGGGCCGATCTGCTGGCCTGCGAAACCCTGCCGAATTTTTCCGAGATTGAGGCGTTGGCCGAGCTGTTGACCGCATATCCGCGTGCGCGGGCGTGGTTCTCATTTACCCTGCGCGACAGCGAACACCTGAGCGACGGTACGCCGCTGCGTGACGTGGTTGCGTTGCTGGCGGGTTATCCGCA‑GGTGGTGGCGCTAGGCATTAACTGTATTGCGCTGGAAAACACCACCGCTGCGTTGCAGCATTTACACGGTTTAACGGTGCTGCCGCTGGTGGTGTATCCGAACTCGGGCGAGCATTACGATGCCGTGAGCAAAACCTGGCAT  >  NC_000913/277038‑277518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑